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Journal of equine science2019; 30(1); 13-16; doi: 10.1294/jes.30.13

Markers for oxidative stress in the synovial fluid of Thoroughbred horses with carpal bone fracture.

Abstract: Arthritis is thought to cause oxidative stress in synovial fluid in humans, but there have been few reports in horses. To evaluate oxidative stress in synovial fluid in horses, this study used 19 horses with unilateral fracture of the carpal joint bone. Synovial fluid was collected from the carpal joint on the fracture (arthritis group) and contralateral (control group) sides. Diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) were then measured, and the oxidative stress index (OSI) was calculated. d-ROMs and OSI of the arthritis group were significantly higher than the control group. BAP of the arthritis group was significantly lower than the control group. Thus, this study revealed that oxidative stress develops in the synovial fluid of horses during arthritis.
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Publication Date: 2019-04-03 PubMed ID: 30944542PubMed Central: PMC6445753DOI: 10.1294/jes.30.13Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research looked at evidence of oxidative stress in the joint fluid of horses with a fractured carpal bone, as compared to the unaffected side, using measures of reactive oxygen metabolites and biological antioxidant potential. The results showed that horses with this type of injury exhibit increased levels of oxidative stress.

Research Objective and Methodology

  • This study aimed to examine the presence and degree of oxidative stress in the synovial fluid of horses with a unilateral carpal joint fracture. The presence of oxidative stress in humans with arthritis is already known, but there have been few studies on its occurrence in horses.
  • The researchers used a cohort of 19 horses which had the said injury, using their non-injured side as a control.
  • The synovial fluid, which lubricates and nourishes the joints, was extracted from both the fractured and non-fractured carpal joints of these horses.

Measurements and Findings

  • The researchers then measured two specific markers in this fluid: diacron-reactive oxygen metabolites (d-ROMs), which are by-products of oxidative stress and can damage tissues, and the biological antioxidant potential (BAP), which gauges the fluid’s ability to resist oxidative stress.
  • In addition, they calculated the oxidative stress index (OSI), a ratio that provides a comprehensive view of the balance between oxidation and antioxidation processes.
  • The results showed that both the d-ROMs and OSI levels were significantly higher in the fluid from the fractured joints, indicating increased oxidative stress.
  • Furthermore, the BAP was significantly lower in the arthritis group, suggesting a decreased ability to combat oxidative stress.

Conclusion and Implications

  • This study, therefore, concluded that oxidative stress, evidenced by increased levels of d-ROMs and OSI, and reduced BAP, does occur in the synovial fluid of horses suffering from a unilateral carpal joint fracture.
  • This new understanding of the role of oxidative stress in this type of equine injury could potentially lead to more effective treatments in future, for example by using antioxidants to counter the harmful effects of oxidative stress.

Cite This Article

APA
Tsuzuki N, Kanbayashi Y, Kusano K. (2019). Markers for oxidative stress in the synovial fluid of Thoroughbred horses with carpal bone fracture. J Equine Sci, 30(1), 13-16. https://doi.org/10.1294/jes.30.13

Publication

Journal of equine science
ISSN: 1340-3516
NlmUniqueID: 9503751
Country: Japan
Language: English
Volume: 30
Issue: 1
Pages: 13-16

Researcher Affiliations

Tsuzuki, Nao
  • Obihiro University of Agriculture and Veterinary Medicine, Hokkaido 080-8555, Japan.
Kanbayashi, Yoshinori
  • Racehorse Hospital, Miho Training Center, Japan Racing Association, Ibaraki 300-0493, Japan.
Kusano, Kanichi
  • Racehorse Hospital, Miho Training Center, Japan Racing Association, Ibaraki 300-0493, Japan.

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Citations

This article has been cited 3 times.
  1. Zamudio-Cuevas Y, Martínez-López V, López-Macay A, Montaño-Armendáriz N, Lozada-Pérez CA, Martínez-Flores K, Hernández-Valencia CG, Sánchez-Sánchez R, Gimeno M, Fernández-Torres J. Antiphagocytic Properties of Polygallic Acid with Implications in Gouty Inflammation. Inflammation 2023 Jul 20;.
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  2. Shono S, Gin A, Minowa F, Okubo K, Mochizuki M. The Oxidative Stress Markers of Horses-the Comparison with Other Animals and the Influence of Exercise and Disease. Animals (Basel) 2020 Apr 3;10(4).
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  3. Marković L, Vićić I, Lazarević Macanović M, Francuski Andrić J, Kovačević Filipović M, Radaković M. Degenerative Changes in MCP/MTP Joints of Working Horses Without Lameness: Integrating CT-Based Assessment and Synovial Fluid Biomarkers. Animals (Basel) 2025 Nov 24;15(23).
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