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Home / Equine Research Bank / Nucleic Acids Res / Large restriction fragments containing poly-TG are highly po...
Nucleic acids research1990; 18(5); 1129-1132; doi: 10.1093/nar/18.5.1129

Large restriction fragments containing poly-TG are highly polymorphic in a variety of vertebrates.

Abstract: Southern blots of genomic DNA from a variety of species digested by restriction endonucleases having a four-bp specificity, were probed with a bovine genomic clone consisting of seven tandem poly-TG stretches separated by a 29bp linker sequence. Highly variable DNA 'fingerprint' patterns were obtained in chicken, sheep, and horse, moderately variable DNA 'fingerprints' in mouse and man, and a monomorphic pattern in Drosophila. In chicken, horse and man a (TG)10 synthetic oligonucleotide probe gave results identical to those given by the bovine probe. Furthermore, in chicken the DNA fingerprint variation showed typical Mendelian inheritance and differed from the fingerprints obtained with Jeffreys 33.6 and M13 minisatellite probes. Thus, for a variety of vertebrate species, poly-TG-containing probes can uncover useful genetic variation.
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Publication Date: 1990-03-11 PubMed ID: 1969619PubMed Central: PMC330425DOI: 10.1093/nar/18.5.1129Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • Non-P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the genetic variability between different vertebrate species by using a method called Southern blotting on DNA fragments containing poly-TG. These sequences showed different ‘fingerprint’ patterns, offering a potential tool for studying genetic variation in these species.

Methodology

  • The researchers used a process called Southern blotting to explore the DNA of various species. This method involves isolating DNA, fragmenting it using restriction endonucleases (enzymes that cut DNA at specific sequences), and probing these fragments with a specific DNA sequence to visually identify its presence and abundance.
  • The researchers used a specific probe, a bovine genomic clone made up of seven consecutive poly-TG sequences separated by a 29 base pair (bp) sequence. The poly-TG sequences are a repeating pattern of thymine and guanine, two of the four bases that make up DNA.

Findings

  • Different species exhibited different ‘fingerprint’ patterns when the researchers applied the probe to the DNA fragments. These patterns were highly variable in chickens, sheep, and horses, moderately variable in mice and humans, and monomorphic (meaning they had only one form) in Drosophila (a type of fruit fly).
  • In chickens, horses and humans, a synthetic oligonucleotide probe also yielded similar results, which further verified the initial findings.
  • In chickens specifically, the DNA fingerprint variation showed a typical Mendelian inheritance, meaning they followed the principles of inheritance first described by Gregor Mendel, the father of modern genetics.

Implications

  • These findings suggest that poly-TG-containing probes can effectively reveal genetic variation among a variety of vertebrate species. This provides a valuable resource for researchers exploring genetic variability and inheritance patterns in these species.

Cite This Article

APA
Kashi Y, Tikochinsky Y, Genislav E, Iraqi F, Nave A, Beckmann JS, Gruenbaum Y, Soller M. (1990). Large restriction fragments containing poly-TG are highly polymorphic in a variety of vertebrates. Nucleic Acids Res, 18(5), 1129-1132. https://doi.org/10.1093/nar/18.5.1129

Publication

Nucleic acids research
ISSN: 0305-1048
NlmUniqueID: 0411011
Country: England
Language: English
Volume: 18
Issue: 5
Pages: 1129-1132

Researcher Affiliations

Kashi, Y
  • Department of Genetics, Hebrew University, Jerusalem, Israel.
Tikochinsky, Y
    Genislav, E
      Iraqi, F
        Nave, A
          Beckmann, J S
            Gruenbaum, Y
              Soller, M

                MeSH Terms

                • Animals
                • Base Sequence
                • Blotting, Southern
                • Cattle / genetics
                • Chickens / genetics
                • Drosophila / genetics
                • Female
                • Horses / genetics
                • Humans
                • Male
                • Mice
                • Molecular Sequence Data
                • Nucleotide Mapping
                • Plasmids
                • Polydeoxyribonucleotides / genetics
                • Polymorphism, Restriction Fragment Length
                • Sheep / genetics

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                Citations

                This article has been cited 10 times.
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