Localization of the horse (Equus caballus) alpha-globin gene complex to chromosome 13 by fluorescence in situ hybridization.
Abstract: The alpha-globin gene complex in Equus caballus has been mapped by fluorescence in situ hybridization to the telomeric region of the long arm of chromosome 13. This is the first equine gene to be mapped to this chromosome.
Publication Date: 1993-01-01 PubMed ID: 8428512DOI: 10.1159/000133456Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research revolves around successfully mapping the alpha-globin gene complex in horses (Equus caballus) to chromosome 13 using fluorescence in situ hybridization. This is a pioneering achievement as no other equine gene has been linked to this chromosome before.
Method of Research: Fluorescence In Situ Hybridization (FISH)
- The researchers used a technique called fluorescence in situ hybridization (FISH). This method is often employed to detect specific DNA sequences on chromosomes.
- FISH uses fluorescent probes that bind to only those parts of the chromosome which possess identical sequence of nucleotides, making it possible to visualize and map specific genes.
- This approach was used to navigate the horse genome and locate the alpha-globin gene complex on chromosome 13.
The Alpha-Globin Gene Complex
- The alpha-globin gene complex is a crucial part of the genetic makeup that controls the production of the alpha-globin component of hemoglobin. Hemoglobin is a protein responsible for carrying oxygen in the blood stream.
- Understanding the location of this gene complex is essential in studying the genetic composition and possibly diagnosing genetic abnormalities in horses.
- Previous studies involving the alpha-globin gene complex have primarily focused on humans and other animals, but its exact location in equine genetic structure was not known prior to this study.
Results and Implications
- The study found that the alpha-globin gene complex is situated on the long arm of chromosome 13 in horses, making this gene the first ever to be mapped to this chromosome in the species.
- This finding contributes to our understanding of the equine genome and expands the potential for future genetic studies in horses.
- Having a clearer picture of the genetic layout in horses can help inform breeding strategies and medical treatments, identify potential genetic disease risks, and assist in the conservation of different horse breeds.
Cite This Article
APA
Oakenfull EA, Buckle VJ, Clegg JB.
(1993).
Localization of the horse (Equus caballus) alpha-globin gene complex to chromosome 13 by fluorescence in situ hybridization.
Cytogenet Cell Genet, 62(2-3), 136-138.
https://doi.org/10.1159/000133456 Publication
Researcher Affiliations
- MRC Molecular Haematology Unit, University of Oxford, UK.
MeSH Terms
- Animals
- Chromosome Mapping
- Globins / genetics
- Horses / genetics
- In Situ Hybridization, Fluorescence
- Karyotyping
- Multigene Family
- Telomere
Citations
This article has been cited 8 times.- Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence.. Chromosome Res 2008;16(1):109-27.
- Hughes JR, Cheng JF, Ventress N, Prabhakar S, Clark K, Anguita E, De Gobbi M, de Jong P, Rubin E, Higgs DR. Annotation of cis-regulatory elements by identification, subclassification, and functional assessment of multispecies conserved sequences.. Proc Natl Acad Sci U S A 2005 Jul 12;102(28):9830-5.
- Lindgren G, Breen M, Godard S, Bowling A, Murray J, Scavone M, Skow L, Sandberg K, Guérin G, Binns M, Ellegren H. Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis.. Chromosome Res 2001;9(1):53-9.
- Lindgren G, Sandberg K, Persson H, Marklund S, Breen M, Sandgren B, Carlstén J, Ellegren H. A primary male autosomal linkage map of the horse genome.. Genome Res 1998 Sep;8(9):951-66.
- Bowling AT, Breen M, Chowdhary BP, Hirota K, Lear T, Millon LV, Ponce de Leon FA, Raudsepp T, Stranzinger G. International system for cytogenetic nomenclature of the domestic horse. Report of the Third International Committee for the Standardization of the domestic horse karyotype, Davis, CA, USA, 1996.. Chromosome Res 1997 Nov;5(7):433-43.
- Raudsepp T, Otte K, Rozell B, Chowdhary BP. FISH mapping of the IGF2 gene in horse and donkey-detection of homoeology with HSA11.. Mamm Genome 1997 Aug;8(8):569-72.
- Breen M, Lindgren G, Binns MM, Norman J, Irvin Z, Bell K, Sandberg K, Ellegren H. Genetical and physical assignments of equine microsatellites--first integration of anchored markers in horse genome mapping.. Mamm Genome 1997 Apr;8(4):267-73.
- Raudsepp T, Frönicke L, Scherthan H, Gustavsson I, Chowdhary BP. Zoo-FISH delineates conserved chromosomal segments in horse and man.. Chromosome Res 1996 Apr;4(3):218-25.
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