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Home / Equine Research Bank / Appl Environ Microbiol / Lactobacillus equigenerosi strain Le1 invades equine epithel...
Applied and environmental microbiology2012; 78(12); 4248-4255; doi: 10.1128/AEM.00552-12

Lactobacillus equigenerosi strain Le1 invades equine epithelial cells.

Abstract: Lactobacillus equigenerosi strain Le1, a natural inhabitant of the equine gastrointestinal tract, survived pH 3.0 and incubation in the presence of 1.5% (wt/vol) bile salts for at least 2 h. Strain Le1 showed 8% cell surface hydrophobicity, 60% auto-aggregation, and 47% coaggregation with Clostridium difficile C6. Only 1% of the cells adhered to viable buccal epithelial cells and invaded the cells within 20 min after contact. Preincubation of strain Le1 in a buffer containing pronase prevented adhesion to viable epithelial cells. Preincubation in a pepsin buffer delayed invasion from 20 min to 1 h. Strain Le1 did not adhere to nonviable epithelial cells. Administration of L. equigenerosi Le1 (1 × 10(9) CFU per 50 kg body weight) to healthy horses did not increase white blood cell numbers. Differential white blood cell counts and aspartate aminotransferase levels remained constant. Glucose, lactate, cholesterol, and urea levels remained constant during administration with L. equigenerosi Le1 but decreased during the week after administration.
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Publication Date: 2012-04-13 PubMed ID: 22504808PubMed Central: PMC3370564DOI: 10.1128/AEM.00552-12Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores how Lactobacillus equigenerosi strain Le1, a bacteria found in horse intestines, can survive harsh conditions, stick to cells, and then invade them. The study also suggests administering this bacteria to healthy horses doesn’t alter many of their common health indicators.

Methodology and Findings

  • The researchers first studied the survival capabilities of L. equigenerosi strain Le1 by subjecting it to difficult conditions for the survival of bacteria, such as a pH of 3.0 and in the presence of 1.5% bile salts. They found that this bacteria could survive these harsh conditions for at least 2 hours.
  • The researchers next looked at the ability of strain Le1 to interact with other cells. They found that this bacteria had about 8% cell surface hydrophobicity, which indicates its capacity to attach to surfaces of cells or other particles, 60% auto-aggregation, which is the ability to cluster or aggregate with its own kind, and 47% coaggregation with another bacteria, Clostridium difficile C6, indicating its ability to attach to different bacterial species.
  • The study showed that only 1% of the bacterial cells could bind to healthy epithelial cells in the mouth of horses and could invade these cells within 20 minutes after contact. This invasion was prevented if the bacteria were preincubated in a buffer solution containing an enzyme known as pronase, and was delayed to 1 hour if the bacteria were incubated in a buffer containing another enzyme known as pepsin.
  • Importantly, the strain did not attach to nonviable epithelial cells, emphasizing its selectivity in adhering to healthy cells.

Effects on Horses

  • The researchers then examined the physiological response of horses to administering a high dose of L. equigenerosi Le1. They found that this did not increase the number of white blood cells (immunity cells) in healthy horses.
  • The levels of differential white blood cells, i.e., the percentage of each type of white blood cell in the blood, remained constant. Aspartate aminotransferase, an enzyme that can indicate liver damage when present at high levels, also did not change.
  • The levels of glucose, lactate, cholesterol, and urea, which are involved in energy metabolism, also remained constant during administration of L. equigenerosi Le1 but decreased during the week after administration.
  • This suggests that the presence of this bacteria in high quantities did not cause major alterations in the physiological parameters studied.

Cite This Article

APA
Botha M, Botes M, Loos B, Smith C, Dicks LM. (2012). Lactobacillus equigenerosi strain Le1 invades equine epithelial cells. Appl Environ Microbiol, 78(12), 4248-4255. https://doi.org/10.1128/AEM.00552-12

Publication

Applied and environmental microbiology
ISSN: 1098-5336
NlmUniqueID: 7605801
Country: United States
Language: English
Volume: 78
Issue: 12
Pages: 4248-4255

Researcher Affiliations

Botha, Marlie
  • Department of Microbiology, University of Stellenbosch, Stellenbosch, South Africa.
Botes, Marelize
    Loos, Ben
      Smith, Carine
        Dicks, Leon M T

          MeSH Terms

          • Animals
          • Antibiosis
          • Bacterial Adhesion
          • Clostridioides difficile / growth & development
          • Endocytosis
          • Epithelial Cells / microbiology
          • Horses
          • Lactobacillus / growth & development
          • Lactobacillus / physiology
          • Probiotics / administration & dosage

