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The Journal of general virology1992; 73 ( Pt 2); 261-268; doi: 10.1099/0022-1317-73-2-261

Latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and co-cultivation from lymphoid tissues.

Abstract: The polymerase chain reaction (PCR) and co-cultivation were used to identify the lymphoreticular system as the site of latency of equid herpesvirus I (EHV-1). Primers for PCR were designed from aligned nucleotide sequences of the glycoprotein gB genes to amplify the same region of both the EHV-1 and EHV-4 genomes. Subsequent restriction digests using specific enzymes distinguished the amplified fragments of the EHV-1 genome from those of the EHV-4 genome. Ten weeks following an experimental infection of five ponies with EHV-1, latent virus was detected by PCR and recovered by co-cultivation, predominantly from lymphoid tissues draining the respiratory tract. Significantly, latent EHV-1 also persisted in peripheral blood leukocytes (PBL). Latent EHV-4, presumably from a preceding natural infection, was also detected in some tissues, including PBL, from all animals. Of additional interest was the recovery of EHV-1 and -4 only in the presence of the ubiquitous EHV-2.
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Publication Date: 1992-02-01 PubMed ID: 1347078DOI: 10.1099/0022-1317-73-2-261Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on the use of Polymerase Chain Reaction (PCR) and co-cultivation to detect and distinguish latent equid herpesviruses 1 and 4 (EHV-1 and EHV-4) predominantly in lymphoid tissues linked to the respiratory tract.

Methodology

  • The researchers used two main methods in this research – the Polymerase Chain Reaction (PCR) and co-cultivation. Both these methods were used to detect the location of latency of EHV-1 in the lymphoreticular system.
  • The PCR method was designed using primers from aligned nucleotide sequences of the gB genes, which are able to amplify the same region of both the EHV-1 and EHV-4 genomes.
  • A technique called restriction digests was used, employing specific enzymes to differentiate the amplified EHV-1 genome fragments from those of the EHV-4 genome.

Experiments and Findings

  • An experimental infection was conducted on five ponies with EHV-1. Ten weeks later, it was found that the latent virus could be detected by PCR and recovered by co-cultivation, especially from lymphoid tissues flowing from the respiratory tract.
  • Additionally, it was found that latent EHV-1 could also continue to persist inside peripheral blood leukocytes (PBL).
  • Latent EHV-4, which is believed to have come from a previous natural infection, was also detected in several tissues, including the peripheral blood leukocytes (PBL) in all the animals.
  • Interestingly, EHV-1 and EHV-4 were only recovered when the widely present EHV-2 was also present.

Significance of the Research

  • The results of this research provide useful information on the latency of EHV-1 and its detection methods, contributing to the larger field of equine health studies.
  • The discovery of the persistence of latent EHV-1 in PBL could be particularly significant for future research in virus latency and reactivation strategies.
  • The detection of latent EHV-4 alongside EHV-1 raises interesting points for further investigation regarding complex interaction and co-infection dynamics among equine herpesviruses.

Cite This Article

APA
Welch HM, Bridges CG, Lyon AM, Griffiths L, Edington N. (1992). Latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and co-cultivation from lymphoid tissues. J Gen Virol, 73 ( Pt 2), 261-268. https://doi.org/10.1099/0022-1317-73-2-261

Publication

The Journal of general virology
ISSN: 0022-1317
NlmUniqueID: 0077340
Country: England
Language: English
Volume: 73 ( Pt 2)
Pages: 261-268

Researcher Affiliations

Welch, H M
  • Royal Veterinary College, London, U.K.
Bridges, C G
    Lyon, A M
      Griffiths, L
        Edington, N

