Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse.
Abstract: We previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). Of the five strains, four were identified as members of existing Lactobacillus species; however, sequence analysis of the 16S rRNA gene revealed that the fifth isolate, DI70(T), showed approximately 97 % identity (1325/1366 bp) with the type strain of Lactobacillus delbrueckii. Therefore, we considered the possibility that DI70(T) represents a novel species of the genus Lactobacillus. Cells of strain DI70(T) were Gram-stain-positive, catalase-negative, non-spore-forming, non-motile rods. In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70(T) formed a subcluster in the L. delbrueckii phylogenetic group and was closely related to L. delbrueckii, Lactobacillus crispatus and Lactobacillus jensenii. However, analysis of DNA-DNA relatedness showed that DI70(T) was genetically distinct from its phylogenetic relatives. The isolate also exhibited distinct biochemical and physiological characteristics when compared with its phylogenetic relatives. It required anaerobic conditions for growth on agar medium. The results indicate that isolate DI70(T) indeed represents a novel species of the genus Lactobacillus, for which we propose the name Lactobacillus equicursoris sp. nov. The type strain is DI70(T) (=JCM 14600(T) =DSM 19284(T)).
Publication Date: 2009-07-31 PubMed ID: 19648335DOI: 10.1099/ijs.0.009290-0Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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In this study, researchers identified a new species of Lactobacillus, named Lactobacillus equicursoris, isolated from the feces of a thoroughbred racehorse.
Background
- The researchers had initially isolated five strains of putative lactobacilli from the feces of a 4-year-old male thoroughbred racehorse.
- Out of these five strains, four were identified as members of existing Lactobacillus species. However, the fifth strain dubbed DI70(T) showed roughly 97% identity with the strain of Lactobacillus delbrueckii as revealed by the 16S rRNA gene sequence analysis.
Isolate DI70(T) Characteristics
- The cells of strain DI70(T) were found to be Gram-stain-positive, catalase-negative, non-spore-forming, and non-motile rods.
- In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70(T) formed a subcluster in the L. delbrueckii phylogenetic group.
- It showed a close relation to L. delbrueckii, Lactobacillus crispatus and Lactobacillus jensenii.
Distinctiveness of Isolate DI70(T)
- Despite its close relation to other species, analysis of DNA-DNA relatedness showed that DI70(T) was genetically distinct from its phylogenetic relatives.
- The isolate also showed distinct biochemical and physiological characteristics when compared with its phylogenetic relatives.
- One important distinction was that it required anaerobic conditions for growth on agar medium.
- The results led the researchers to the conclusion that isolate DI70(T) represented a novel species of the genus Lactobacillus.
- They proposed to name this new species Lactobacillus equicursoris sp. nov. The type strain is DI70(T) (=JCM 14600(T) =DSM 19284(T)).
Cite This Article
APA
Morita H, Shimazu M, Shiono H, Toh H, Nakajima F, Akita H, Takagi M, Takami H, Murakami M, Masaoka T, Tanabe S, Hattori M.
(2009).
Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse.
Int J Syst Evol Microbiol, 60(Pt 1), 109-112.
https://doi.org/10.1099/ijs.0.009290-0 Publication
Researcher Affiliations
- School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
- School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
- School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
- RIKEN Advanced Science Institute, 1-7-22 Suehiro, Tsurumi, Yokohama, Kanagawa 230-0045, Japan.
- Northern Farm, 275 Hayakita-genbu, Abira-cho, Yufutsu-gun, Hokkaido 059-1432, Japan.
- Northern Farm, 275 Hayakita-genbu, Abira-cho, Yufutsu-gun, Hokkaido 059-1432, Japan.
- Crossfield-Bio Inc., 1-1-20 Higashi-nihonbashi, Chuo, Tokyo 103-0004, Japan.
- Microbial Genome Research Group, Japan Agency of Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan.
- School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
- School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
- Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan.
- Graduate School of Frontier Sciences, University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.
MeSH Terms
- Animals
- DNA, Bacterial / genetics
- DNA, Ribosomal / genetics
- Feces / microbiology
- Horses
- Lactobacillus / classification
- Lactobacillus / genetics
- Lactobacillus / isolation & purification
- Lactobacillus / metabolism
- Male
- Molecular Sequence Data
- Phylogeny
- RNA, Ribosomal, 16S / genetics
Citations
This article has been cited 6 times.- Li XB, Huang XX, Li Q, Li XY, Li JH, Li C, He LJ, Jing HX, Yang KL. Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach. BMC Vet Res 2022 Nov 17;18(1):407.
- Barco RA, Garrity GM, Scott JJ, Amend JP, Nealson KH, Emerson D. A Genus Definition for Bacteria and Archaea Based on a Standard Genome Relatedness Index. mBio 2020 Jan 14;11(1).
- Ueda F, Iida A, Saito H, Seki S, Amao A, Yamate H. Assessment of the effect and safety of salacinol in horses. J Equine Sci 2019;30(4):105-111.
- Sun Y, Tamarit D, Andersson SGE. Switches in Genomic GC Content Drive Shifts of Optimal Codons under Sustained Selection on Synonymous Sites. Genome Biol Evol 2017 Oct 1;9(10):2560-2579.
- Tanabe S, Suzuki T, Wasano Y, Nakajima F, Kawasaki H, Tsuda T, Nagamine N, Tsurumachi T, Sugaya K, Akita H, Takagi M, Takagi K, Inoue Y, Asai Y, Morita H. Anti-inflammatory and Intestinal Barrier-protective Activities of Commensal Lactobacilli and Bifidobacteria in Thoroughbreds: Role of Probiotics in Diarrhea Prevention in Neonatal Thoroughbreds. J Equine Sci 2014;25(2):37-43.
- Cousin S, Loux V, Ma L, Creno S, Clermont D, Bizet C, Bouchier C. Draft Genome Sequences of Lactobacillus equicursoris CIP 110162T and Lactobacillus sp. Strain CRBIP 24.137, Isolated from Thoroughbred Racehorse Feces and Human Urine, Respectively. Genome Announc 2013 Aug 22;1(4).
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