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Measurement of parathyroid hormone in horses.
Abstract: Measurement of parathyroid hormone (PTH) in horses was performed on plasma samples using 2 immunoradiometric assays: a human intact PTH assay and a rat amino-terminal PTH assay. The assays were validated by assessment of their precision, sensitivity and specificity, and also by evaluating PTH changes in the horse in response to variation in blood ionised calcium. Intra- and inter-assay variance, precision and sensitivity were similar for both human and rat assays; however, the rat assay was slightly more precise and sensitive than the human assay. Both assays detected an increase in PTH levels in the horse when blood ionised calcium was decreased and a decline in PTH concentration with hypercalcaemia. Measurement of PTH concentration in samples from healthy horses with the human assay yielded a mean (+/-s.e.) value of 31.3+/-4.1 pg/ml. When using the rat assay, PTH values were 44.1+/-5.3 pg/ml. Plasma samples held for up to 3 months at -20 degrees C did not show a significant change in PTH concentration. In conclusion, the human intact PTH and the rat amino-terminal assays detected equine PTH and can be used for measurement of this hormone in horses. Quantification of equine PTH using these assays will allow more precise diagnosis of a variety of disorders affecting mineral metabolism in horses.
Publication Date: 1998-12-09 PubMed ID: 9844965DOI: 10.1111/j.2042-3306.1998.tb04522.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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This research paper describes a study investigating the measurement of Parathyroid Hormone (PTH) in horses using two different immunoradiometric assays: a human intact PTH assay and a rat amino-terminal PTH assay. The results show that both tests can accurately measure PTH in horses and could help diagnose disorders affecting mineral metabolism in horses.
Research Methodology
The researchers used two different types of immunoradiometric assays to measure the concentration of Parathyroid Hormone (PTH) in horses:
- Human Intact PTH assay
- Rat Amino Terminal PTH assay
These assays were tested for precision, sensitivity, and specificity. In addition, they observed the changes in PTH in response to variations in blood ionized calcium levels in the horses.
Assay Performance
The performance of both the human intact PTH assay and the rat amino-terminal PTH assay was demonstrated to be comparable in terms of their intra- and inter-assay variance, precision and sensitivity. However, the rat assay showed slightly more precision and sensitivity than the human assay. When the blood ionized calcium was decreased, both assays detected an increase in PTH levels in the horse, and conversely a decline in PTH concentration was noted under the conditions of hypercalcemia.
PTH Concentration in Healthy Horses
The researchers quantified the PTH concentration from samples taken from healthy horses. For the human assay, the average measured value was 31.3 ± 4.1 pg/ml. When using the rat assay, the average PTH value was found to be at 44.1 ± 5.3 pg/ml. It was also observed that PTH concentration in plasma samples held for up to 3 months at -20 degrees Celsius remained unchanged, revealing the stability of the samples under these conditions.
Conclusions
The study concludes that both the human intact PTH and rat amino-terminal assays are effective in detecting equine PTH and can, therefore, be utilized for measurement of this hormone in horses. Using these assays can enhance accurate diagnosis of disorders affecting mineral metabolism in horses, thereby aiding in their management and treatment.
Cite This Article
APA
Estepa JC, Aguilera-Tejero E, Mayer-Valor R, Almadén Y, Felsenfeld AJ, Rodríguez M.
(1998).
Measurement of parathyroid hormone in horses.
Equine Vet J, 30(6), 476-481.
https://doi.org/10.1111/j.2042-3306.1998.tb04522.x Publication
Researcher Affiliations
- Departamento de Medicina y Cirugia Animal, Universidad de Córdoba, Spain.
MeSH Terms
- Animals
- Blood Preservation / adverse effects
- Blood Preservation / veterinary
- Calcium / blood
- Cryopreservation / veterinary
- Female
- Horses / blood
- Humans
- Immunoradiometric Assay / veterinary
- Male
- Parathyroid Hormone / blood
- Rats
- Reference Values
- Reproducibility of Results
- Sensitivity and Specificity
Citations
This article has been cited 2 times.- Fradinho MJ, Mateus L, Bernardes N, Bessa RJB, Caldeira RM, Ferreira-Dias G. Growth patterns, metabolic indicators and osteoarticular status in the Lusitano horse: A longitudinal study. PLoS One 2019;14(7):e0219900.
- Lacitignola L, Luca P, Santovito R, Comite MSD, Crovace A. Nutritional secondary hyperparathyroidism in two ponies. Open Vet J 2018;8(2):149-153.
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