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Home / Equine Research Bank / Mol Biol Rep / Major histocompatibility complex and other allergy-related c...
Molecular biology reports2012; 40(4); 3333-3340; doi: 10.1007/s11033-012-2408-z

Major histocompatibility complex and other allergy-related candidate genes associated with insect bite hypersensitivity in Icelandic horses.

Abstract: Insect bite hypersensitivity (IBH) is an allergic dermatitis of horses caused by bites of insects. IBH is a multifactorial disease with contribution of genetic and environmental factors. Candidate gene association analysis of IBH was performed in a group of 89 Icelandic horses all born in Iceland and imported to Europe. Horses were classified in IBH-affected and non-affected based on clinical signs and history of recurrent dermatitis, and on the results of an in vitro sulfidoleukotriene (sLT)-release assay with Culicoides nubeculosus and Simulium vittatum extract. Different genetic markers were tested for association with IBH by the Fisher's exact test. The effect of the major histocompatibility complex (MHC) gene region was studied by genotyping five microsatellites spanning the MHC region (COR112, COR113, COR114, UM011 and UMN-JH34-2), and exon 2 polymorphisms of the class II Eqca-DRA gene. Associations with Eqca-DRA and COR113 were identified (p < 0.05). In addition, a panel of 20 single nucleotide polymorphisms (SNPs) in 17 candidate allergy-related genes was tested. During the initial screen, no marker from the panel was significantly (p < 0.05) associated with IBH. Five SNPs associated with IBH at p < 0.10 were therefore used for analysis of combined genotypes. Out of them, SNPs located in the genes coding for the CD14 receptor (CD14), interleukin 23 receptor (IL23R), thymic stromal lymphopoietin (TSLP) and transforming growth factor beta 3 (TGFB3) molecules were associated with IBH as parts of complex genotypes. These results are supported by similar associations and by expression data from different horse populations and from human studies.
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Publication Date: 2012-12-30 PubMed ID: 23275235DOI: 10.1007/s11033-012-2408-zGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the genetic factors associated with Insect Bite Hypersensitivity (IBH), an allergic dermatitis condition caused by insect bites in Icelandic horses. Notably, the researchers identified associations with Major Histocompatibility Complex (MHC) gene region and other genes involved in allergic responses.

Understanding IBH and Genetic Factors

  • Insect Bite Hypersensitivity (IBH) is a kind of allergic dermatitis common in horses, triggered by insect bites. This complex condition is influenced by both environmental and genetic factors. The study concentrated on identifying potential associations with candidate genes.
  • The research involved 89 Icelandic horses, all of which were born in Iceland and then moved to Europe. The horses were categorized as IBH-affected and non-affected based on clinical signs and history of recurrent dermatitis, alongside an in vitro sulfidoleukotriene (sLT)-release assay with extracts of the insects Culicoides nubeculosus and Simulium vittatum.
  • Genetic markers were tested for their potential association with IBH using Fisher’s exact test, a statistical methodology used for analyzing categorical data.

Major Histocompatibility Complex (MHC) and Other Allergy-Related Genes

  • The study paid special attention to the Major Histocompatibility Complex (MHC) gene region, as this part of the genome has a vital role in the immune system. Specifically, five microsatellites spanning the MHC region and exon 2 polymorphisms of the class II Eqca-DRA gene were genotyped.
  • The research revealed associations with the Eqca-DRA and COR113 genes, both of which fall within or close to the MHC region and have known functions in immune responses.
  • Additionally, a panel of 20 single nucleotide polymorphisms (SNPs), which are common variations in the DNA sequence, located in 17 candidate allergy-related genes were tested. Although no marker from this panel was significantly associated with IBH in the first round of testing, further analysis of combined genotypes identified several SNPs associated with this condition.

Complex Genotypes and Allergies

  • The SNPs identified as associated with IBH were located in genes coding for the CD14 receptor, the interleukin 23 receptor, thymic stromal lymphopoietin, and the transforming growth factor-beta 3 molecules—genes which play prominent roles in immune and inflammatory responses.
  • These gene associations were found to exist as part of complex genotypes, suggesting that interactions between multiple genes influence the susceptibility to IBH.
  • Evidence from other horse populations and human studies bolster these findings, highlighting the role that multiple genes and their interactions play in developing allergic conditions like IBH.

