FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Blut / [Long-term therapy using horse anti-dog lymphocyte globulin ...
Blut1977; 34(4); 305-316; doi: 10.1007/BF00996207

[Long-term therapy using horse anti-dog lymphocyte globulin without sensitization against horse protein].

Abstract: Eight mongrel dogs received a standard daily i.v. infusion of 20 mg/kg b.w. deaggregated horse-anti-dog-lymphocyte-globulin (ALG) and additional prednisolone (1 mg/kg b.w. daily i.v.) over a maximum period of 82 days following pretreatment with deaggregated normal horse IgG. No sensitization against horse protein was observed during therapy of afterwards as proved by lack of humoral antibodies against horse antigens, maintained lymphopenia, good compatibility, longterm prolongation of xenogeneic skin graft survival (85.6+/-20.6 days, n=8' untreated controls 12.5+/-1.3 days, n=4) and longterm suppression of cytotoxic antibodies against donor lymphocytes. The level of preformed agglutinating antibodies against horse erythrocytes was significantly reduced, while preformed antibodies against other species remained normal. The immune response to a challenge injection of anti-lymphocyte-serum (ALS) 6-11 weeks after termination of treatment was significantly lower in the ALG treated animals as compared to the control group. These results suggest the involvement of a specific mechanism of unresponsiveness against ALG other than immunosuppression only. It is concluded, that by the described method sensitization against ALG can be prevented during longterm treatment.
Get Full Text
Publication Date: 1977-04-01 PubMed ID: 322771DOI: 10.1007/BF00996207Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • English Abstract
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article shows that long-term treatment of dogs with horse-derived anti-lymphocyte antibiotics, in combination with prednisolone, does not cause an adverse immune response against horse proteins. It also indicates that this treatment suppresses the dogs’ immune responses, extending the survival of transplanted skin grafts.

Study Participants and Treatment

  • The study involved eight mongrel dogs which were treated with deaggregated horse-anti-dog-lymphocyte-globulin (ALG) and prednisolone.
  • The dogs received a standard daily dose of 20 mg/kg b.w. ALG and 1 mg/kg b.w. prednisolone for a maximum of 82 days, after being pretreated with normal horse IgG.

Findings and Indications

  • There was no observed sensitization against horse proteins during treatment or afterward. This was confirmed by the absence of antibodies against horse antigens, sustained lymphopenia, and favorable compatibility.
  • The study noted a long-term prolongation in xenogeneic skin graft survival among treated dogs (85.6+/-20.6 days, n=8), compared to untreated controls (12.5+/-1.3 days, n=4).
  • The treatment also led to long-term suppression of cytotoxic antibodies against donor lymphocytes.
  • The level of preformed antibodies against horse erythrocytes was significantly reduced, while antibodies against other species remained normal.
  • The immune response to a subsequent injection of anti-lymphocyte-serum (ALS) was significantly lower in ALG-treated animals, compared to controls, suggesting that a specific mechanism other than general immunosuppression may be involved.

Conclusion

  • The study concludes that the treatment protocol followed can prevent sensitization against ALG during prolonged treatment.
  • This finding could potentially open up new possibilities for sustained immunosuppression therapy in veterinary medicine, and possibly also for human medicine.

Cite This Article

APA
Scheel J, Duswald KH, Ring J, Seifert J, Scholz S, Brendel W. (1977). [Long-term therapy using horse anti-dog lymphocyte globulin without sensitization against horse protein]. Blut, 34(4), 305-316. https://doi.org/10.1007/BF00996207

Publication

Blut
ISSN: 0006-5242
NlmUniqueID: 0173401
Country: Germany
Language: ger
Volume: 34
Issue: 4
Pages: 305-316

Researcher Affiliations

Scheel, J
    Duswald, K H
      Ring, J
        Seifert, J
          Scholz, S
            Brendel, W

