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Biomaterials2004; 26(17); 3445-3453; doi: 10.1016/j.biomaterials.2004.09.016

Liquid-vapor interfacial tension of blood plasma, serum and purified protein constituents thereof.

Abstract: A systematic study of water-air (liquid-vapor, LV) interfacial tension gamma(lv) of blood plasma and serum derived from four different mammalian species (human, bovine, ovine and equine) reveals nearly identical concentration-dependence (dgamma(lv)/dlnC(B); where C(B) is plasma/serum dilution expressed in v/v concentration units). Comparison of results to a previously-published survey of purified human-blood proteins further reveals that dgamma(lv)/dlnC(B) of plasma and serum is surprisingly similar to that of purified protein constituents. It is thus concluded that any combination of blood-protein constituents will be substantially similar because dgamma(lv)/dlnC(B) of individual proteins are very similar. Experimental results are further interpreted in terms of a recently-developed theory emphasizing the controlling role of water in protein adsorption. Accordingly, the LV interphase saturates with protein adsorbed from bulk solution at a fixed weight-volume concentration ( approximately 436 mg/mL) independent of protein identity or mixture. As a direct consequence, dgamma(lv)/dlnC(B) of purified proteins closely resembles that of mixed solutions and does not depend on the relative proportions of individual proteins comprising a mixture. Thus variations in the plasma proteome between species are not reflected in dgamma(lv)/dlnC(B) nor is serum different from plasma in this regard, despite being depleted of coagulation proteins (e.g. fibrinogen). A comparison of pendant-drop and Wilhelmy-balance tensiometry as tools for assessing protein gamma(lv) shows that measurement conditions employed in the typical Wilhelmy plate approach fails to achieve the steady-state adsorption state that is accessible to pendant-drop tensiometry.
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Publication Date: 2004-12-29 PubMed ID: 15621233DOI: 10.1016/j.biomaterials.2004.09.016Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article in question conducts a comparative study of water-air interfacial tension in the blood plasma and serum obtained from four different mammal species. Their findings indicate that the concentration-dependence of this tension in plasma and serum is almost the same across all species and even comparable to that in purified blood proteins.

Research Methodology

  • The researchers conducted a systematic review of the liquid-vapor (LV), or water-air, interfacial tension (gamma(lv)) in the blood plasma and serum from four different mammalian species: human, bovine (cattle), ovine (sheep), and equine (horse).
  • This tension’s concentration-dependence was expressed as dgamma(lv)/dlnC(B), with C(B) representing the plasma/serum dilution in volume/volume concentration units.
  • These results were then compared to a previous study that studied gamma(lv) in purified human blood proteins.

Key Findings

  • The researchers found that the concentration-dependence (dgamma(lv)/dlnC(B)) of gamma(lv) in plasma and serum is similar across all species. This uniformity implies that the combination of blood-protein constituents does not significantly affect gamma(lv).
  • More surprisingly, the same concentration-dependence is also comparable to purified blood proteins’ values.
  • The study also theorized that the protein’s absorption at the LV interphase is controlled by the water present and reaches saturation at a fixed weight-volume concentration, unaffected by the protein’s identity or mixture.
  • As a result, dgamma(lv)/dlnC(B) doesn’t vary based on the individual proteins in a mixture or the proportions thereof.
  • Unlike expectations, the variations between species in plasma proteome, or the total set of proteins, do not reflect in dgamma(lv)/dlnC(B). The same applies to the comparison between serum, which is free of coagulation proteins like fibrinogen, and plasma.

Research Tools and Techniques

  • The researchers used pendant-drop tensiometry and Wilhelmy-balance tensiometry to measure the gamma(lv).
  • The study concluded that the typical Wilhelmy plate approach didn’t achieve the steady-state adsorption state compared to the pendant-drop tensiometry method.

Cite This Article

APA
Krishnan A, Wilson A, Sturgeon J, Siedlecki CA, Vogler EA. (2004). Liquid-vapor interfacial tension of blood plasma, serum and purified protein constituents thereof. Biomaterials, 26(17), 3445-3453. https://doi.org/10.1016/j.biomaterials.2004.09.016

Publication

Biomaterials
ISSN: 0142-9612
NlmUniqueID: 8100316
Country: Netherlands
Language: English
Volume: 26
Issue: 17
Pages: 3445-3453

Researcher Affiliations

Krishnan, Anandi
  • Department of Bioengineering, The Pennsylvania State University, 103 Steidle Bldg, University Park, PA 16802-5005, USA.
Wilson, Arwen
    Sturgeon, Jacqueline
      Siedlecki, Christopher A
        Vogler, Erwin A

          MeSH Terms

          • Animals
          • Blood Proteins / analysis
          • Blood Proteins / chemistry
          • Cattle
          • Horses
          • Humans
          • Phase Transition
          • Plasma / chemistry
          • Protein Binding
          • Serum / chemistry
          • Sheep
          • Species Specificity
          • Surface Tension

          Grant Funding

          • 5 R01 HL 69965-03 / NHLBI NIH HHS

          Citations

          This article has been cited 16 times.
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