Cite This Article

APA
Klumplerova M, Vychodilova L, Bobrova O, Cvanova M, Futas J, Janova E, Vyskocil M, Vrtkova I, Putnova L, Dusek L, Marti E, Horin P. (2012). Major histocompatibility complex and other allergy-related candidate genes associated with insect bite hypersensitivity in Icelandic horses. Mol Biol Rep, 40(4), 3333-3340. https://doi.org/10.1007/s11033-012-2408-z

Publication

Molecular biology reports
ISSN: 1573-4978
NlmUniqueID: 0403234
Country: Netherlands
Language: English
Volume: 40
Issue: 4
Pages: 3333-3340

Researcher Affiliations

Klumplerova, Marie
  • Institute of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1/3, 61242 Brno, Czech Republic.
Vychodilova, Leona
    Bobrova, Olga
      Cvanova, Michaela
        Futas, Jan
          Janova, Eva
            Vyskocil, Mirko
              Vrtkova, Irena
                Putnova, Lenka
                  Dusek, Ladislav
                    Marti, Eliane
                      Horin, Petr

                        MeSH Terms

                        • Animals
                        • Ceratopogonidae / immunology
                        • Ceratopogonidae / pathogenicity
                        • Dermatitis / genetics
                        • Dermatitis / veterinary
                        • Horses / genetics
                        • Horses / immunology
                        • Hypersensitivity / genetics
                        • Hypersensitivity / immunology
                        • Hypersensitivity / veterinary
                        • Iceland
                        • Insect Bites and Stings / genetics
                        • Insect Bites and Stings / immunology
                        • Major Histocompatibility Complex / genetics
                        • Simuliidae / immunology
                        • Simuliidae / pathogenicity

                        References

                        This article includes 33 references
                        1. Vet J. 2008 Sep;177(3):334-44
                          pubmed: 18023376
                        2. Vet Immunol Immunopathol. 2008 Mar 15;122(1-2):65-75
                          pubmed: 18082270
                        3. J Immunol. 2003 Sep 15;171(6):3262-9
                          pubmed: 12960356
                        4. Anim Genet. 2013 Feb;44(1):44-52
                          pubmed: 22582722
                        5. Immunol Rev. 2011 Jul;242(1):233-46
                          pubmed: 21682749
                        6. Int Arch Allergy Immunol. 2007;144(4):325-37
                          pubmed: 17671392
                        7. Equine Vet J. 2006 Jan;38(1):40-6
                          pubmed: 16411585
                        8. Hum Genet. 2007 Sep;122(2):201-6
                          pubmed: 17587057
                        9. Animal. 2008 Mar;2(3):360-5
                          pubmed: 22445037
                        10. Vet Immunol Immunopathol. 2011 Mar 15;140(1-2):63-74
                          pubmed: 21168921
                        11. Immunogenetics. 2009 Jul;61(7):513-27
                          pubmed: 19557406
                        12. Anim Genet. 2010 Dec;41 Suppl 2:150-3
                          pubmed: 21070289
                        13. Immunogenetics. 2012 Mar;64(3):201-8
                          pubmed: 21947540
                        14. Acta Vet Scand. 2006 May 26;48:3
                          pubmed: 16987399
                        15. Int Arch Allergy Immunol. 2007;143(3):177-84
                          pubmed: 17284926
                        16. Cell. 1986 Aug 15;46(4):583-9
                          pubmed: 2873896
                        17. Vet Immunol Immunopathol. 2012 Jun 30;147(3-4):113-26
                          pubmed: 22575371
                        18. Cell Biol Int. 1995 May;19(5):357-71
                          pubmed: 7640653
                        19. Vet Immunol Immunopathol. 2010 Dec 1;138(3):193-7
                          pubmed: 20728225
                        20. Genome Res. 2009 May;19(5):711-22
                          pubmed: 19411596
                        21. Immunogenetics. 1996;43(5):315-7
                          pubmed: 9110935
                        22. Equine Vet J. 1992 Mar;24(2):113-7
                          pubmed: 1582388
                        23. Anim Genet. 1994 Jun;25 Suppl 1:75-80
                          pubmed: 7943987
                        24. J Anim Sci. 2011 May;89(5):1286-93
                          pubmed: 21239662
                        25. Cell Res. 2010 Jan;20(1):62-71
                          pubmed: 19935773
                        26. PLoS One. 2010 Nov 12;5(11):e13894
                          pubmed: 21103062
                        27. Vet Immunol Immunopathol. 2008 Dec 15;126(3-4):351-61
                          pubmed: 18775570
                        28. J Allergy Clin Immunol. 2005 May;115(5):1056-62
                          pubmed: 15867866
                        29. J Allergy Clin Immunol. 2001 Aug;108(2):281-4
                          pubmed: 11496247
                        30. J Leukoc Biol. 2012 Jun;91(6):877-86
                          pubmed: 22442496
                        31. World J Gastroenterol. 2010 Jan 14;16(2):176-83
                          pubmed: 20066736
                        32. J Mol Med (Berl). 2005 Sep;83(9):682-92
                          pubmed: 15902388
                        33. Brief Bioinform. 2008 Jan;9(1):1-13
                          pubmed: 18083722

