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Lactobacillus equi sp. nov., a predominant intestinal Lactobacillus species of the horse isolated from faeces of healthy horses.
Abstract: Lactobacillus equi sp. nov. is described on the basis of 18 strains isolated as one of the predominant intestinal lactobacilli from horse faecal specimens. These 18 strains were isolated from 10 horses of 6 different farms out of 20 horses of 10 farms examined. They were gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming, non-motile, lactic-acid-homofermentative rods. The DNA G+C content was 38.9+/-0.8 mol %. DNA-DNA hybridization failed to associate these strains closely with any of the validly described type strains used. Analysis of the 16S rRNA gene sequence of representative strain YIT 0455T revealed that the new isolates represent a new Lactobacillus species, for which the name Lactobacillus equi is proposed. The type strain is YIT 0455T (= ATCC BAA-261T = JCM 10991T).
Publication Date: 2002-02-12 PubMed ID: 11837305DOI: 10.1099/00207713-52-1-211Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The researchers discovered and described a new species of Lactobacillus, a dominant intestinal bacteria found in horses, named Lactobacillus equi sp. nov., isolated from the feces of healthy horses.
Research Method and Findings
- The research revolved around the identification and classification of a previously unknown Lactobacillus species. The Lactobacillus bacteria help in maintaining a healthy gut microbiota.
- A total of 18 strains of this new species were isolated from the fecal samples of healthy horses. The samples were collected from 10 horses picked from six different farms, which were part of a larger group of 20 horses from 10 different farms.
- The strains were found to be gram-positive, meaning they can be stained violet under a Gram stain test. They lack spores and motion, and also found to be facultatively anaerobic, implying they can survive with or without oxygen. Additionally, they were catalase-negative, indicating they don’t produce the enzyme catalase.
- The new strains were identified as a homofermentative rod, suggesting they ferment sugars into lactic acid as the primary product and take the form of non-tapered, small rod-like structures.
- The DNA G+C content (a measure of the guanine-cytosine composition in DNA) was approximately 38.9%.
Naming the New Species
- These new strains did not match closely with any previously-described type strains in DNA-DNA hybridization, which is a method to check genetic similarity between two microbial species.
- They analyzed the 16S rRNA gene sequence (a method for identifying and classifying bacteria) of a representative strain YIT 0455T and confirmed that the strain represented a new species under Lactobacillus.
- They named this new species as Lactobacillus equi based on its horse origin. YIT 0455T was designated as the “type strain” for this species so that it will be used as a reference for identifying Lactobacillus equi in the future.
Cite This Article
APA
Morotomi M, Yuki N, Kado Y, Kushiro A, Shimazaki T, Watanabe K, Yuyama T.
(2002).
Lactobacillus equi sp. nov., a predominant intestinal Lactobacillus species of the horse isolated from faeces of healthy horses.
Int J Syst Evol Microbiol, 52(Pt 1), 211-214.
https://doi.org/10.1099/00207713-52-1-211 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Bacterial Typing Techniques
- DNA, Ribosomal / analysis
- Feces / microbiology
- Horses / microbiology
- Lactobacillus / classification
- Lactobacillus / genetics
- Lactobacillus / isolation & purification
- Lactobacillus / metabolism
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Phylogeny
- RNA, Ribosomal, 16S / genetics
- Sequence Analysis, DNA
Citations
This article has been cited 11 times.- Li XB, Huang XX, Li Q, Li XY, Li JH, Li C, He LJ, Jing HX, Yang KL. Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach. BMC Vet Res 2022 Nov 17;18(1):407.
- Li Y, Liu C, Liu Q, Liu W. Comparative Genomic Analysis Reveals Intestinal Habitat Adaptation of Ligilactobacillus equi Rich in Prophage and Degrading Cellulase. Molecules 2022 Mar 14;27(6).
- Kauter A, Epping L, Semmler T, Antao EM, Kannapin D, Stoeckle SD, Gehlen H, Lübke-Becker A, Günther S, Wieler LH, Walther B. The gut microbiome of horses: current research on equine enteral microbiota and future perspectives. Anim Microbiome 2019 Nov 13;1(1):14.
- Silva BC, Sandes SH, Alvim LB, Bomfim MR, Nicoli JR, Neumann E, Nunes AC. Selection of a candidate probiotic strain of Pediococcus pentosaceus from the faecal microbiota of horses by in vitro testing and health claims in a mouse model of Salmonella infection. J Appl Microbiol 2017 Jan;122(1):225-238.
- Sickel W, Grafe TU, Meuche I, Steffan-Dewenter I, Keller A. Bacterial Diversity and Community Structure in Two Bornean Nepenthes Species with Differences in Nitrogen Acquisition Strategies. Microb Ecol 2016 May;71(4):938-53.
- Tanabe S, Suzuki T, Wasano Y, Nakajima F, Kawasaki H, Tsuda T, Nagamine N, Tsurumachi T, Sugaya K, Akita H, Takagi M, Takagi K, Inoue Y, Asai Y, Morita H. Anti-inflammatory and Intestinal Barrier-protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds. J Equine Sci 2014;25(2):37-43.
- O'Donnell MM, Harris HM, O'Toole PW, Ross RP. The Genome of the Predominant Equine Lactobacillus Species, Lactobacillus equi, Is Reflective of Its Lifestyle Adaptations to an Herbivorous Host. Genome Announc 2014 Jan 16;2(1).
- St-Pierre B, de la Fuente G, O'Neill S, Wright AD, Al Jassim R. Analysis of stomach bacterial communities in Australian feral horses. Mol Biol Rep 2013 Jan;40(1):369-76.
- Morita H, Shiratori C, Murakami M, Takami H, Kato Y, Endo A, Nakajima F, Takagi M, Akita H, Okada S, Masaoka T. Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds. Int J Syst Evol Microbiol 2007 Dec;57(Pt 12):2836-2839.
- Li H, Bao L, Wang T, Guan Y. Dietary change influences the composition of the fecal microbiota in two rescued wild raccoon dogs (Nyctereutes procyonoides). Front Microbiol 2024;15:1335017.
- Cain JL, Norris JK, Swan MP, Nielsen MK. A diverse microbial community and common core microbiota associated with the gonad of female Parascaris spp. Parasitol Res 2023 Dec 18;123(1):56.
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