LDH and LDH isoenzymes of synovial fluid in the horse.
Abstract: LDH is an intracellular enzyme, which when cells degenerate is released to the extracellular spaces and body fluids. Cells and organs in the mammalian body differ from each other with respect to their LDH isoenzyme patterns. These circumstances have led to the use of LDH isoenzyme determinations in laboratory diagnostic work. In the present investigation total LDH activity and LDH isoenzyme distribution in equine synovial fluid from healthy joints, joints with serous arthritis, osteochondrosis dissecans and arthrosis, were determined. The fluids from the diseased joints differed from normal synovial fluid with respect to total LDH activity, and the different joint diseases each seemed to give rise to a characteristic isoenzyme pattern. In order to examine possible sources of the increased LDH activity and altered isoenzyme patterns, blood plasma, red and white blood cells, synovial membrane and articular cartilage were also studied. It was found that LDH4 and LDH5 were present in high amounts in articular cartilage, and an increase in these isoenzymes was the most characteristic feature in synovial fluid from joints with arthrosis. The results were discussed in view of possible diagnostic value of isoenzyme determinations on synovial fluid. I föreliggande artikel redovisas undersökningar av total LDH-aktivitet och LDHnisoenzym-fördelning i hästsynovia från friska leder, leder med serös artrit, osteochondrosis dissecans och artros. Synovia från de sjuka lederna skilde sig från normal synovia genom förhöjd total LDH-aktivitet, och de olika ledsjukdomarna tycktes var och en karaktäriseras av ett speciellt isoenzym-mönster. För att undersöka möjliga källor till de ökade LDH-aktiviterna och de ändrade isoenzymmönstren, undersöktes också blodplasma, röda och vita blodkroppar, synovialmembran och ledbrosk. LDH och LDH befanns förekomma i höga halter i ledbrosk, och ökningar i halterna av dessa båda isoenzymer var den mest iögonenfallande förändringen i synovia från artrosleder.
Publication Date: 1976-01-01 PubMed ID: 952250PubMed Central: PMC8383969DOI: 10.1186/BF03547926Google Scholar: Lookup
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- Journal Article
Summary
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This research investigates the total activity and distribution of the intracellular enzyme Lactate Dehydrogenase (LDH) and its isoenzymes present in the synovial fluid of horses. The study focuses on how different types of joint diseases affect LDH activity and offers potential diagnostic capabilities through understanding LDH isoenzyme patterns.
Investigation of LDH Activity in Equine Synovial Fluid
- The researchers evaluated the total activity and distribution of LDH and its isoenzymes in equine synovial fluid. Synovial fluid is found in the cavities of synovial joints, which cushions and lubricates the joints during movement.
- The study delved into samples from healthy joints and joints experiencing diseases like serous arthritis, osteochondrosis dissecans, and arthrosis. They discovered that the fluids from the diseased joints exhibited different LDH activity compared to healthy synovial fluid. Furthermore, each joint disease seemed to trigger a unique LDH isoenzyme pattern.
- To further comprehend potential sources contributing to the increased LDH activity and the altered isoenzyme patterns, they examined blood plasma, red and white blood cells, synovial membrane, and articular cartilage.
Findings and Discussion
- It was observed that certain types of LDH, specifically LDH4 and LDH5, were found in substantial amounts within the articular cartilage, tissue involved in forming the structure of synovial joints.
- A distinctive increase in these isoenzymes was a prominent characteristic of synovial fluid from joints with arthrosis, a chronic joint condition causing pain and stiffness.
- The results indicated a potential diagnostic value of LDH isoenzyme determinations in synovial fluid when identifying and distinguishing specific joint diseases owing to the differing isoenzyme patterns observed among them.
Conclusion
- The research offered valuable insights into understanding the role of LDH and its isoenzymes in various joint diseases in horses. By identifying the unique LDH isoenzyme patterns associated with each joint illness, it could pave the way for more precise and earlier diagnosis, improving the prognosis and treatment of these diseases.
