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Home / Equine Research Bank / J Clin Microbiol / Lipoproteins as substitutes for serum in Mycoplasma culture ...
Journal of clinical microbiology1979; 10(4); 586-589; doi: 10.1128/jcm.10.4.586-589.1979

Lipoproteins as substitutes for serum in Mycoplasma culture medium.

Abstract: Crude lipoprotein-containing fractions obtained from sera of three different animal species were tested, in combination with bovine serum in Mycoplasma pneumoniae culture medium. All sera yielded at least one lipoprotein-containing component which was considerably more effective in promoting mycoplasma growth than the unfractionated serum sample from which it was derived. The very low activity of certain whole-serum samples tested in this investigation suggests that toxic substances may be present in whole serum which are not contained in the lipoprotein preparations. The greatest activity appeared in the high-density lipoprotein-containing components of bovine and horse sera and the low-density lipoprotein-containing components of human serum. The high degree of growth-supporting activity of these crude lipoprotein-containing serum components suggests that they may be useful as serum substitutes in mycoplasma culture media.
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Publication Date: 1979-10-01 PubMed ID: 231051PubMed Central: PMC273220DOI: 10.1128/jcm.10.4.586-589.1979Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates how fractions of lipoproteins derived from different animal species’ sera could be more effective in promoting mycoplasma growth in culture media than the unprocessed serum, suggesting they could serve as serum substitutes in mycoplasma culture.

Methodology and Results

  • The researchers extracted crude lipoprotein-containing fractions from the sera of three different animal species and combined them with bovine serum in a Mycoplasma pneumoniae culture medium.
  • All sera produced at least one lipoprotein-containing fraction that significantly outperformed the unprocessed serum it was derived from in promoting mycoplasma growth.
  • This difference was so significant that the research team suggested there could be toxic substances in whole serum that aren’t present in the lipoprotein preparations.

Findings and Implications

  • The study discovered that the highest activity levels occurred in high-density lipoprotein-containing components from bovine and horse sera, as well as in low-density lipoprotein-containing components from human serum.
  • These results suggest that, due to their high level of growth-supporting activity, these crude lipoprotein-containing serum components could potentially be used as substitutes for serum in mycoplasma culture media.
  • In addition to their primary findings, the researchers’ notion that there could be toxic substances in whole serum may point to an area worthy of further investigation.

Conclusion

  • This study presents an intriguing alternative to the usage of unprocessed serum in mycoplasma culture media by isolating and using lipoprotein-containing fractions.
  • This finding could not only facilitate growth in these cultures but also potentially reduce the harm posed by potential toxic elements in whole serum.

Cite This Article

APA
Washburn LR, Somerson NL. (1979). Lipoproteins as substitutes for serum in Mycoplasma culture medium. J Clin Microbiol, 10(4), 586-589. https://doi.org/10.1128/jcm.10.4.586-589.1979

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 10
Issue: 4
Pages: 586-589

Researcher Affiliations

Washburn, L R
    Somerson, N L

      MeSH Terms

      • Animals
      • Blood
      • Cattle
      • Culture Media
      • Horses
      • Humans
      • Lipoproteins, HDL
      • Mycoplasma pneumoniae / growth & development
      • Serum Albumin, Bovine
      • Species Specificity

      References

      This article includes 11 references
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      2. Washburn LR, Hughes JH, Somerson NL. Mycoplasma growth factors in bovine serum fraction.. J Bacteriol 1978 Sep;135(3):818-27.
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        pubmed: 173211
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        pubmed: 1252244doi: 10.1016/0006-291x(76)91178-5google scholar: lookup
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        pubmed: 5000981doi: 10.1016/0305-0491(71)90234-3google scholar: lookup
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        pubmed: 5331622

      Citations

      This article has been cited 3 times.
      1. Sprankel L, Vizarraga D, Martín J, Manger S, Meier-Credo J, Marcos M, Julve J, Rotllan N, Scheffer MP, Escolà-Gil JC, Langer JD, Piñol J, Fita I, Frangakis AS. Essential protein P116 extracts cholesterol and other indispensable lipids for Mycoplasmas.. Nat Struct Mol Biol 2023 Mar;30(3):321-329.
        doi: 10.1038/s41594-023-00922-ypubmed: 36782049google scholar: lookup
      2. Johnson JK, Somerson NL. Cholesterol as a limiting factor in the growth of Mycoplasma pneumoniae.. Appl Environ Microbiol 1980 Aug;40(2):391-9.
        doi: 10.1128/aem.40.2.391-399.1980pubmed: 6781407google scholar: lookup
      3. Sasaki T, Shintani M, Kihara K. Utility of egg yolk medium for cultivation of Mycoplasma pneumoniae.. J Clin Microbiol 1983 Nov;18(5):1167-73.
        doi: 10.1128/jcm.18.5.1167-1173.1983pubmed: 6417162google scholar: lookup
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