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Home / Equine Research Bank / Int J Syst Evol Microbiol / Lactobacillus hayakitensis sp. nov., isolated from intestine...
International journal of systematic and evolutionary microbiology2007; 57(Pt 12); 2836-2839; doi: 10.1099/ijs.0.65135-0

Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds.

Abstract: Two strains, KBL13(T) and GBL13, were isolated as one of intestinal lactobacilli from the faecal specimens from different thoroughbreds of the same farm where they were born in Hokkaido, Japan. They were Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods. KBL13(T) and GBL13 homofermentatively metabolize glucose, and produce lactate as the sole final product from glucose. The 16S rRNA gene sequence, DNA-DNA hybridization, DNA G+C content and biochemical characterization indicated that these two strains, KBL13(T) and GBL13, belong to the same species. In the representative strain, KBL13(T), the DNA G+C content was 34.3 mol%. Lactobacillus salivarius JCM 1231(T) (=ATCC 11741(T); AF089108) is the type strain most closely related to the strain KBL13(T) as shown in the phylogenetic tree, and the 16S rRNA gene sequence identity showed 96.0 % (1425/1484 bp). Comparative 16S rRNA gene sequence analysis of this strain indicated that the two isolated strains belong to the genus Lactobacillus and that they formed a branch distinct from their closest relatives, L. salivarius, Lactobacillus aviarius, Lactobacillus saerimneri and Lactobacillus acidipiscis. DNA-DNA reassociation experiments with L. salivarius and L. aviarius confirmed that KBL13(T) represents a novel species, for which the name Lactobacillus hayakitensis sp. nov. is proposed. The type strain is KBL13(T) (=JCM 14209(T)=DSM 18933(T)).
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Publication Date: 2007-12-01 PubMed ID: 18048734PubMed Central: PMC2884931DOI: 10.1099/ijs.0.65135-0Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article focuses on identifying a new species of the bacteria Lactobacillus, named Lactobacillus hayakitensis sp. nov., that was isolated from the intestines of healthy thoroughbreds in the same farm in Hokkaido, Japan.

Methodology and Findings

  • The research process began by isolating two strains, KBL13(T) and GBL13, from faecal specimens of different thoroughbreds from the same farm. These strains were identified to be Gram-positive, facultatively anaerobic, catalase-negative, non-spore-forming and non-motile rods.
  • It was observed that both the strains KBL13(T) and GBL13 were capable of metabolizing glucose in an homofermentative manner, i.e., they produce lactate as the sole final product from glucose.
  • The strains were further identified through the 16S rRNA gene sequence, DNA-DNA hybridization, calculation of DNA G+C content and biochemical characterization. It was confirmed that these two strains belong to the same species.
  • Interestingly, the DNA G+C content of the representative strain KBL13(T) was found to be 34.3 mol%.
  • When compared to a standard strain, Lactobacillus salivarius JCM 1231(T) (=ATCC 11741(T); AF089108), it was found that it had the most genetic similarity to the new strain KBL13(T) as depicted by a phylogenetic tree. The similarity in the 16S rRNA gene sequence was noted to be 96.0% (1425/1484 bp).
  • Comparative 16S rRNA gene sequence analysis of this sample demonstrated that the two isolated strains belong to the Lactobacillus genus and formed a distinct branch separate from their closest relatives, which include L. salivarius, Lactobacillus aviarius, Lactobacillus saerimneri and Lactobacillus acidipiscis.
  • Further confirmation of this novel species came from DNA-DNA reassociation experiments conducted with L. salivarius and L. aviarius. These tests unequivocally proved that KBL13(T) represents a novel species, which the researchers proposed to call Lactobacillus hayakitensis sp. nov.

Conclusions

  • This research demonstrated the discovery of a novel species of Lactobacillus, named Lactobacillus hayakitensis sp. nov., that was found in the intestines of healthy thoroughbreds on a farm in Japan.
  • This confirmation was reached through a series of tests and genetic comparisons that included 16S rRNA gene sequencing, DNA-DNA hybridization, DNA G+C content calculation, and biochemical characterization.
  • This discovery contributes to the continued understanding of the diverse species within the Lactobacillus genus and may have potential implications in understanding the gut microflora of horses or potentially be used in probiotic applications.

Cite This Article

APA
Morita H, Shiratori C, Murakami M, Takami H, Kato Y, Endo A, Nakajima F, Takagi M, Akita H, Okada S, Masaoka T. (2007). Lactobacillus hayakitensis sp. nov., isolated from intestines of healthy thoroughbreds. Int J Syst Evol Microbiol, 57(Pt 12), 2836-2839. https://doi.org/10.1099/ijs.0.65135-0

Publication

International journal of systematic and evolutionary microbiology
ISSN: 1466-5026
NlmUniqueID: 100899600
Country: England
Language: English
Volume: 57
Issue: Pt 12
Pages: 2836-2839

Researcher Affiliations

Morita, Hidetoshi
  • School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
Shiratori, Chiharu
  • School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
Murakami, Masaru
  • School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
Takami, Hideto
  • Microbial Genome Research Group, Japan Agency of Marine-Earth Science and Technology, 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan.
Kato, Yukio
  • School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
Endo, Akihito
  • Nodai Culture Collection Center, Department of Brewing, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Nakajima, Fumihiko
  • Northern Farm, 275 Hayakita-genbu, Abira-cho, Yufutsu-gun, Hokkaido 059-1432, Japan.
Takagi, Misako
  • Clossfield-Bio Inc., 1-1-20, Higashi Nihonbashi, Chuo, Tokyo 103-0004, Japan.
Akita, Hiroaki
  • Northern Farm, 275 Hayakita-genbu, Abira-cho, Yufutsu-gun, Hokkaido 059-1432, Japan.
Okada, Sanae
  • Nodai Culture Collection Center, Department of Brewing, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya, Tokyo 156-8502, Japan.
Masaoka, Toshio
  • School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.

MeSH Terms

  • Aerobiosis / physiology
  • Anaerobiosis / physiology
  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • Catalase / analysis
  • DNA / chemistry
  • DNA / genetics
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Feces / microbiology
  • Fermentation
  • Genes, rRNA
  • Glucose / metabolism
  • Horses
  • Japan
  • Lactic Acid / metabolism
  • Lactobacillus / classification
  • Lactobacillus / genetics
  • Lactobacillus / isolation & purification
  • Lactobacillus / metabolism
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Spores, Bacterial / cytology

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Citations

This article has been cited 9 times.
  1. Li XB, Huang XX, Li Q, Li XY, Li JH, Li C, He LJ, Jing HX, Yang KL. Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.. BMC Vet Res 2022 Nov 17;18(1):407.
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  2. Gilroy R, Leng J, Ravi A, Adriaenssens EM, Oren A, Baker D, La Ragione RM, Proudman C, Pallen MJ. Metagenomic investigation of the equine faecal microbiome reveals extensive taxonomic diversity.. PeerJ 2022;10:e13084.
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