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Blut1977; 34(4); 305-316; doi: 10.1007/BF00996207

[Long-term therapy using horse anti-dog lymphocyte globulin without sensitization against horse protein].

Abstract: Eight mongrel dogs received a standard daily i.v. infusion of 20 mg/kg b.w. deaggregated horse-anti-dog-lymphocyte-globulin (ALG) and additional prednisolone (1 mg/kg b.w. daily i.v.) over a maximum period of 82 days following pretreatment with deaggregated normal horse IgG. No sensitization against horse protein was observed during therapy of afterwards as proved by lack of humoral antibodies against horse antigens, maintained lymphopenia, good compatibility, longterm prolongation of xenogeneic skin graft survival (85.6+/-20.6 days, n=8' untreated controls 12.5+/-1.3 days, n=4) and longterm suppression of cytotoxic antibodies against donor lymphocytes. The level of preformed agglutinating antibodies against horse erythrocytes was significantly reduced, while preformed antibodies against other species remained normal. The immune response to a challenge injection of anti-lymphocyte-serum (ALS) 6-11 weeks after termination of treatment was significantly lower in the ALG treated animals as compared to the control group. These results suggest the involvement of a specific mechanism of unresponsiveness against ALG other than immunosuppression only. It is concluded, that by the described method sensitization against ALG can be prevented during longterm treatment.
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Publication Date: 1977-04-01 PubMed ID: 322771DOI: 10.1007/BF00996207Google Scholar: Lookup
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  • English Abstract
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article shows that long-term treatment of dogs with horse-derived anti-lymphocyte antibiotics, in combination with prednisolone, does not cause an adverse immune response against horse proteins. It also indicates that this treatment suppresses the dogs’ immune responses, extending the survival of transplanted skin grafts.

Study Participants and Treatment

  • The study involved eight mongrel dogs which were treated with deaggregated horse-anti-dog-lymphocyte-globulin (ALG) and prednisolone.
  • The dogs received a standard daily dose of 20 mg/kg b.w. ALG and 1 mg/kg b.w. prednisolone for a maximum of 82 days, after being pretreated with normal horse IgG.

Findings and Indications

  • There was no observed sensitization against horse proteins during treatment or afterward. This was confirmed by the absence of antibodies against horse antigens, sustained lymphopenia, and favorable compatibility.
  • The study noted a long-term prolongation in xenogeneic skin graft survival among treated dogs (85.6+/-20.6 days, n=8), compared to untreated controls (12.5+/-1.3 days, n=4).
  • The treatment also led to long-term suppression of cytotoxic antibodies against donor lymphocytes.
  • The level of preformed antibodies against horse erythrocytes was significantly reduced, while antibodies against other species remained normal.
  • The immune response to a subsequent injection of anti-lymphocyte-serum (ALS) was significantly lower in ALG-treated animals, compared to controls, suggesting that a specific mechanism other than general immunosuppression may be involved.

Conclusion

  • The study concludes that the treatment protocol followed can prevent sensitization against ALG during prolonged treatment.
  • This finding could potentially open up new possibilities for sustained immunosuppression therapy in veterinary medicine, and possibly also for human medicine.

Cite This Article

APA
Scheel J, Duswald KH, Ring J, Seifert J, Scholz S, Brendel W. (1977). [Long-term therapy using horse anti-dog lymphocyte globulin without sensitization against horse protein]. Blut, 34(4), 305-316. https://doi.org/10.1007/BF00996207

Publication

Blut
ISSN: 0006-5242
NlmUniqueID: 0173401
Country: Germany
Language: ger
Volume: 34
Issue: 4
Pages: 305-316

Researcher Affiliations

Scheel, J
    Duswald, K H
      Ring, J
        Seifert, J
          Scholz, S
            Brendel, W

              MeSH Terms

              • Animals
              • Antilymphocyte Serum / administration & dosage
              • Carnivora
              • Dogs
              • Drug Hypersensitivity
              • Female
              • Horses / immunology
              • Immunoglobulin G / administration & dosage
              • Lymphopenia
              • Male
              • Prednisolone / administration & dosage
              • Skin Transplantation
              • Time Factors
              • Transplantation, Heterologous

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              This article has been cited 0 times.
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