FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Cell Transplant / Long-Term Cell Tracking Following Local Injection of Mesench...
Cell transplantation2016; 25(12); 2199-2211; doi: 10.3727/096368916X692104

Long-Term Cell Tracking Following Local Injection of Mesenchymal Stromal Cells in the Equine Model of Induced Tendon Disease.

Abstract: Tendon disease has been treated with multipotent mesenchymal stromal cells (MSCs) in the equine large-animal model with promising success. The aim of this study was to gain more insight into the fate and biodistribution of MSCs after local application into tendon lesions by long-term cell tracking in this large-animal model. Superficial digital flexor tendon lesions were induced in all limbs in six horses and injected with 10106 Molday ION Rhodamine B-labeled MSCs suspended in serum or serum alone. Follow-up was performed using low-field magnetic resonance imaging (MRI), flow cytometry, and histology. Cell tracking based on the hypointense artifacts induced by the superparamagnetic iron oxide (SPIO) labeling agent in MRI as well as based on Rhodamine B fluorescence was feasible. However, Prussian blue staining for assessment of histology was not entirely specific for SPIO. Labeled cells could be traced at their injection site by MRI as well as histology for the whole follow-up period of 24 weeks. Although the numbers of labeled cells within the injected tendon lesions decreased over time, part of the applied cells appeared to remain viable and integrated within the injured tissue. Furthermore, small numbers of labeled cells were identified in peripheral blood within the first 24 h after cell injection and could also be found until week 24 within the contralateral control tendon lesions that had been injected with serum. The present findings unveil details on MSC biodistribution and persistence after their local application, which are of clinical relevance with regard to MSC safety and mechanisms of action.
Get Full Text
Publication Date: 2016-07-07 PubMed ID: 27392888DOI: 10.3727/096368916X692104Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper describes a study investigating how mesenchymal stromal cells (MSCs), a type of stem cell, behave and distribute in the body over time after being injected into a horse’s tendon. The results offer insights on the safety and effectiveness of MSCs in treating tendon disease.

Methodology

  • The research involved six horses, each with induced superficial digital flexor tendon lesions in all limbs.
  • These lesions were injected either with MSCs labeled with Molday ION Rhodamine B, a fluorescence dye, and suspended in serum, or with serum alone as a control.
  • The research team used low-field magnetic resonance imaging (MRI), flow cytometry, and histology to track and monitor the cells in the horses over 24 weeks.

Observations

  • The cells were tracked based on hypointense artifacts seen in the MRI, which were induced by the superparamagnetic iron oxide (SPIO) labeling agent used. This was feasible for cell tracking; however, Prussian blue staining for histology assessment wasn’t entirely specific for SPIO.
  • The MSCs remained visible at the injection site for the entire 24-week follow-up period, both in the MRI scans and the histology. Despite the number of labeled cells decreasing over time, some cells appeared viable and fully integrated with the affected tissue.
  • Small amounts of labeled cells were found in the horse’s peripheral blood within the first 24 hours post-injection, and were detectable until the 24th week in the control tendon lesions that were only injected with serum.

Conclusion

This research provides further insight into the behavior and distribution of MSCs following local injection. The researchers concluded that these findings are significant in understanding the clinical safety and action mechanisms of MSCs, as they appear to remain viable and integrated within the affected tissues for extended periods. Further, they can migrate to other parts of the body, including peripheral blood and contralateral control tendon lesions. Therefore, these findings could have significant implications for the development of stem cell-based treatments for tendon disease in the future.

Cite This Article

APA
Burk J, Berner D, Brehm W, Hillmann A, Horstmeier C, Josten C, Paebst F, Rossi G, Schubert S, Ahrberg AB. (2016). Long-Term Cell Tracking Following Local Injection of Mesenchymal Stromal Cells in the Equine Model of Induced Tendon Disease. Cell Transplant, 25(12), 2199-2211. https://doi.org/10.3727/096368916X692104

Publication

Cell transplantation
ISSN: 1555-3892
NlmUniqueID: 9208854
Country: United States
Language: English
Volume: 25
Issue: 12
Pages: 2199-2211

Researcher Affiliations

Burk, Janina
    Berner, Dagmar
      Brehm, Walter
        Hillmann, Aline
          Horstmeier, Carolin
            Josten, Christoph
              Paebst, Felicitas
                Rossi, Giacomo
                  Schubert, Susanna
                    Ahrberg, Annette B