          References

          This article includes 37 references
          1. Al Jassim RA, Scott PT, Trebbin AL, Trott D, Pollitt CC. The genetic diversity of lactic acid producing bacteria in the equine gastrointestinal tract.. FEMS Microbiol Lett 2005 Jul 1;248(1):75-81.
            pubmed: 15953698doi: 10.1016/j.femsle.2005.05.023google scholar: lookup
          2. Arroyo LG, Staempfli H, Weese JS. Molecular analysis of Clostridium difficile isolates recovered from horses with diarrhea.. Vet Microbiol 2007 Feb 25;120(1-2):179-83.
            pubmed: 17112686doi: 10.1016/j.vetmic.2006.10.013google scholar: lookup
          3. Båverud V, Gustafsson A, Franklin A, Aspán A, Gunnarsson A. Clostridium difficile: prevalence in horses and environment, and antimicrobial susceptibility.. Equine Vet J 2003 Jul;35(5):465-71.
            pubmed: 12875324doi: 10.2746/042516403775600505google scholar: lookup
          4. Bonhomme-Florentin A. Degradation of hemicellulose and pectin by horse caecum contents.. Br J Nutr 1988 Jul;60(1):185-92.
            pubmed: 3408701doi: 10.1079/bjn19880087google scholar: lookup
          5. Botes M, Loos B, van Reenen CA, Dicks LM. Adhesion of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments.. Arch Microbiol 2008 Nov;190(5):573-84.
            pubmed: 18641972doi: 10.1007/s00203-008-0408-0google scholar: lookup
          6. Collado MC, Meriluoto J, Salminen S. Interactions between pathogens and lactic acid bacteria: aggregation and coaggregation abilities. Eur. J. Food Res. Technol. 226:1065–1073.
          7. de la Rebière de Pouyade G, Serteyn D. The role of activated neutrophils in the early stage of equine laminitis.. Vet J 2011 Jul;189(1):27-33.
            pubmed: 20655252doi: 10.1016/j.tvjl.2010.06.008google scholar: lookup
          8. Dicks LM, Botes M. Probiotic lactic acid bacteria in the gastro-intestinal tract: health benefits, safety and mode of action.. Benef Microbes 2010 Mar;1(1):11-29.
            pubmed: 21831747doi: 10.3920/bm2009.0012google scholar: lookup
          9. Doyle RJ, Rosenberg M. Measurement of microbial adhesion to hydrophobic substrata.. Methods Enzymol 1995;253:542-50.
            pubmed: 7476418doi: 10.1016/s0076-6879(95)53046-0google scholar: lookup
          10. Endo A, Okada S. Monitoring the lactic acid bacterial diversity during shochu fermentation by PCR-denaturing gradient gel electrophoresis.. J Biosci Bioeng 2005 Mar;99(3):216-21.
            pubmed: 16233780doi: 10.1263/jbb.99.216google scholar: lookup
          11. Endo A, Roos S, Satoh E, Morita H, Okada S. Lactobacillus equigenerosi sp. nov., a coccoid species isolated from faeces of thoroughbred racehorses.. Int J Syst Evol Microbiol 2008 Apr;58(Pt 4):914-8.
            pubmed: 18398194doi: 10.1099/ijs.0.65250-0google scholar: lookup
          12. Felske A, Rheims H, Wolterink A, Stackebrandt E, Akkermans ADL. Ribosome analysis reveals prominent activity of an uncultured member of the class Actinobacteria in grassland soils.. Microbiology (Reading) 1997 Sep;143 ( Pt 9):2983-2989.
            pubmed: 9308181doi: 10.1099/00221287-143-9-2983google scholar: lookup
          13. Frape D. 2010. Equine nutrition and feeding, 4th ed Wiley-Blackwell, Chichester, United Kingdom
          14. Gaggìa F, Mattarelli P, Biavati B. Probiotics and prebiotics in animal feeding for safe food production.. Int J Food Microbiol 2010 Jul 31;141 Suppl 1:S15-28.
            pubmed: 20382438doi: 10.1016/j.ijfoodmicro.2010.02.031google scholar: lookup
          15. Gopal PK, Prasad J, Smart J, Gill HS. In vitro adherence properties of Lactobacillus rhamnosus DR20 and Bifidobacterium lactis DR10 strains and their antagonistic activity against an enterotoxigenic Escherichia coli.. Int J Food Microbiol 2001 Aug 5;67(3):207-16.
            pubmed: 11518430doi: 10.1016/s0168-1605(01)00440-8google scholar: lookup
          16. Jones RL, Adney WS, Shideler RK. Isolation of Clostridium difficile and detection of cytotoxin in the feces of diarrheic foals in the absence of antimicrobial treatment.. J Clin Microbiol 1987 Jul;25(7):1225-7.
            pmc: PMC269181pubmed: 3112178doi: 10.1128/jcm.25.7.1225-1227.1987google scholar: lookup
          17. Kern DL, Slyter LL, Leffel EC, Weaver JM, Oltjen RR. Ponies vs. steers: microbial and chemical characteristics of intestinal ingesta.. J Anim Sci 1974 Mar;38(3):559-64.
            pubmed: 4856481doi: 10.2527/jas1974.383559xgoogle scholar: lookup
          18. Kirjavainen PV, Ouwehand AC, Isolauri E, Salminen SJ. The ability of probiotic bacteria to bind to human intestinal mucus.. FEMS Microbiol Lett 1998 Oct 15;167(2):185-9.
            pubmed: 9809419doi: 10.1111/j.1574-6968.1998.tb13226.xgoogle scholar: lookup
