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Infection and immunity2005; 73(11); 7259-7266; doi: 10.1128/IAI.73.11.7259-7266.2005

LruA and LruB, novel lipoproteins of pathogenic Leptospira interrogans associated with equine recurrent uveitis.

Abstract: Recurrent uveitis as a sequela to Leptospira infection is the most common infectious cause of blindness and impaired vision of horses worldwide. Leptospiral proteins expressed during prolonged survival in the eyes of horses with lesions of chronic uveitis were identified by screening a phage library of Leptospira interrogans DNA fragments with eye fluids from uveitic horses. Inserts of reactive phages encoded several known leptospiral proteins and two novel putative lipoproteins, LruA and LruB. LruA was intrinsically labeled during incubation of L. interrogans in medium containing [14C]palmitic acid, confirming that it is a lipoprotein. lruA and lruB were detected by Southern blotting in infectious Leptospira interrogans but not in nonpathogenic Leptospira biflexa. Fractionation data from cultured Leptospira indicate that LruA and LruB are localized in the inner membrane. Uveitic eye fluids contained significantly higher levels of immunoglobulin A (IgA) and IgG specific for each protein than did companion sera, indicating strong local antibody responses. Moreover, LruA- and LruB-specific antisera reacted with equine ocular components, suggesting an immunopathogenic role in leptospiral uveitis.
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Publication Date: 2005-10-22 PubMed ID: 16239521PubMed Central: PMC1273856DOI: 10.1128/IAI.73.11.7259-7266.2005Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses new lipoproteins found in Leptospira interrogans, a bacteria responsible for recurrent uveitis in horses. This infection is the leading cause of impaired vision or blindness in horses. These new lipoproteins, LruA and LruB, are thought to contribute to the disease’s progression.

Identifying Leptospiral Proteins Associated with Chronic uveitis

  • The researchers identified proteins produced by the disease-causing bacteria during prolonged survival in infected horses’ eyes. They used a phage library of Leptospira interrogans DNA fragments and eye fluids from affected horses for this task.
  • During their screening, the investigators noticed several known leptospiral proteins and two new putative lipoproteins, named LruA and LruB.
  • Through an intrinsic labeling protocol with [14C]palmitic acid, LruA was confirmed as a lipoprotein.

Detection and Localization of LruA and LruB

  • Both LruA and LruB were detected via Southern blotting in infectious Leptospira interrogans but not in the nonpathogenic Leptospira biflexa, indicating their association with infectious strains.
  • Data from cultured Leptospira suggest that both LruA and LruB are localized within the bacteria’s inner membrane.

Antibody Responses Indicative of LruA and LruB Involvement

  • Horse eye fluids affected by uveitis exhibited significantly higher levels of immunoglobulin A (IgA) and immunoglobulin G (IgG) specific for each protein than found in blood serum, indicating strong local antibody responses against these proteins.
  • The fact that antisera specific for LruA and LruB reacted with ocular components in horses suggests these proteins might play a role in triggering the immune response related to leptospiral uveitis, thereby driving the disease pathology.

The findings in this research article provide new insight into the molecular mechanisms behind Leptospira-caused uveitis in horses and offer potential targets for therapeutic intervention.

Cite This Article

APA
Verma A, Artiushin S, Matsunaga J, Haake DA, Timoney JF. (2005). LruA and LruB, novel lipoproteins of pathogenic Leptospira interrogans associated with equine recurrent uveitis. Infect Immun, 73(11), 7259-7266. https://doi.org/10.1128/IAI.73.11.7259-7266.2005

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 73
Issue: 11
Pages: 7259-7266

Researcher Affiliations

Verma, Ashutosh
  • M. H. Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA.
Artiushin, Sergey
    Matsunaga, James
      Haake, David A
        Timoney, John F

          MeSH Terms

          • Acylation
          • Animals
          • Antibodies, Bacterial / blood
          • Antigens, Bacterial / chemistry
          • Antigens, Bacterial / genetics
          • Antigens, Bacterial / immunology
          • Antigens, Bacterial / metabolism
          • Bacterial Proteins / chemistry
          • Bacterial Proteins / genetics
          • Bacterial Proteins / metabolism
          • Cross Reactions / immunology
          • Eye / immunology
          • Gene Expression Regulation, Bacterial
          • Horse Diseases / immunology
          • Horse Diseases / microbiology
          • Horses / blood
          • Horses / immunology
          • Leptospira interrogans / genetics
          • Leptospira interrogans / metabolism
          • Leptospira interrogans / pathogenicity
          • Lipoproteins / chemistry
          • Lipoproteins / genetics
          • Lipoproteins / metabolism
          • Molecular Sequence Data
          • Protein Transport
          • Uveitis / immunology
          • Uveitis / microbiology
          • Uveitis / veterinary
          • Virulence

          Grant Funding

          • R01 AI034431-08 / NIAID NIH HHS
          • AI-34431 / NIAID NIH HHS
          • R21 AI034431 / NIAID NIH HHS
          • R01 AI034431 / NIAID NIH HHS
          • R29 AI034431 / NIAID NIH HHS

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