                        Citations

                        This article has been cited 9 times.
                        1. Simonin EM, Torsteinsdóttir S, Svansson V, Björnsdóttir S, Freer H, Tarsillo J, Wagner B. Early allergen introduction overrides allergy predisposition in offspring of horses with Culicoides hypersensitivity. Front Immunol 2025;16:1654693.
                          doi: 10.3389/fimmu.2025.1654693pubmed: 41194920google scholar: lookup
                        2. Vasoya D, Tzelos T, Benedictus L, Karagianni AE, Pirie S, Marr C, Oddsdóttir C, Fintl C, Connelley T. High-Resolution Genotyping of Expressed Equine MHC Reveals a Highly Complex MHC Structure. Genes (Basel) 2023 Jul 10;14(7).
                          doi: 10.3390/genes14071422pubmed: 37510326google scholar: lookup
                        3. Jaworska J, Ropka-Molik K, Wocławek-Potocka I, Siemieniuch M. Inter- and intrabreed diversity of the major histocompatibility complex (MHC) in primitive and draft horse breeds. PLoS One 2020;15(2):e0228658.
                          doi: 10.1371/journal.pone.0228658pubmed: 32012208google scholar: lookup
                        4. Olomski F, Fettelschoss V, Jonsdottir S, Birkmann K, Thoms F, Marti E, Bachmann MF, Kündig TM, Fettelschoss-Gabriel A. Interleukin 31 in insect bite hypersensitivity-Alleviating clinical symptoms by active vaccination against itch. Allergy 2020 Apr;75(4):862-871.
                          doi: 10.1111/all.14145pubmed: 31816097google scholar: lookup
                        5. Schurink A, da Silva VH, Velie BD, Dibbits BW, Crooijmans RPMA, Franҫois L, Janssens S, Stinckens A, Blott S, Buys N, Lindgren G, Ducro BJ. Copy number variations in Friesian horses and genetic risk factors for insect bite hypersensitivity. BMC Genet 2018 Jul 30;19(1):49.
                          doi: 10.1186/s12863-018-0657-0pubmed: 30060732google scholar: lookup
                        6. Sadeghi R, Moradi-Shahrbabak M, Miraei Ashtiani SR, Miller DC, Antczak DF. MHC haplotype diversity in Persian Arabian horses determined using polymorphic microsatellites. Immunogenetics 2018 May;70(5):305-315.
                          doi: 10.1007/s00251-017-1039-xpubmed: 29170799google scholar: lookup
                        7. Viļuma A, Mikko S, Hahn D, Skow L, Andersson G, Bergström TF. Genomic structure of the horse major histocompatibility complex class II region resolved using PacBio long-read sequencing technology. Sci Rep 2017 Mar 31;7:45518.
                          doi: 10.1038/srep45518pubmed: 28361880google scholar: lookup
                        8. Miller D, Tallmadge RL, Binns M, Zhu B, Mohamoud YA, Ahmed A, Brooks SA, Antczak DF. Polymorphism at expressed DQ and DR loci in five common equine MHC haplotypes. Immunogenetics 2017 Mar;69(3):145-156.
                          doi: 10.1007/s00251-016-0964-4pubmed: 27889800google scholar: lookup
                        9. Velie BD, Shrestha M, Franҫois L, Schurink A, Tesfayonas YG, Stinckens A, Blott S, Ducro BJ, Mikko S, Thomas R, Swinburne JE, Sundqvist M, Eriksson S, Buys N, Lindgren G. Using an Inbred Horse Breed in a High Density Genome-Wide Scan for Genetic Risk Factors of Insect Bite Hypersensitivity (IBH). PLoS One 2016;11(4):e0152966.
                          doi: 10.1371/journal.pone.0152966pubmed: 27070818google scholar: lookup
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