          19. Loguercio C, Del Vecchio Blanco C, Coltorti M. Enterococcus lactic acid bacteria strain SF68 and lactulose in hepatic encephalopathy: a controlled study.. J Int Med Res 1987 Nov-Dec;15(6):335-43.
            pubmed: 3125077doi: 10.1177/030006058701500602google scholar: lookup
          20. Madewell BR, Tang YJ, Jang S, Madigan JE, Hirsh DC, Gumerlock PH, Silva J Jr. Apparent outbreaks of Clostridium difficile-associated diarrhea in horses in a veterinary medical teaching hospital.. J Vet Diagn Invest 1995 Jul;7(3):343-6.
            pubmed: 7578449doi: 10.1177/104063879500700308google scholar: lookup
          21. Magdesian KG, Hirsh DC, Jang SS, Hansen LM, Madigan JE. Characterization of Clostridium difficile isolates from foals with diarrhea: 28 cases (1993-1997).. J Am Vet Med Assoc 2002 Jan 1;220(1):67-73.
            pubmed: 12680451doi: 10.2460/javma.2002.220.67google scholar: lookup
          22. Malik A, Sakamoto M, Hanazaki S, Osawa M, Suzuki T, Tochigi M, Kakii K. Coaggregation among nonflocculating bacteria isolated from activated sludge.. Appl Environ Microbiol 2003 Oct;69(10):6056-63.
            pmc: PMC201223pubmed: 14532062doi: 10.1128/aem.69.10.6056-6063.2003google scholar: lookup
          23. Morita H, Nakano A, Shimazu M, Toh H, Nakajima F, Nagayama M, Hisamatsu S, Kato Y, Takagi M, Takami H, Akita H, Matsumoto M, Masaoka T, Murakami M. Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds.. Anim Sci J 2009 Jun;80(3):339-46.
            pubmed: 20163646doi: 10.1111/j.1740-0929.2009.00633.xgoogle scholar: lookup
          24. Ouwehand AC, Kirjavainen PV, Grönland Isolauri M-ME, Salminen SJ. Adhesion of probiotic micro-organisms to intestinal mucus. Int. Dairy J. 9:623–630.
          25. Pagan JD. 1998. Advances in equine nutrition, 1st ed Nottingham University Press, Thrumpton, Nottingham, United Kingdom
          26. Patton K, Wright A, Kuroki K, Beard L. Hemorrhagic gastritis associated with renal failure, hemoglobinuria, and isolation of Clostridium perfringens in a horse. J. Equine Vet. Sci. 29:633–638.
          27. Ramiah K, van Reenen CA, Dicks LM. Surface-bound proteins of Lactobacillus plantarum 423 that contribute to adhesion of Caco-2 cells and their role in competitive exclusion and displacement of Clostridium sporogenes and Enterococcus faecalis.. Res Microbiol 2008 Jul-Aug;159(6):470-5.
            pubmed: 18619532doi: 10.1016/j.resmic.2008.06.002google scholar: lookup
          28. Reid G, McGroarty JA, Angotti R, Cook RL. Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens.. Can J Microbiol 1988 Mar;34(3):344-51.
            pubmed: 3138017doi: 10.1139/m88-063google scholar: lookup
          29. Rubino G. Hematology and some blood chemical parameters as a function of tick-borne disease (TBD) signs in horses. J. Equine Vet. Sci. 26:475–480.
          30. Salminen S, Deighton M, Gorbach S. Lactic acid bacteria in health and disease. p 199–225 In Salminen S, Von Wright A. (ed), Lactic acid bacteria. Marcel Dekker, Inc, New York, NY.
          31. Sambuy Y, De Angelis I, Ranaldi G, Scarino ML, Stammati A, Zucco F. The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics.. Cell Biol Toxicol 2005 Jan;21(1):1-26.
            pubmed: 15868485doi: 10.1007/s10565-005-0085-6google scholar: lookup
          32. Schillinger U, Guigas C, Holzapfel WH. In vitro adherence and other properties of lactobacilli used in probiotic yoghurt-like products. Int. Dairy J. 15:1289–1297.
          33. Strompfová V, Marcináková M, Simonová M, Gancarcíková S, Jonecová Z, Sciranková L, Koscová J, Buleca V, Cobanová K, Lauková A. Enterococcus faecium EK13--an enterocin a-producing strain with probiotic character and its effect in piglets.. Anaerobe 2006 Oct-Dec;12(5-6):242-8.
            pubmed: 17071114doi: 10.1016/j.anaerobe.2006.09.003google scholar: lookup
          34. Tateo A, Valle E, Padalino B, Centoducati P, Bergero D. Change in some physiologic variables induced by Italian traditional conditioning in starndardbred yearling. J. Equine Vet. Sci. 28:743–750.
          35. Tuomola EM, Salminen SJ. Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures.. Int J Food Microbiol 1998 May 5;41(1):45-51.
            pubmed: 9631336doi: 10.1016/s0168-1605(98)00033-6google scholar: lookup
          36. Walter J, Hertel C, Tannock GW, Lis CM, Munro K, Hammes WP. Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis.. Appl Environ Microbiol 2001 Jun;67(6):2578-85.
            pmc: PMC92910pubmed: 11375166doi: 10.1128/aem.67.6.2578-2585.2001google scholar: lookup
          37. Zobba R. Physical, hematological, and biochemical responses to acute intense exercise in polo horses. J. Equine Vet. Sci. 31:542–548.