Cite This Article
APA
Rejnö S.
(1976).
LDH and LDH isoenzymes of synovial fluid in the horse.
Acta Vet Scand, 17(2), 178-189.
https://doi.org/10.1186/BF03547926 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Arthritis / enzymology
- Arthritis / veterinary
- Horse Diseases / enzymology
- Horses / metabolism
- Isoenzymes
- L-Lactate Dehydrogenase / blood
- L-Lactate Dehydrogenase / metabolism
- Osteochondritis / enzymology
- Osteochondritis / veterinary
- Synovial Fluid / enzymology
- Synovitis / enzymology
- Synovitis / veterinary
References
This article includes 14 references
- Alexandersson R, Nettelbladt E, Sundblad L. Lactic dehydrogenase isoenzymes in arthritic synovial fluid: effect of cortisol.. Acta Rheumatol Scand 1968;14(4):243-52.
- Barthel CH, Duncan JR, Ross RF. Lactic dehydrogenase activity in plasma and synovial fluid of normal and Mycoplasma hyorhinis-infected swine.. Am J Vet Res 1971 Dec;32(12):2011-9.
- COHEN AS. LACTIC DEHYDROGENASE (LDH) AND TRANSAMINASE (GOT) ACTIVITY OF SYNOVIAL FLUID AND SERUM IN RHEUMATIC DISEASE STATES, WITH A NOTE ON SYNOVIAL FLUID LDH ISOZYMES.. Arthritis Rheum 1964 Oct;7:490-501.
- Henry RJ, Cannon DC, Winkelman JW. Clinical Chemistry. Principles and Technics. 2nd Ed. 1974, 815–837.
- Hyldgaard-Jensen JF. Lactate dehydrogenase in pigs. Studies of lactate dehydrogenase isoenzymes in blood and organs. Copenhagen: Munksgaard; 1971.
- Lindy S, Turto H, Uitto J, Vainio K. Origin of synovial fluid lactate dehydrogenase in rheumatoid arthritis.. Clin Chim Acta 1971 Dec;35(2):377-82.
- Rejnö S. Work in progress. .
- . Recommended methods for the determination of four enzymes in blood.. Scand J Clin Lab Invest 1974 Jun;33(4):291-306.
- Strömberg B, Rejnö S. Osteochondrosis in the horse. I. A clinical and radiologic investigation of osteochondritis dissecans of the knee and hock joint.. Acta Radiol Suppl 1978;358:139-52.
- Tushan F, Rodnan GP, Altman M, Robin ED. Anaerobic glycolysis and lactate dehydrogenase (LDH) isoenzymes in articular cartilage.. J Lab Clin Med 1969 Apr;73(4):649-56.
- VESELL ES, OSTERLAND KC, BEARN AG, KUNKEL HG. Isozymes of lactice dehydrogenase; their alterations in arthritic synovial fluid and sera.. J Clin Invest 1962 Nov;41(11):2012-9.
- Veys EM, Wieme RJ. Lactate dehydrogenase in synovial fluid diagnostic evaluation of total activity and isoenzyme patterns.. Ann Rheum Dis 1968 Nov;27(6):569-76.
- Wegner G, Mühlbach R. [Value of paper electrophoresis and the LDH determination as aid in differenciation of knee joint effusions].. Beitr Orthop Traumatol 1971;18(1):21-3.
- Wieme RJ. Agar Gel Electrophoresis. Amsterdam: Elsevier; 1965.
Citations
This article has been cited 2 times.- Walter EL, Spreng D, Schmöckel H, Schawalder P, Tschudi P, Friess AE, Stoffel MH. Distribution of lactate dehydrogenase in healthy and degenerative canine stifle joint cartilage. Histochem Cell Biol 2007 Jul;128(1):7-18.
- Rejnö S. Viscosity of equine synovial fluid. Acta Vet Scand 1976;17(2):169-77.
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