Lectin binding patterns of uterine glands in mares with chronic endometrial degeneration.
Abstract: To evaluate changes of glycoconjugate in uterine glands of endometrial tissues obtained from mares. Methods: adult mares. Methods: Uterine biopsy samples were collected during the breeding season and analyzed histologically for signs of chronic endometrial degeneration. Stage of the estrous cycle was established, using clinical examination and determination of hormonal status. Uterine tissue samples were analyzed, using lectin histochemical and immunohistochemical techniques (estrogen and progesterone receptors). Connective tissues were stained to determine alterations of ground substance in periglandular fibrosis. Results: Of 50 mares, 30 (60%) were classified as normal or having modest alterations, and 20 (40%) were classified as having moderate or severe endometrial degeneration. In normal equine endometrium, several lectins (Helix pomatia agglutinin, Lotus tetragonolobus agglutinin, Ricinus communis I agglutinin, Ulex europaeus agglutinin, and wheat germ agglutinin) bound to glycoconjugates of the luminal epithelium and openings of uterine glands. Lectin binding patterns of cystic dilated glands or fibrotic glands in endometrial samples were remarkably strong, whereas normal surrounding cells remained unstained. Lotus tetragonolobus lectin was not suitable for detecting endometrial alterations. Connective tissues stained with Alcian blue and results of Hale colloidal-iron binding revealed acidic ground substance in periglandular fibrosis. Estrogen and progesterone receptors were evenly distributed in healthy and affected endometrial samples. Conclusions: Glycoconjugate patterns of uterine glands were altered in mares with chronic endometrial degeneration. Therefore, uterine secretions are likely to be altered. These changes are not induced by changes in content of estrogen and progesterone receptors in endometrial tissues.
Publication Date: 2001-06-13 PubMed ID: 11400838DOI: 10.2460/ajvr.2001.62.840Google Scholar: Lookup
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- Journal Article
- Biopsy
- Clinical Examination
- Clinical Pathology
- Clinical Study
- Diagnosis
- Disease Diagnosis
- Disease Etiology
- Endometrium
- Equine Diseases
- Equine Health
- Estrogens
- Glycosaminoglycan
- Histopathology
- Hormones
- Horses
- Immunohistochemistry
- Inflammation
- Laboratory Methods
- Mares
- Progesterone
- Reproduction
- Uterine Health
- Veterinary Medicine
Summary
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This research studied the changes in glycoconjugate patterns in the uterine glands of mares with chronic endometrial degeneration. The researchers collected uterine biopsy samples during the breeding season and analyzed them using lectin histochemical and immunohistochemical techniques. Their findings suggest that glycoconjugate patterns of the uterine glands change in mares suffering from chronic endometrial degeneration, which likely leads to altered uterine secretions and these changes are not induced by changes in the content of estrogen and progesterone receptors in the endometrial tissues.
Research Methodology
- The researchers collected uterine biopsy samples from mares during their breeding season.
- The samples were then analyzed histologically for signs of chronic endometrial degeneration.
- The stage of each mare’s estrous cycle was established through clinical examinations and determinations of hormonal status.
- The researchers utilized lectin histochemical and immunohistochemical techniques to analyze the uterine tissue samples.
- The researchers also studied connective tissues to identify alterations of ground substance in periglandular fibrosis.
Key Findings
- Out of 50 mares that were studied, 60% were classified as normal or having modest alterations, while 40% were identified as having moderate to severe endometrial degeneration.
- In normal equine endometrium, a number of lectins bound to glycoconjugates of the luminal epithelium and openings of uterine glands.
- It was observed that lectin binding patterns of cystic dilated glands or fibrotic glands in endometrial samples were significantly strong, in contrast to the normal surrounding cells.
- Lotus tetragonolobus lectin was found to be unsuitable for detecting endometrial alterations.
- Connective tissues stained with Alcian blue and results of Hale colloidal-iron binding illustrated acidic ground substance in periglandular fibrosis.
- Estrogens and progesterone receptors were evenly distributed in both healthy and affected endometrial samples, indicating these were not the cause of degeneration.
Conclusions
- The research concluded that mares with chronic endometrial degeneration experienced changes in glycoconjugate patterns of their uterine glands.
- This suggested that uterine secretions in these mares are likely altered.
- These changes were noted not to be induced by changes in the content of estrogen and progesterone receptors in the endometrial tissues.
Cite This Article
APA
Walter I, Klein M, Handler J, Aurich JE, Reifinger M, Aurich C.
(2001).
Lectin binding patterns of uterine glands in mares with chronic endometrial degeneration.
Am J Vet Res, 62(6), 840-845.
https://doi.org/10.2460/ajvr.2001.62.840 Publication
Researcher Affiliations
- Institute of Histology and Embryology, University of Veterinary Medicine, Vienna, Austria.
MeSH Terms
- Animals
- Biopsy / veterinary
- Chronic Disease
- Endometrium / metabolism
- Endometrium / pathology
- Estrogens / blood
- Estrus
- Female
- Horse Diseases / metabolism
- Horse Diseases / pathology
- Horses
- Immunohistochemistry / veterinary
- Lectins / metabolism
- Plant Lectins
- Progesterone / blood
- Receptors, Estrogen / metabolism
- Receptors, Progesterone / metabolism
- Uterine Diseases / metabolism
- Uterine Diseases / pathology
- Uterine Diseases / veterinary
Citations
This article has been cited 2 times.- Alpoim-Moreira J, Szóstek-Mioduchowska A, Słyszewska M, Rebordão MR, Skarzynski DJ, Ferreira-Dias G. 5-Aza-2'-Deoxycytidine (5-Aza-dC, Decitabine) Inhibits Collagen Type I and III Expression in TGF-β1-Treated Equine Endometrial Fibroblasts.. Animals (Basel) 2023 Mar 30;13(7).
- Jasiński T, Zdrojkowski Ł, Ferreira-Dias G, Kautz E, Juszczuk-Kubiak E, Domino M. Molecular Mechanism of Equine Endometrosis: The NF-κB-Dependent Pathway Underlies the Ovarian Steroid Receptors' Dysfunction.. Int J Mol Sci 2022 Jul 1;23(13).
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