          Citations

          This article has been cited 8 times.
          1. Lv R, Gao X, Zhang C, Lian W, Quan X, Guo S, Chen X. Characteristics and Whole-Genome Analysis of Limosilactobacillus fermentum Phage LFP02. Foods 2023 Jul 16;12(14).
            doi: 10.3390/foods12142716pubmed: 37509808google scholar: lookup
          2. Ramirez-Sánchez DA, Navarro-Lleó N, Bäuerl C, Campista-León S, Coll-Marqués JM, Pérez-Martínez G. Factors Affecting Spontaneous Endocytosis and Survival of Probiotic Lactobacilli in Human Intestinal Epithelial Cells. Microorganisms 2022 May 31;10(6).
            doi: 10.3390/microorganisms10061142pubmed: 35744660google scholar: lookup
          3. Suzuki S, Fujita K, Maeno S, Shiwa Y, Endo A, Yokota K, Igimi S, Kajikawa A. PCR-based screening, isolation, and partial characterization of motile lactobacilli from various animal feces. BMC Microbiol 2020 Jun 3;20(1):142.
            doi: 10.1186/s12866-020-01830-7pubmed: 32493209google scholar: lookup
          4. Toh H, Nakano A, Nguyen CT, Mimura I, Arakawa K, Tashiro K, Kikusui T, Morita H. Draft Genome Sequence of Coccoid Lactobacillus equigenerosi NRIC 0697T Isolated from the Gastrointestinal Tracts of Healthy Thoroughbreds. Genome Announc 2016 Feb 4;4(1).
            doi: 10.1128/genomeA.01679-15pubmed: 26847890google scholar: lookup
          5. Johnson MB, Criss AK. Fluorescence microscopy methods for determining the viability of bacteria in association with mammalian cells. J Vis Exp 2013 Sep 5;(79).
            doi: 10.3791/50729pubmed: 24056524google scholar: lookup
          6. Fujimoto R, Kuchida M, Ban-Tokuda T, Matsui H. Isolation and molecular identification of Lactobacillaceae bacteria and Bifidobacterium from horse feces. J Equine Sci 2025;36(1):39-43.
            doi: 10.1294/jes.36.39pubmed: 40115730google scholar: lookup
          7. Bishop RC, Kemper AM, Clark LV, Wilkins PA, McCoy AM. Stability of Gastric Fluid and Fecal Microbial Populations in Healthy Horses under Pasture and Stable Conditions. Animals (Basel) 2024 Oct 16;14(20).
            doi: 10.3390/ani14202979pubmed: 39457909google scholar: lookup
          8. Paul LJ, Ericsson AC, Andrews FM, McAdams Z, Keowen ML, St Blanc MP, Banse HE. Field study examining the mucosal microbiome in equine glandular gastric disease. PLoS One 2023;18(12):e0295697.
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