          MeSH Terms

          • Acute Disease
          • Animals
          • Base Sequence
          • DNA, Viral / analysis
          • DNA, Viral / chemistry
          • Fluorescent Antibody Technique
          • Herpesviridae / genetics
          • Herpesviridae / isolation & purification
          • Herpesviridae Infections / microbiology
          • Herpesviridae Infections / veterinary
          • Herpesvirus 1, Equid / genetics
          • Herpesvirus 1, Equid / isolation & purification
          • Horse Diseases / microbiology
          • Horses
          • Leukocytes / microbiology
          • Lymphoid Tissue / microbiology
          • Molecular Sequence Data
          • Polymerase Chain Reaction
          • Polymorphism, Restriction Fragment Length
          • Respiratory System / microbiology
          • Restriction Mapping

          Citations

          This article has been cited 31 times.
          1. Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar C, Herskin M, Michel V, Miranda Chueca MÁ, Roberts HC, Padalino B, Pasquali P, Spoolder H, Ståhl K, Calvo AV, Viltrop A, Winckler C, Carvelli A, Paillot R, Broglia A, Kohnle L, Baldinelli F, Van der Stede Y. Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus-1. EFSA J 2022 Jan;20(1):e07036.
            doi: 10.2903/j.efsa.2022.7036pubmed: 35035581google scholar: lookup
          2. El-Hage C, Mekuria Z, Dynon K, Hartley C, McBride K, Gilkerson J. Association of Equine Herpesvirus 5 with Mild Respiratory Disease in a Survey of EHV1, -2, -4 and -5 in 407 Australian Horses. Animals (Basel) 2021 Nov 30;11(12).
            doi: 10.3390/ani11123418pubmed: 34944194google scholar: lookup
          3. El Brini Z, Fassi Fihri O, Paillot R, Lotfi C, Amraoui F, El Ouadi H, Dehhaoui M, Colitti B, Alyakine H, Piro M. Seroprevalence of Equine Herpesvirus 1 (EHV-1) and Equine Herpesvirus 4 (EHV-4) in the Northern Moroccan Horse Populations. Animals (Basel) 2021 Sep 29;11(10).
            doi: 10.3390/ani11102851pubmed: 34679874google scholar: lookup
          4. Laval K, Poelaert KCK, Van Cleemput J, Zhao J, Vandekerckhove AP, Gryspeerdt AC, Garré B, van der Meulen K, Baghi HB, Dubale HN, Zarak I, Van Crombrugge E, Nauwynck HJ. The Pathogenesis and Immune Evasive Mechanisms of Equine Herpesvirus Type 1. Front Microbiol 2021;12:662686.
            doi: 10.3389/fmicb.2021.662686pubmed: 33746936google scholar: lookup
          5. Giessler KS, Samoilowa S, Soboll Hussey G, Kiupel M, Matiasek K, Sledge DG, Liesche F, Schlegel J, Fux R, Goehring LS. Viral Load and Cell Tropism During Early Latent Equid Herpesvirus 1 Infection Differ Over Time in Lymphoid and Neural Tissue Samples From Experimentally Infected Horses. Front Vet Sci 2020;7:621.
            doi: 10.3389/fvets.2020.00621pubmed: 33102556google scholar: lookup
          6. Sutton G, Thieulent C, Fortier C, Hue ES, Marcillaud-Pitel C, Pléau A, Deslis A, Guitton E, Paillot R, Pronost S. Identification of a New Equid Herpesvirus 1 DNA Polymerase (ORF30) Genotype with the Isolation of a C(2254)/H(752) Strain in French Horses Showing no Major Impact on the Strain Behaviour. Viruses 2020 Oct 13;12(10).
            doi: 10.3390/v12101160pubmed: 33066315google scholar: lookup
          7. Oladunni FS, Horohov DW, Chambers TM. EHV-1: A Constant Threat to the Horse Industry. Front Microbiol 2019;10:2668.
            doi: 10.3389/fmicb.2019.02668pubmed: 31849857google scholar: lookup
