Leukocyte alkaline phosphatase and serum gamma-glutamil transferase activities in horses used for production of hyper immune sera.

The study examines the reaction of leukocyte alkaline phosphatase (l-AP) and serum gamma-glutamil transferase (gamma-GT) in horses that have been induced to produce hyper immune sera against tetanus. The results depict potential changes to vital health parameters in these horses and assess their health state under such conditions.

Objectives of the Study

• The research aimed to study the changes that occurred in horse leukocytes when they were induced to produce hyper immune sera against tetanus.
• It also sought to evaluate the efficacy of using the counts of leukocyte alkaline phosphatase (l-AP) and serum gamma-glutamil transferase (gamma-GT) as indicators for assessing the health condition of horses under these circumstances.

Research Methodology

• The researchers determined the total leukocyte count, l-AP, and gamma-GT activities of horses submitted to the production of hyper immune sera against tetanus.
• The study concentrated on the evaluation of potential variations within these parameters throughout the duration of the experiment.

Key Findings

• The results showed that the average total leukocyte count increased in one month from the values regarded as physiological to a higher level, where it remained until the completion of the investigation.
• Contrarily, l-AP activities dropped after 30 days of the trial and exhibited a subtle decreasing trend thereafter.
• The serum gamma-GT activity, however, showed a gradual increase throughout the entire experiment.

Implications of the Study

• The findings suggest that these parameters may reflect the dynamics of intensified leukocyte metabolism in the course of chronic inflammation, and the development of associated pathological changes in the liver.
• Additionally, the onset of pathological changes in the organism of horses in the experiment could be between the fourth and sixth months following exposure to the antigen.