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Measurement of plasma cardiac troponin I concentration by use of a point-of-care analyzer in clinically normal horses and horses with experimentally induced cardiac disease.
Abstract: To compare cardiac troponin I (cTnI) concentrations determined by use of a point-of-care analyzer with values determined by use of a bench-top immunoassay in plasma samples obtained from clinically normal horses with and without experimentally induced cardiac disease, and to establish a reference range for plasma equine cTnI concentration determined by use of the point-of-care analyzer. Methods: 83 clinically normal horses, 6 of which were administered monensin to induce cardiac disease. Methods: A blood sample was collected from each of the 83 clinically normal horses to provide plasma for analysis by use of the point-of-care analyzer; some of the same samples were also analyzed by use of the immunoassay. All 83 samples were used to establish an analyzer-specific reference range for plasma cTnI concentration in clinically normal horses. In 6 horses, blood samples were also collected at various time points after administration of a single dose of monensin (1.0 to 1.5 mg/kg) via nasogastric intubation; plasma cTnI concentration in those samples was assessed by use of both methods. Results: The analyzer-specific reference range for plasma cTnI concentration in clinically normal horses was 0.0 to 0.06 ng/mL. Following monensin treatment in 5 horses, increases in plasma cTnI concentration determined by use of the 2 methods were highly correlated (Pearson correlation, 0.83). Peak analyzer-determined plasma cTnI concentrations in monensin-treated horses ranged from 0.08 to 3.68 ng/mL. Conclusions: In horses with and without experimentally induced cardiac disease, the point-of-care analyzer and bench-top immunoassay provided similar values of plasma cTnI concentration.
Publication Date: 2010-01-02 PubMed ID: 20043781DOI: 10.2460/ajvr.71.1.55Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Cardiac Arrhythmias
- Clinical Examination
- Clinical Pathology
- Clinical Study
- Clinical Symptoms
- Diagnosis
- Diagnostic Technique
- Disease Diagnosis
- Disease Etiology
- Disease Treatment
- Equine Diseases
- Equine Health
- Experimental Methods
- Heart
- Heart Rate
- Horses
- Laboratory Methods
- Plasma
- Veterinary Medicine
- Veterinary Procedure
- Veterinary Research
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research project attempted to establish a reference range for plasma equine cardiac troponin I (cTnI) concentration, using a point-of-care analyzer. The results were compared between healthy horses and those with induced cardiac disease. The results showed a high correlation between the point-of-care analyzer and a standard immunoassay.
Research Methodology
- The study involved 83 clinically healthy horses and 6 horses were administered with monensin to generate artificially induced cardiac disease.
- A blood sample was collected from all 83 horses. These samples were then used in the point-of-care analyzer. Some of these samples were also analyzed with a bench-top immunoassay for comparison.
- All 83 samples were used to create a specific reference range for healthy horse plasma cTnI concentration.
- In 6 horses, blood samples were also collected at various durations after the administration of a single dose of monensin (1.0 to 1.5 mg/kg) via nasogastric intubation. Both the point-of-care analyzer and the immunoassay were used to evaluate the plasma cTnI concentration in these samples.
Research Results
- The specific reference range for healthy horse plasma cTnI concentration, using the point-of-care analyzer, was established to be between 0.0 to 0.06 ng/mL.
- A significant correlation (Pearson correlation, 0.83) was noted between results obtained from the point-of-care analyzer and the immunoassay post administration of monensin.
- The cTnI concentrations in the blood of the horses subjected to monensin treatment ranged from 0.08 to 3.68 ng/mL, as per the point-of-care analyzer.
Research Conclusions
- The research concluded that the point-of-care analyzer and bench-top immunoassay provided similar values of plasma cTnI concentration in horses with both natural and experimentally induced cardiac diseases.
Cite This Article
APA
Kraus MS, Jesty SA, Gelzer AR, Ducharme NG, Mohammed HO, Mitchell LM, Soderholm LV, Divers TJ.
(2010).
Measurement of plasma cardiac troponin I concentration by use of a point-of-care analyzer in clinically normal horses and horses with experimentally induced cardiac disease.