          8. Holz CL, Sledge DG, Kiupel M, Nelli RK, Goehring LS, Soboll Hussey G. Histopathologic Findings Following Experimental Equine Herpesvirus 1 Infection of Horses. Front Vet Sci 2019;6:59.
            doi: 10.3389/fvets.2019.00059pubmed: 30886853google scholar: lookup
          9. Marenzoni ML, Stefanetti V, Danzetta ML, Timoney PJ. Gammaherpesvirus infections in equids: a review. Vet Med (Auckl) 2015;6:91-101.
            doi: 10.2147/VMRR.S39473pubmed: 30155436google scholar: lookup
          10. Tallmadge RL, Žygelytė E, Van de Walle GR, Kristie TM, Felippe MJB. Effect of a Histone Demethylase Inhibitor on Equine Herpesvirus-1 Activity In Vitro. Front Vet Sci 2018;5:34.
            doi: 10.3389/fvets.2018.00034pubmed: 29594155google scholar: lookup
          11. Abdelgawad A, Damiani A, Ho SY, Strauss G, Szentiks CA, East ML, Osterrieder N, Greenwood AD. Zebra Alphaherpesviruses (EHV-1 and EHV-9): Genetic Diversity, Latency and Co-Infections. Viruses 2016 Sep 20;8(9).
            doi: 10.3390/v8090262pubmed: 27657113google scholar: lookup
          12. Taktaz Hafshejani T, Nekoei S, Vazirian B, Doosti A, Khamesipour F, Anyanwu MU. Molecular Detection of Equine Herpesvirus Types 1 and 4 Infection in Healthy Horses in Isfahan Central and Shahrekord Southwest Regions, Iran. Biomed Res Int 2015;2015:917854.
            doi: 10.1155/2015/917854pubmed: 26421307google scholar: lookup
          13. Wilkie GS, Kerr K, Stewart JP, Studdert MJ, Davison AJ. Genome sequences of equid herpesviruses 2 and 5. Genome Announc 2015 Mar 12;3(2).
            doi: 10.1128/genomeA.00119-15pubmed: 25767243google scholar: lookup
          14. Hofmann-Sieber H, Wild J, Fiedler N, Tischer K, von Einem J, Osterrieder N, Hofmann H, Köstler J, Wagner R. Impact of ETIF deletion on safety and immunogenicity of equine herpesvirus type 1-vectored vaccines. J Virol 2010 Nov;84(22):11602-13.
            doi: 10.1128/JVI.00677-10pubmed: 20826695google scholar: lookup
          15. Trapp S, von Einem J, Hofmann H, Köstler J, Wild J, Wagner R, Beer M, Osterrieder N. Potential of equine herpesvirus 1 as a vector for immunization. J Virol 2005 May;79(9):5445-54.
            doi: 10.1128/JVI.79.9.5445-5454.2005pubmed: 15827159google scholar: lookup
          16. Camarda G, Spinetti G, Bernardini G, Mair C, Davis-Poynter N, Capogrossi MC, Napolitano M. The equine herpesvirus 2 E1 open reading frame encodes a functional chemokine receptor. J Virol 1999 Dec;73(12):9843-8.
            doi: 10.1128/JVI.73.12.9843-9848.1999pubmed: 10559296google scholar: lookup
          17. Bartels T, Steinbach F, Hahn G, Ludwig H, Borchers K. In situ study on the pathogenesis and immune reaction of equine herpesvirus type 1 (EHV-1) infections in mice. Immunology 1998 Mar;93(3):329-34.
            doi: 10.1046/j.1365-2567.1998.00451.xpubmed: 9640242google scholar: lookup
          18. Sutton GA, Viel L, Carman PS, Boag BL. Pathogenesis and clinical signs of equine herpesvirus-1 in experimentally infected ponies in vivo. Can J Vet Res 1998 Jan;62(1):49-55.
            pubmed: 9442940
          19. Chesters PM, Allsop R, Purewal A, Edington N. Detection of latency-associated transcripts of equid herpesvirus 1 in equine leukocytes but not in trigeminal ganglia. J Virol 1997 May;71(5):3437-43.
            doi: 10.1128/JVI.71.5.3437-3443.1997pubmed: 9094614google scholar: lookup
