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The Cornell veterinarian1984; 74(2); 111-123;

Measurement of diffusion of uncharged molecules in articular cartilage.

Abstract: The diffusion of glucose (180 M.W.), inulin (5,000 M.W.) and dextran (20,000 M.W.) into mature bovine and equine articular cartilage was studied. Concentration profiles were determined using a one-dimensional experimental configuration and the diffusion coefficient and partition coefficient calculated from a theoretical model. Glucose was found to diffuse the fastest, followed by inulin and dextran. The partition coefficient was similarly ordered. The rate of diffusion was found to decrease with increasing diffusion time, indicating a dependence on solute concentration. No time variation was evident in the partition coefficient. In addition, no difference was found between species or between the location sites within the animal joint.
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Publication Date: 1984-04-01 PubMed ID: 6205821
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't
  • Research Support
  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the diffusion rate of different uncharged molecules in the joint (articular) cartilage of mature bovines and horses. The study finds that the diffusion rate varies depending on the molecular weight of the substance and does not depend on the species or the specific location in the joint.

Research Methodology

  • Three substances were used for the study: glucose (180 M.W.), inulin (5,000 M.W.) and dextran (20,000 M.W.). The molecular weight (M.W.) of these substances were varied to understand the impact of molecular size on diffusion.
  • The concentration profiles of these substances in the cartilage were determined using a one-dimensional experimental configuration.
  • The researchers used a theoretical model to calculate the diffusion and partition coefficients, which reveal the speed and extent of diffusion respectively.

Key Findings

  • Glucose – the lightest molecule among the three – was found to diffuse the fastest, followed by medium-weight inulin and heavy dextran. Thus, the speed of diffusion appears to be inversely related to the molecular weight of the substance.
  • The partition coefficient, which indicates how widespread the substances are within the cartilage, was ordered similarly: highest for glucose and lowest for dextran.
  • The rate of diffusion was found to decrease as more molecules entered the cartilage (as diffusion time increased), suggesting a concentration dependence of the diffusion rate.
  • There were no observed temporal changes in the partition coefficient, suggesting it is not influenced by changes in the concentration of the substance in the cartilage.
  • No difference in diffusion or partitioning was observed between bovines and horses, or between different parts of the joint, indicating that these factors do not significantly affect the diffusion of uncharged molecules in joint cartilage.

Cite This Article

APA
Allhands RV, Torzilli PA, Kallfelz FA. (1984). Measurement of diffusion of uncharged molecules in articular cartilage. Cornell Vet, 74(2), 111-123.

Publication

The Cornell veterinarian
ISSN: 0010-8901
NlmUniqueID: 0074245
Country: United States
Language: English
Volume: 74
Issue: 2
Pages: 111-123

Researcher Affiliations

Allhands, R V
    Torzilli, P A
      Kallfelz, F A

        MeSH Terms

        • Animals
        • Cartilage, Articular / metabolism
        • Cattle
        • Dextrans / metabolism
        • Diffusion
        • Glucose / metabolism
        • Horses
        • Inulin / metabolism
        • Molecular Weight
        • Time Factors

        Grant Funding

        • AM05778 / NIADDK NIH HHS
        • AM19849 / NIADDK NIH HHS

        Citations

        This article has been cited 13 times.
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