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Home / Equine Research Bank / Biochem J / Localization of xanthine dehydrogenase mRNA in horse skeleta...
The Biochemical journal1993; 292 ( Pt 3)(Pt 3); 639-641; doi: 10.1042/bj2920639

Localization of xanthine dehydrogenase mRNA in horse skeletal muscle by in situ hybridization with digoxigenin-labelled probe.

Abstract: In situ hybridization was used to localize xanthine dehydrogenase (XDH) mRNA in horse skeletal muscle. Capillary endothelial cells were found to express XDH, but muscle cells did not give any signal. The digoxigenin-labelled probe was produced by PCR with primers based on the cDNA sequence of mouse XDH and horse lung cDNAs. A 4.3 kb mRNA was detected in a Northern blot.
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Publication Date: 1993-06-15 PubMed ID: 8317993PubMed Central: PMC1134160DOI: 10.1042/bj2920639Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the location of xanthine dehydrogenase (XDH) mRNA in horse skeletal muscle using a technique called in situ hybridization with a digoxigenin-labelled probe. The study found that endothelial cells within capillaries express XDH, but muscle cells do not.

Methodology and Findings

  • The researchers used a technique called in situ hybridization to find out where the XDH mRNA is situated within horse skeletal muscle. In situ hybridization is a type of molecular biology technique that allows researchers to visualize and map the location of specific genes or other genetic material within individual cells or tissue sections.
  • The probe they used for the in situ hybridization was labelled with digoxigenin, which is a steroid commonly used in molecular biology as a marker to visualize the location of specific genes.
  • The digoxigenin-labelled probe was produced using Polymerase Chain Reaction (PCR) with primers based on the cDNA sequence of mouse XDH and horse lung cDNAs. PCR is a commonly used technique in molecular biology to make many copies of a specific DNA segment, while primers are short pieces of single-stranded DNA that are used to start the PCR reaction.
  • The key finding from this study is that in horse skeletal muscle, XDH mRNA is expressed in capillary endothelial cells but not in muscle cells. Capillary endothelial cells form the lining of the blood vessels and are involved in many physiological processes, including the control of blood flow and the exchange of substances between the blood and the surrounding tissues.

Significance of the Research

  • Xanthine dehydrogenase (XDH) is an enzyme that plays a key role in the metabolism of purines, which are essential components of DNA and RNA. Therefore, understanding where XDH mRNA is produced, and thus where the XDH protein is likely to be active, is important for understanding biological function and disease mechanisms.
  • The findings of this study contribute to our knowledge about the distribution of XDH in horse skeletal muscle, which might inform future research on the function and regulation of this enzyme in horses, or indeed in other animals. In addition, since XDH is a target for certain drugs, such as those used to treat gout, these findings might have future implications for veterinary medicine.

Cite This Article

APA
Räsänen LA, Karvonen U, Pösö AR. (1993). Localization of xanthine dehydrogenase mRNA in horse skeletal muscle by in situ hybridization with digoxigenin-labelled probe. Biochem J, 292 ( Pt 3)(Pt 3), 639-641. https://doi.org/10.1042/bj2920639

Publication

The Biochemical journal
ISSN: 0264-6021
NlmUniqueID: 2984726R
Country: England
Language: English
Volume: 292 ( Pt 3)
Issue: Pt 3
Pages: 639-641

Researcher Affiliations

Räsänen, L A
  • Department of Biochemistry, College of Veterinary Medicine, Helsinki, Finland.
Karvonen, U
    Pösö, A R

      MeSH Terms

      • Animals
      • Antisense Elements (Genetics)
      • Base Sequence
      • Blotting, Northern
      • Capillaries
      • DNA
      • Digoxigenin
      • Endothelium, Vascular / enzymology
      • Horses
      • In Situ Hybridization / methods
      • Molecular Sequence Data
      • Muscles / cytology
      • Muscles / enzymology
      • Oligonucleotides, Antisense
      • Polymerase Chain Reaction
      • RNA, Messenger / analysis
      • RNA, Messenger / metabolism
      • Xanthine Dehydrogenase / biosynthesis

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      Citations

      This article has been cited 1 times.
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