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Home / Equine Research Bank / Ann Rheum Dis / Matrix metalloproteinase activity in equine synovial fluid: ...
Annals of the rheumatic diseases1999; 57(11); 697-699; doi: 10.1136/ard.57.11.697

Matrix metalloproteinase activity in equine synovial fluid: influence of age, osteoarthritis, and osteochondrosis.

Abstract: To investigate the influence of age, osteoarthritis (OA), and osteochondrosis (OC) on the matrix metalloproteinase (MMP) activity in the synovial fluid (SF) of equine joints. Methods: SF was collected from normal and osteoarthritic metacarpophalangeal joints (normal: 14 adult, 28 juvenile; OA: 22 adult). And from normal and osteochondrotic tarsocrural joints (5 months: 11 normal, 8 OC; 11 months: 7 normal, 6 OC). Subsequently, overall MMP activity was measured. Results: The level of active MMPs was almost twofold higher in SF from juvenile horses (age up to 11 months) than in SF from mature animals (4-30 years; p < 0.001). In juvenile horses MMP activity was higher in 5 month old foals than in 11 month old foals (p < 0.01). In adult horses MMP activity was independent of age. In OA joints the activity was nearly twice as high as in normal joints (p < 0.001). In OC joints MMP activity was not significantly different from normal, age matched, control joints. Conclusions: MMP activity in SF from normal adult joints is not related to age. In juvenile joints MMP activity is significantly higher than activity in joints from adult animals. It is hypothesised that the gradual decrease in MMP activity with increasing age reflects the declining metabolic activity resulting from ceasing growth and the accompanying decrease in cartilage remodelling. The increased MMP activity in osteoarthritis joints most likely reflects matrix destruction. In osteochondrosis MMP mediated matrix degradation appears not to be different from normal joints.
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Publication Date: 1999-01-30 PubMed ID: 9924215PubMed Central: PMC1752503DOI: 10.1136/ard.57.11.697Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the activity of matrix metalloproteinase (MMP), an enzyme that breaks down proteins, in the synovial fluid of horses’ joints. It specifically looks at how this activity is affected by the horse’s age and the presence of conditions such as osteoarthritis and osteochondrosis.

Methods

  • The team of researchers collected synovial fluid (SF) from normal and osteoarthritic metacarpophalangeal (part of the leg) joints from both adult and juvenile horses.
  • Additionally, they collected SF from normal and osteochondrotic tarsocrural (ankle) joints from juvenile horses aged 5 months and 11 months.
  • The overall MMP activity within the collected SF was then measured.

Results

  • MMP activity was found to be nearly twice as high in the SF from young horses (up to 11 months old) compared to that from mature horses (4-30 years old).
  • Among these young horses, the researchers noted higher MMP activity in 5-month-old foals compared to 11-month-old ones. However, there was no significant difference in MMP activity among adult horses of varying ages.
  • In joints affected by osteoarthritis, MMP activity was nearly twice as high as in normal, unaffected joints.
  • However, the presence of osteochondrosis did not significantly alter MMP activity compared to normal, similarly-aged control joints.

Conclusions

  • The study concluded that MMP activity in the SF of adult horse joints does not vary with age.
  • In juvenile horse joints, MMP activity is significantly higher than in adult horse joints, denoting a reduction in metabolic activity with age, as indicated by the decrease in MMP activity.
  • The rise in MMP activity in osteoarthritic joints is likely due to the matrix destruction that characterizes this disease.
  • In the case of osteochondrosis, the MMP-mediated matrix degradation is concluded to be no different than in normal joints. This suggests that osteochondrosis does not induce increased MMP activity in the same way that osteoarthritis does.

Cite This Article

APA
Brama PA, TeKoppele JM, Beekman B, van Weeren PR, Barneveld A. (1999). Matrix metalloproteinase activity in equine synovial fluid: influence of age, osteoarthritis, and osteochondrosis. Ann Rheum Dis, 57(11), 697-699. https://doi.org/10.1136/ard.57.11.697

Publication

Annals of the rheumatic diseases
ISSN: 0003-4967
NlmUniqueID: 0372355
Country: England
Language: English
Volume: 57
Issue: 11
Pages: 697-699

Researcher Affiliations

Brama, P A
  • Department of General and Large Animal Surgery, Faculty of Veterinary Medicine, Utrecht, The Netherlands.
TeKoppele, J M
    Beekman, B
      van Weeren, P R
        Barneveld, A

          MeSH Terms

          • Age Factors
          • Animals
          • Biomarkers
          • Fluorometry
          • Horse Diseases / enzymology
          • Horses
          • Metalloendopeptidases / metabolism
          • Osteoarthritis / enzymology
          • Osteoarthritis / veterinary
          • Osteochondritis / enzymology
          • Osteochondritis / veterinary
          • Synovial Fluid / enzymology

          Citations

          This article has been cited 7 times.
          1. Amundson LA, Crenshaw TD. Lessons learned from the hypovitaminosis D kyphotic pig model. J Anim Sci 2020 Aug 18;98(Suppl 1):S52-S57.
            doi: 10.1093/jas/skaa146pubmed: 32810238google scholar: lookup
          2. Braucke AFGV, Frederiksen NL, Berg LC, Aarsvold S, Müller FC, Boesen MP, Lindegaard C. Identification and Quantification of Transient Receptor Potential Vanilloid 1 (TRPV1) in Equine Articular Tissue. Animals (Basel) 2020 Mar 18;10(3).
            doi: 10.3390/ani10030506pubmed: 32197454google scholar: lookup
          3. Boere J, van de Lest CHA, de Grauw JC, Plomp SGM, Libregts SFWM, Arkesteijn GJA, Malda J, Wauben MHM, van Weeren PR. Extracellular vesicles in synovial fluid from juvenile horses: No age-related changes in the quantitative profile. Vet J 2019 Feb;244:91-93.
            doi: 10.1016/j.tvjl.2018.12.010pubmed: 30825901google scholar: lookup
          4. McNulty AL, Leddy HA, Liedtke W, Guilak F. TRPV4 as a therapeutic target for joint diseases. Naunyn Schmiedebergs Arch Pharmacol 2015 Apr;388(4):437-50.
            doi: 10.1007/s00210-014-1078-xpubmed: 25519495google scholar: lookup
          5. Lejeune JP, Serteyn D, Gangl M, Schneider N, Deby-Dupont G, Deberg M, Henrotin Y. Plasma concentrations of a type II collagen-derived peptide and its nitrated form in growing Ardenner sound horses and in horses suffering from juvenile digital degenerative osteoarthropathy. Vet Res Commun 2007 Jul;31(5):591-601.
            doi: 10.1007/s11259-007-3518-2pubmed: 17252318google scholar: lookup
          6. Osawa T, Shinozaki T, Takagishi K. Multivariate analysis of biochemical markers in synovial fluid from the shoulder joint for diagnosis of rotator cuff tears. Rheumatol Int 2005 Aug;25(6):436-41.
            doi: 10.1007/s00296-004-0509-2pubmed: 15378264google scholar: lookup
          7. Gepstein A, Arbel G, Blumenfeld I, Peled M, Livne E. Association of metalloproteinases, tissue inhibitors of matrix metalloproteinases, and proteoglycans with development, aging, and osteoarthritis processes in mouse temporomandibular joint. Histochem Cell Biol 2003 Jul;120(1):23-32.
            doi: 10.1007/s00418-003-0544-1pubmed: 12827373google scholar: lookup
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