                      MeSH Terms

                      • Animals
                      • Cell Tracking / methods
                      • Cell- and Tissue-Based Therapy / methods
                      • Female
                      • Ferric Compounds / chemistry
                      • Flow Cytometry
                      • Horses
                      • Magnetic Resonance Imaging
                      • Male
                      • Mesenchymal Stem Cells / cytology
                      • Mesenchymal Stem Cells / physiology
                      • Rhodamines / chemistry
                      • Tendon Injuries / surgery
                      • Tendon Injuries / therapy
                      • Tendons / metabolism
                      • Tendons / pathology

                      Citations

                      This article has been cited 20 times.
                      1. Doll CU, Bohner M, Berner D, Buettner K, Horstmeier C, Winter K, Burk J. Approaches to standardising the magnetic resonance image analysis of equine tendon lesions.. Vet Rec Open 2023 Jun;10(1):e257.
                        doi: 10.1002/vro2.57pubmed: 36846276google scholar: lookup
                      2. Armitage AJ, Miller JM, Sparks TH, Georgiou AE, Reid J. Efficacy of autologous mesenchymal stromal cell treatment for chronic degenerative musculoskeletal conditions in dogs: A retrospective study.. Front Vet Sci 2022;9:1014687.
                        doi: 10.3389/fvets.2022.1014687pubmed: 36713862google scholar: lookup
                      3. Zhang N, Xu L, Song H, Bu C, Kang J, Zhang C, Yang X, Han F. Tracking of Stem Cells from Human Exfoliated Deciduous Teeth Labeled with Molday ION Rhodamine-B during Periodontal Bone Regeneration in Rats.. Int J Stem Cells 2023 Feb 28;16(1):93-107.
                        doi: 10.15283/ijsc21204pubmed: 36042010google scholar: lookup
                      4. Andersen C, Uvebrant K, Mori Y, Aarsvold S, Jacobsen S, Berg LC, Lundgren-Åkerlund E, Lindegaard C. Human integrin α10β1-selected mesenchymal stem cells home to cartilage defects in the rabbit knee and assume a chondrocyte-like phenotype.. Stem Cell Res Ther 2022 May 16;13(1):206.
                        doi: 10.1186/s13287-022-02884-2pubmed: 35578319google scholar: lookup
                      5. Aldrich ED, Cui X, Murphy CA, Lim KS, Hooper GJ, McIlwraith CW, Woodfield TBF. Allogeneic mesenchymal stromal cells for cartilage regeneration: A review of in vitro evaluation, clinical experience, and translational opportunities.. Stem Cells Transl Med 2021 Nov;10(11):1500-1515.
                        doi: 10.1002/sctm.20-0552pubmed: 34387402google scholar: lookup
                      6. Sanchez-Diaz M, Quiñones-Vico MI, Sanabria de la Torre R, Montero-Vílchez T, Sierra-Sánchez A, Molina-Leyva A, Arias-Santiago S. Biodistribution of Mesenchymal Stromal Cells after Administration in Animal Models and Humans: A Systematic Review.. J Clin Med 2021 Jun 29;10(13).
                        doi: 10.3390/jcm10132925pubmed: 34210026google scholar: lookup
                      7. Wagner FC, Reese S, Gerlach K, Böttcher P, Mülling CKW. Cyclic tensile tests of Shetland pony superficial digital flexor tendons (SDFTs) with an optimized cryo-clamp combined with biplanar high-speed fluoroscopy.. BMC Vet Res 2021 Jun 25;17(1):223.
                        doi: 10.1186/s12917-021-02914-wpubmed: 34172051google scholar: lookup
                      8. Bojanic C, To K, Hatoum A, Shea J, Seah KTM, Khan W, Malata CM. Mesenchymal stem cell therapy in hypertrophic and keloid scars.. Cell Tissue Res 2021 Mar;383(3):915-930.
                        doi: 10.1007/s00441-020-03361-zpubmed: 33386995google scholar: lookup
                      9. Ribitsch I, Baptista PM, Lange-Consiglio A, Melotti L, Patruno M, Jenner F, Schnabl-Feichter E, Dutton LC, Connolly DJ, van Steenbeek FG, Dudhia J, Penning LC. Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do.. Front Bioeng Biotechnol 2020;8:972.
                        doi: 10.3389/fbioe.2020.00972pubmed: 32903631google scholar: lookup