Am J Vet Res, 71(1), 55-59.
https://doi.org/10.2460/ajvr.71.1.55 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. msk16@cornell.edu
MeSH Terms
- Animals
- Female
- Heart Diseases / blood
- Heart Diseases / chemically induced
- Heart Diseases / veterinary
- Horse Diseases / blood
- Horses
- Ionophores / toxicity
- Male
- Monensin / toxicity
- Point-of-Care Systems
- Troponin I / blood
Citations
This article has been cited 14 times.- Garcia Filho SG, de Andrade FSRM, Dos Santos RST, Gonçalves LA, Pereira MAA, de Souza AF, Ambrósio AM, Fantoni DT. Comparison of Hemodynamic Effects of Dobutamine and Ephedrine Infusions in Isoflurane-Anesthetized Horses. Vet Sci 2023 Apr 6;10(4).
- Perera TRW, Skerrett-Byrne DA, Gibb Z, Nixon B, Swegen A. The Future of Biomarkers in Veterinary Medicine: Emerging Approaches and Associated Challenges. Animals (Basel) 2022 Aug 26;12(17).
- Pourmohammad R, Mohri M, Seifi HA, Sardari K. Evaluation of cardiac troponin I, atrial natriuretic peptide and some oxidative/antioxidative biomarkers in the serum and hemolysate of trained Arabian horses after exercise. Iran J Vet Res 2020 Summer;21(3):211-215.
- Gunther-Harrington CT, Arthur R, Estell K, Martinez Lopez B, Sinnott A, Ontiveros E, Varga A, Stern JA. Prospective pre- and post-race evaluation of biochemical, electrophysiologic, and echocardiographic indices in 30 racing thoroughbred horses that received furosemide. BMC Vet Res 2018 Jan 18;14(1):18.
- Luethy D, Slack J, Kraus MS, Gelzer AR, Habecker P, Johnson AL. Third-Degree Atrioventricular Block and Collapse Associated with Eosinophilic Myocarditis in a Horse. J Vet Intern Med 2017 May;31(3):884-889.
- Shields E, Seiden-Long I, Massie S, Passante S, Leguillette R. Analytical validation and establishment of reference intervals for a 'high-sensitivity' cardiac troponin-T assay in horses. BMC Vet Res 2016 Jun 13;12(1):104.
- Marly-Voquer C, Schwarzwald CC, Bettschart-Wolfensberger R. The use of dexmedetomidine continuous rate infusion for horses undergoing transvenous electrical cardioversion--A case series. Can Vet J 2016 Jan;57(1):70-5.
- Raftery AG, Garcia NC, Thompson H, Sutton DG. Arrhythmogenic right ventricular cardiomyopathy secondary to adipose infiltration as a cause of episodic collapse in a horse. Ir Vet J 2015;68:24.
- Van Der Vekens N, Decloedt A, Ven S, De Clercq D, van Loon G. Cardiac troponin I as compared to troponin T for the detection of myocardial damage in horses. J Vet Intern Med 2015 Jan;29(1):348-54.
- Tharwat M, Al-Sobayil F. Influence of the cardiac glycoside digoxin on cardiac troponin I, acid-base and electrolyte balance, and haematobiochemical profiles in healthy donkeys (Equus asinus). BMC Vet Res 2014 Mar 12;10:64.
- Morgan RA, Raftery AG, Cripps P, Senior JM, McGowan CM. The prevalence and nature of cardiac arrhythmias in horses following general anaesthesia and surgery. Acta Vet Scand 2011 Nov 23;53(1):62.
- Foreman JH, Tennent-Brown BS, Oyama MA, Sisson DD. Plasma Cardiac Troponin-I Concentration in Normal Horses and in Horses with Cardiac Abnormalities. Animals (Basel) 2025 Jan 3;15(1).
- Mendoza FJ, Buzon-Cuevas A, Aguilera-Aguilera R, Gonzalez-De Cara CA, De Las Heras A, Perez-Ecija A. Hemodynamic Response to Lipopolysaccharide Infusion and Effect of Meloxicam Administration on Cardiac Function in Donkeys. Animals (Basel) 2024 Dec 18;14(24).
- Rautenbach Y, Parsons SDC, Loots AK, Goddard A, Meyer LCR, Buss PE, Hooijberg EH. Genetic characterization of diagnostic epitopes of cardiac troponin I in African rhinoceros. J Vet Diagn Invest 2025 Mar;37(2):263-271.
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