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Bulletin of the World Health Organization1973; 48(3); 263-268;

Lyophilized combination pools of enterovirus equine antisera: preparation and test procedures for the identification of field strains of 42 enteroviruses.

Abstract: This paper describes the preparation of 8 dried pools (designated A to H) of sera. Each pool is composed of 10 or 11 of 42 individual enterovirus equine sera and contains 500 antibody units of each serum component per 0.1 ml. Procedures for using the antiserum pools are given, and guidance is provided for interpreting the results of serum neutralization tests in identifying field isolates.
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Publication Date: 1973-01-01 PubMed ID: 4355401PubMed Central: PMC2481070
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper discusses the process of preparing lyophilized (freeze-dried) combinations of equine antisera for identifying different enteroviruses. Antisera from 42 specific enteroviruses are pooled together and rehydrated when needed to test for the presence of these viruses in field isolates.

Preparation of Antisera Pools

The main focus of the research paper is the method used for preparing eight distinct pools of antisera, each containing sera from 10 to 11 different enteroviruses. These pools, identified as A to H, are prepared by lyophilizing, or freeze-drying, the antisera. The process involves:

  • Collecting equine antisera for each of the 42 enteroviruses.
  • Combining specific antisera to create eight distinct pools. Each contains antisera from 10 or 11 different enteroviruses.
  • Lyophilizing the pooled antisera to create a stable, long-lasting form.
  • Ensuring that each 0.1ml unit of the dried pools contains 500 antibody units of each serum component.

Use and Application of Antisera Pools

The study further documents the procedures for rehydrating and using these antisera pools, and offers guidance on interpreting the results from serum neutralization tests. The steps include:

  • Rehydrating the lyophilized antisera pools when required for testing.
  • Using the rehydrated antisera in serum neutralization tests to identify the presence of specific enteroviruses in field isolates.
  • Interpreting the results from these tests. The paper provides guidance for understanding and analyzing the outcomes of serum neutralization tests.

Implications and Applications of Research

This research and its innovative strategy of preparing, storing, and using equine antisera could have several key applications and implications:

  • Improving the identification process for enteroviruses in field isolates, which could lead to better disease surveillance and management.
  • Enhancing the efficiency and reducing the resources needed for testing, as antisera can be prepared in advance and stored until needed.
  • Providing a foundation for developing similar strategies for other types of viruses or disease agents.

Cite This Article

APA
Melnick JL, Rennick V, Hampil B, Schmidt NJ, Ho HH. (1973). Lyophilized combination pools of enterovirus equine antisera: preparation and test procedures for the identification of field strains of 42 enteroviruses. Bull World Health Organ, 48(3), 263-268.

Publication

Bulletin of the World Health Organization
ISSN: 0042-9686
NlmUniqueID: 7507052
Country: Switzerland
Language: English
Volume: 48
Issue: 3
Pages: 263-268

Researcher Affiliations

Melnick, J L
    Rennick, V
      Hampil, B
        Schmidt, N J
          Ho, H H

            MeSH Terms

            • Animals
            • Enterovirus / classification
            • Enterovirus / immunology
            • Freeze Drying
            • Horses / immunology
            • Immune Sera
            • Methods
            • Neutralization Tests
            • World Health Organization

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            This article includes 7 references
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