              MeSH Terms

              • Animals
              • Antilymphocyte Serum / administration & dosage
              • Carnivora
              • Dogs
              • Drug Hypersensitivity
              • Female
              • Horses / immunology
              • Immunoglobulin G / administration & dosage
              • Lymphopenia
              • Male
              • Prednisolone / administration & dosage
              • Skin Transplantation
              • Time Factors
              • Transplantation, Heterologous

              References

              This article includes 26 references
              1. Ring J, Seifert J, Lob G, Land W, Coulin K, Brendel W. [The risk of ALG therapy possible side effects: prevention and treatment (author's transl)].. Klin Wochenschr 1973 May 15;51(10):487-93.
                pubmed: 4795666doi: 10.1007/BF01467826google scholar: lookup
              2. Gewurz H, Moberg AW, Johnson D, Simmons RL, Najarian JS. Induction of tolerance to horse gamma globulin (HoGG) and antilymphocyte globulin (HoALG) in humans and experimental animals.. Transplant Proc 1971 Mar;3(1):737-40.
                pubmed: 4106338
              3. Albert ED, Erickson VM, Graham TC, Parr M, Templeton JW, Mickey MR, Thomas ED, Storb R. Serology and genetics of the DL-A system. I. Establishment of specificities.. Tissue Antigens 1973;3(6):417-30.
                pubmed: 4272899doi: 10.1111/j.1399-0039.1973.tb00512.xgoogle scholar: lookup
              4. Lance EM, Kremer M, Abbosh J, Jones VE, Knight S, Medawar PB. Intensive immunosuppression in patients with disseminated sclerosis. I. Clinical response.. Clin Exp Immunol 1975 Jul;21(1):1-12.
                pubmed: 1237376
              5. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. II.. Prog Allergy 1962;6:30-154.
                pubmed: 14482809doi: 10.1159/000313795google scholar: lookup
              6. Pinto M, Brent L, Thomas AV. Specific unresponsiveness to skin allografts in mice. 3. Synergistic effect of tissue extracts, Bordetella pertussis, and antilymphocytic serum.. Transplantation 1974 May;17(5):477-86.
                pubmed: 4363833doi: 10.1097/00007890-197405000-00006google scholar: lookup
              7. Levey RH, Medawar PB. Further experiments on the action of antilymphocytic antiserum.. Proc Natl Acad Sci U S A 1967 Aug;58(2):470-7.
                pubmed: 4860751doi: 10.1073/pnas.58.2.470google scholar: lookup
              8. Brendel W, Land W. [Surprising results of intravenous therapy using antilymphocyte serum in organ transplantations].. Dtsch Med Wochenschr 1968 Nov 29;93(48):2309-13.
                pubmed: 4880266doi: 10.1055/s-0028-1110931google scholar: lookup
              9. MITCHISON NA. INDUCTION OF IMMUNOLOGICAL PARALYSIS IN TWO ZONES OF DOSAGE.. Proc R Soc Lond B Biol Sci 1964 Dec 15;161:275-92.
                pubmed: 14224412doi: 10.1098/rspb.1964.0093google scholar: lookup
              10. Hopf U. [Immunological tolerance].. Klin Wochenschr 1971 Feb 15;49(4):177-93.
                pubmed: 5543069doi: 10.1007/BF01495980google scholar: lookup
              11. Zink R, Seifert J, Ring J, Brendel W. Cumulation and elimination of horse-anti-dog lymphocyte and normal horse gammaglobulin in dogs.. Res Exp Med (Berl) 1976 Jul 2;167(3):231-8.
                pubmed: 62375doi: 10.1007/BF01851647google scholar: lookup
              12. Land W, Seifert J, Fateh-Moghadam A, Hopf U, Brendel W. [Induction of immunological tolerance to horse serum IgG in adult dogs].. Z Gesamte Exp Med Einschl Exp Chir 1969;151(2):117-37.
                pubmed: 4982478