                      10. Horstmeier C, Ahrberg AB, Berner D, Burk J, Gittel C, Hillmann A, Offhaus J, Brehm W. In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI.. Stem Cells Int 2019;2019:5670106.
                        doi: 10.1155/2019/5670106pubmed: 31933650google scholar: lookup
                      11. Colbath AC, Dow SW, Hopkins LS, Phillips JN, McIlwraith CW, Goodrich LR. Single and repeated intra-articular injections in the tarsocrural joint with allogeneic and autologous equine bone marrow-derived mesenchymal stem cells are safe, but did not reduce acute inflammation in an experimental interleukin-1β model of synovitis.. Equine Vet J 2020 Jul;52(4):601-612.
                        doi: 10.1111/evj.13222pubmed: 31821594google scholar: lookup
                      12. Hillmann A, Paebst F, Brehm W, Piehler D, Schubert S, Tárnok A, Burk J. A novel direct co-culture assay analyzed by multicolor flow cytometry reveals context- and cell type-specific immunomodulatory effects of equine mesenchymal stromal cells.. PLoS One 2019;14(6):e0218949.
                        doi: 10.1371/journal.pone.0218949pubmed: 31247035google scholar: lookup
                      13. Shojaee A, Parham A. Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges.. Stem Cell Res Ther 2019 Jun 18;10(1):181.
                        doi: 10.1186/s13287-019-1291-0pubmed: 31215490google scholar: lookup
                      14. Wang Y, Li L, Wang T, Zhao L, Feng H, Wang Q, Fan L, Feng X, Xiao W, Feng K. The Efficacy of Near-Infrared Spectroscopy Monitoring in Carotid Endarterectomy: A Prospective, Single-Center, Observational Study.. Cell Transplant 2019 Feb;28(2):170-175.
                        doi: 10.1177/0963689718817760pubmed: 30545240google scholar: lookup
                      15. Grady ST, Britton L, Hinrichs K, Nixon AJ, Watts AE. Persistence of fluorescent nanoparticle-labelled bone marrow mesenchymal stem cells in vitro and after intra-articular injection.. J Tissue Eng Regen Med 2019 Feb;13(2):191-202.
                        doi: 10.1002/term.2781pubmed: 30536848google scholar: lookup
                      16. Roth SP, Schubert S, Scheibe P, Groß C, Brehm W, Burk J. Growth Factor-Mediated Tenogenic Induction of Multipotent Mesenchymal Stromal Cells Is Altered by the Microenvironment of Tendon Matrix.. Cell Transplant 2018 Oct;27(10):1434-1450.
                        doi: 10.1177/0963689718792203pubmed: 30251565google scholar: lookup
                      17. Brandt L, Schubert S, Scheibe P, Brehm W, Franzen J, Gross C, Burk J. Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment.. Int J Mol Sci 2018 Aug 28;19(9).
                        doi: 10.3390/ijms19092549pubmed: 30154348google scholar: lookup
                      18. Winter RL, Seeto WJ, Tian Y, Caldwell FJ, Lipke EA, Wooldridge AA. Growth and function of equine endothelial colony forming cells labeled with semiconductor quantum dots.. BMC Vet Res 2018 Aug 23;14(1):247.
                        doi: 10.1186/s12917-018-1572-3pubmed: 30139355google scholar: lookup
                      19. Ahrberg AB, Horstmeier C, Berner D, Brehm W, Gittel C, Hillmann A, Josten C, Rossi G, Schubert S, Winter K, Burk J. Effects of mesenchymal stromal cells versus serum on tendon healing in a controlled experimental trial in an equine model.. BMC Musculoskelet Disord 2018 Jul 18;19(1):230.
                        doi: 10.1186/s12891-018-2163-ypubmed: 30021608google scholar: lookup
                      20. Geburek F, Roggel F, van Schie HTM, Beineke A, Estrada R, Weber K, Hellige M, Rohn K, Jagodzinski M, Welke B, Hurschler C, Conrad S, Skutella T, van de Lest C, van Weeren R, Stadler PM. Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial.. Stem Cell Res Ther 2017 Jun 5;8(1):129.
                        doi: 10.1186/s13287-017-0564-8pubmed: 28583184google scholar: lookup
                      Subscribe to our newsletter
                      Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.