          20. Feng X, Thompson YG, Lewis JB, Caughman GB. Expression and function of the equine herpesvirus 1 virion-associated host shutoff homolog. J Virol 1996 Dec;70(12):8710-8.
            doi: 10.1128/JVI.70.12.8710-8718.1996pubmed: 8970998google scholar: lookup
          21. Drummer HE, Reubel GH, Studdert MJ. Equine gammaherpesvirus 2 (EHV2) is latent in B lymphocytes. Arch Virol 1996;141(3-4):495-504.
            doi: 10.1007/BF01718313pubmed: 8645091google scholar: lookup
          22. Belák S, Ballagi-Pordány A. Application of the polymerase chain reaction (PCR) in veterinary diagnostic virology. Vet Res Commun 1993;17(1):55-72.
            doi: 10.1007/BF01839180pubmed: 8396281google scholar: lookup
          23. Crabb BS, MacPherson CM, Reubel GH, Browning GF, Studdert MJ, Drummer HE. A type-specific serological test to distinguish antibodies to equine herpesviruses 4 and 1. Arch Virol 1995;140(2):245-58.
            doi: 10.1007/BF01309860pubmed: 7710353google scholar: lookup
          24. Şahinkesen İ, Bilge-Dağalp S. Development of an In-House ELISA for Serological Detection of Equine Herpesvirus-1/4 Antibodies in Turkish Horses. Animals (Basel) 2025 Aug 27;15(17).
            doi: 10.3390/ani15172523pubmed: 40941318google scholar: lookup
          25. James K, Chappell DE, Craig B, Pariseau C, Wright C, van Harreveld P, Barnum S, Pusterla N. Investigation of Selected Prevalence Factors Associated with EHV-2 and/or EHV-5 Infection in Horses with Acute Onset of Fever and Respiratory Signs. Viruses 2025 Apr 25;17(5).
            doi: 10.3390/v17050612pubmed: 40431624google scholar: lookup
          26. El Brini Z, Cullinane A, Garvey M, Fassi Fihri O, Fellahi S, Amraoui F, Loutfi C, Sebbar G, Paillot R, Piro M. First Molecular and Phylogenetic Characterization of Equine Herpesvirus-1 (EHV-1) and Equine Herpesvirus-4 (EHV-4) in Morocco. Animals (Basel) 2025 Jan 5;15(1).
            doi: 10.3390/ani15010102pubmed: 39795045google scholar: lookup
          27. Hu Y, Zhang SY, Sun WC, Feng YR, Gong HR, Ran DL, Zhang BZ, Liu JH. Breaking Latent Infection: How ORF37/38-Deletion Mutants Offer New Hope against EHV-1 Neuropathogenicity. Viruses 2024 Sep 16;16(9).
            doi: 10.3390/v16091472pubmed: 39339948google scholar: lookup
          28. Maeda M, Abe M, Aoshima K, Kobayashi A, Fukushi H, Kimura T. Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus-1 Immediate-Early Gene. Viruses 2024 Jul 25;16(8).
            doi: 10.3390/v16081195pubmed: 39205169google scholar: lookup
          29. Normand C, Thieulent CJ, Fortier C, Sutton G, Senamaud-Beaufort C, Jourdren L, Blugeon C, Vidalain PO, Pronost S, Hue ES. A Screening Study Identified Decitabine as an Inhibitor of Equid Herpesvirus 4 That Enhances the Innate Antiviral Response. Viruses 2024 May 8;16(5).
            doi: 10.3390/v16050746pubmed: 38793627google scholar: lookup
          30. Saklou N, Pleasant S, Lahmers K, Funk R. Prevalence of Latent Equid Herpesvirus Type 1 in Submandibular Lymph Nodes of Horses in Virginia. Pathogens 2023 Jun 7;12(6).
            doi: 10.3390/pathogens12060813pubmed: 37375503google scholar: lookup
          31. Rimstad E, Hyllseth B. Equine herpesviruses 1 and 4: amplification and differentiation by polymerase chain reaction. Acta Vet Scand 1994;35(3):303-6.
            doi: 10.1186/BF03548336pubmed: 7847200google scholar: lookup
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