              13. Hopf U, Fateh-Moghadam A, Land W, Brendel W. [Serologic studies of patients under treatment with xenogenous antilymphocyte globulin].. Klin Wochenschr 1970 Aug 1;48(15):906-13.
                pubmed: 4996980doi: 10.1007/BF01487629google scholar: lookup
              14. Lance EM, Medawar PB. Immunosuppressive effects of heterologous antilymphocyte serum in monkeys.. Lancet 1970 Jan 24;1(7639):167-70.
                pubmed: 4189240doi: 10.1016/s0140-6736(70)90407-1google scholar: lookup
              15. Weksler ME, Bull G, Schwartz GH, Stenzel KH, Rubin AL. Induction of tolerance to antilymphocyte globulin in man.. Transplant Proc 1971 Mar;3(1):754-7.
                pubmed: 4937967
              16. Brendel W, Seifert J, Ring J. [The clinical use of antilymphocyte globulin (ALG) (author's transl)].. MMW Munch Med Wochenschr 1975 Aug 29;117(35):1361-8.
                pubmed: 809676
              17. Kashiwagi N, Brantigan CO, Brettschneider L, Groth CG, Starzl TE. Clinical reactions and serologic changes after the administration of heterologous antilymphocyte globulin to human recipients of renal homografts.. Ann Intern Med 1968 Feb;68(2):275-86.
                pubmed: 4181614doi: 10.7326/0003-4819-68-2-275google scholar: lookup
              18. Duswald KH, Scheel Jv, Hammer C, Brendel W. [Long-term graft survival in the xenogeneic system wolf-dog (author's transl)].. Res Exp Med (Berl) 1976 Jul 2;167(3):255-66.
                pubmed: 790497doi: 10.1007/BF01851649google scholar: lookup
              19. Immons RL, Ozerkis AJ, Hoehn RJ. Antiserum to lymphocytes: interactions with chemical immunosuppressants.. Science 1968 Jun 7;160(3832):1127-30.
                pubmed: 4869606doi: 10.1126/science.160.3832.1127google scholar: lookup
              20. Perper R, Pultavituma A, Palutke M, Rosenberg JC. A reversible acute reaction following intravenous administration of antithymocyte globulin.. Int Arch Allergy Appl Immunol 1971;41(6):920-31.
                pubmed: 4130683doi: 10.1159/000230583google scholar: lookup
              21. Seifert J, Ring J. [Incompatibility reactions following foreign serum therapy and their prevention].. Dtsch Med Wochenschr 1975 May 9;100(19):1078-87.
                pubmed: 1092538doi: 10.1055/s-0028-1106341google scholar: lookup
              22. Pichlmayr R. [Preparation and effect of heterologous anti-dog lymphocyte serums].. Z Gesamte Exp Med Einschl Exp Chir 1967;143(2):161-226.
                pubmed: 5584706
              23. Stockman GD, Judd KP, Trentin JJ. Cyclophosphamide-induced tolerance to equine -globulin and to equine antimouse thymocyte globulin in adult mice. II. Resulting prolongation of cardiac allografts in ATG-treated animals.. Transplantation 1972 Feb;13(2):66-71.
                pubmed: 4110383doi: 10.1097/00007890-197202000-00002google scholar: lookup
              24. Cooley DA, Nora JJ, Trentin JJ, Hallman GL, Bloodwell RD, Leachman RD. Clinical experience in transplantation of the human heart: report of ten cases.. Transplant Proc 1969 Jun;1(2):703-12.
                pubmed: 4939667
              25. Denman AM. Anti-lymphocytic antibody and autoimmune disease: a review.. Clin Exp Immunol 1969 Sep;5(3):217-49.
                pubmed: 4980325
              26. James K. The preparation and properties of anti-lymphocytic sera.. Prog Surg 1969;7:140-217.
                pubmed: 4183661doi: 10.1159/000386301google scholar: lookup

              Citations

              This article has been cited 0 times.
              Subscribe to our newsletter
              Join 99,001 horse owners like you who receive our email updates on horse health and nutrition.