Lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses.
Abstract: Two strains of lactic acid bacteria were isolated from faeces of two actively racing thoroughbred horses. The isolates formed a subcluster in the Lactobacillus reuteri phylogenetic group, closely related to Lactobacillus fermentum, L. gastricus, L. ingluviei and L. mucosae, by phylogenetic analysis based on 16S rRNA gene sequences. Levels of DNA-DNA relatedness revealed that the isolates belonged to the same taxon and were genetically separated from their phylogenetic relatives. Biochemical and physiological characteristics also distinguished the isolates from their phylogenetic relatives. The isolates produced spherical or oval cells, and tetrad-like cells were rarely seen. To the best of our knowledge, this is the first report of this morphological characteristic within the genus Lactobacillus. Thus, the isolates represent an atypical novel species of the genus Lactobacillus, for which the name Lactobacillus equigenerosi sp. nov. is proposed. The type strain is NRIC 0697T (=JCM 14505T =DSM 18793T).
Publication Date: 2008-04-10 PubMed ID: 18398194DOI: 10.1099/ijs.0.65250-0Google Scholar: Lookup
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- Journal Article
Summary
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This study discovered and classified a new bacterium species, Lactobacillus equigenerosi sp. nov., found in the faeces of racing thoroughbred horses. The new species has unique characteristics, including its genetic separation from other closely related bacteria and atypical physical characteristics.
Isolation and Identification
- Researchers isolated two strains of lactic acid bacteria from the faeces of two actively racing thoroughbred horses.
- The bacterium was identified using phylogenetic analysis based on 16S rRNA gene sequences. The analyis revealed it was closely related to Lactobacillus fermentum, L. gastricus, L. ingluviei and L. mucosae, but formed its own subcluster in the Lactobacillus reuteri phylogenetic group.
DNA-DNA Relatedness
- The level of DNA-DNA relatedness among the two isolates showed that they belonged to the same taxon, indicating that they were of the same species.
- The DNA-DNA relatedness data also demonstrated these isolates were genetically separated from their phylogenetic relatives, meaning they form a new and distinct Lactobacillus species.
Biochemical and Physiological Characteristics
- The isolates’ biochemical and physiological characteristics also set them apart from their closest phylogenetic relatives.
- The physical appearance of the bacteria cells was different – producing spherical or oval cells, with tetrad-like cells being rarely seen. The researchers highlight this morphological feature as being unique within the Lactobacillus genus.
Classification and Naming
- Based on the genetic, biochemical and physiological differences, the researchers classified the bacteria as a new species – the first of its kind in the Lactobacillus genus – naming it Lactobacillus equigenerosi sp. nov.
- The study defines the type strain for this new species as NRIC 0697T (=JCM 14505T =DSM 18793T).
Cite This Article
APA
Endo A, Roos S, Satoh E, Morita H, Okada S.
(2008).
Lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses.
Int J Syst Evol Microbiol, 58(Pt 4), 914-918.
https://doi.org/10.1099/ijs.0.65250-0 Publication
Researcher Affiliations
- NODAI Culture Collection Center, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan. pegaman@hotmail.co.jp
MeSH Terms
- Animals
- Feces / microbiology
- Genes, Bacterial
- Horses / microbiology
- Lactobacillus / classification
- Lactobacillus / genetics
- Lactobacillus / isolation & purification
- Lactobacillus / metabolism
- Microscopy, Electron, Scanning
- Molecular Sequence Data
- Phenotype
- Phylogeny
- RNA, Bacterial / genetics
- RNA, Ribosomal, 16S / genetics
Citations
This article has been cited 12 times.- Li XB, Huang XX, Li Q, Li XY, Li JH, Li C, He LJ, Jing HX, Yang KL. Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach. BMC Vet Res 2022 Nov 17;18(1):407.
- Gilroy R, Leng J, Ravi A, Adriaenssens EM, Oren A, Baker D, La Ragione RM, Proudman C, Pallen MJ. Metagenomic investigation of the equine faecal microbiome reveals extensive taxonomic diversity. PeerJ 2022;10:e13084.
- Suzuki S, Fujita K, Maeno S, Shiwa Y, Endo A, Yokota K, Igimi S, Kajikawa A. PCR-based screening, isolation, and partial characterization of motile lactobacilli from various animal feces. BMC Microbiol 2020 Jun 3;20(1):142.
- Toh H, Nakano A, Nguyen CT, Mimura I, Arakawa K, Tashiro K, Kikusui T, Morita H. Draft Genome Sequence of Coccoid Lactobacillus equigenerosi NRIC 0697T Isolated from the Gastrointestinal Tracts of Healthy Thoroughbreds. Genome Announc 2016 Feb 4;4(1).
- Martín V, Cárdenas N, Jiménez E, Maldonado A, Rodríguez JM, Fernández L. Genome Sequence of Lactobacillus gastricus PS3, a Strain Isolated from Human Milk. Genome Announc 2013 Jul 11;1(4).
- St-Pierre B, de la Fuente G, O'Neill S, Wright AD, Al Jassim R. Analysis of stomach bacterial communities in Australian feral horses. Mol Biol Rep 2013 Jan;40(1):369-76.
- Botha M, Botes M, Loos B, Smith C, Dicks LM. Lactobacillus equigenerosi strain Le1 invades equine epithelial cells. Appl Environ Microbiol 2012 Jun;78(12):4248-55.
- Perkins GA, den Bakker HC, Burton AJ, Erb HN, McDonough SP, McDonough PL, Parker J, Rosenthal RL, Wiedmann M, Dowd SE, Simpson KW. Equine stomachs harbor an abundant and diverse mucosal microbiota. Appl Environ Microbiol 2012 Apr;78(8):2522-32.
- Endo A, Futagawa-Endo Y, Dicks LM. Lactobacillus and Bifidobacterium diversity in horse feces, revealed by PCR-DGGE. Curr Microbiol 2009 Dec;59(6):651-5.
- Ang Li Xuan A, Robert V, Hisatomi A, Hirasaki M, Maeno S, Ohkuma M, Sakamoto M, Chatel JM, Endo A. Faecalibacterium langellae sp. nov. isolated from human faeces. Int J Syst Evol Microbiol 2025 Jun;75(6).
- Cain JL, Norris JK, Swan MP, Nielsen MK. A diverse microbial community and common core microbiota associated with the gonad of female Parascaris spp. Parasitol Res 2023 Dec 18;123(1):56.
- Paul LJ, Ericsson AC, Andrews FM, McAdams Z, Keowen ML, St Blanc MP, Banse HE. Field study examining the mucosal microbiome in equine glandular gastric disease. PLoS One 2023;18(12):e0295697.
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