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Home / Equine Research Bank / J Vet Med Sci / Measurement of plasma endothelin-1 concentration in healthy ...
The Journal of veterinary medical science2018; 81(2); 263-268; doi: 10.1292/jvms.18-0325

Measurement of plasma endothelin-1 concentration in healthy horses and horses with cardiac disease during rest and after exercise.

Abstract: Cardiac biomarkers are important tools for monitoring disease progress and can monitor progression of therapy. Endothelin-1 (ET-1) has been studied for its use as a cardiac biomarker in human and small animal medicine while in horses with cardiac disease it has not been evaluated yet. The objective of the present study was to determine the concentration of plasma ET-1 in healthy horses and compare it with ET-1 concentration in horses with cardiac disease during rest and after exercise. Fifty four horses admitted to the Equine Clinic of Free University of Berlin were used in the present study, of which 15 horses were clinically healthy with no evidence of cardiac disease (Group 1), 22 horses suffered from cardiac disease with normal heart dimensions (Group 2) and 17 horses with cardiac disease and enlarged heart diameters (Group 3). Clinical examination, electrocardiography and echocardiography were performed. Endothelin-1 concentration was determined using ET-1 ELISA kit. The concentration of plasma ET-1 was significantly increased in horses with cardiac disease and normal cardiac dimensions (Group 2) and in horses with cardiac disease and enlargement of the left atrium (Group 3) compared to its concentration in clinically healthy horses (Group 1). In addition, the concentration of plasma ET-1 after exercise was significantly increased in diseased horses compared to its concentration at rest. Detection of ET-1 plasma concentration in horses at rest may be useful for detecting horses with changes in left atrial cardiac dimensions.
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Publication Date: 2018-12-28 PubMed ID: 30606891PubMed Central: PMC6395200DOI: 10.1292/jvms.18-0325Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the concentration of the potential cardiac biomarker, Endothelin-1 (ET-1), in horses with and without cardiac disease at rest and after exercise. The research found ET-1 levels higher in horses with cardiac disease, revealing its potential use in early cardiac disease detection in horses.

Research Design and Purpose

  • The purpose of the study was to determine the concentration of the potential cardiac biomarker, Endothelin-1 (ET-1), in healthy horses and horses with cardiac disease during rest and after exercise.
  • The research seeks to examine if ET-1 can help in early detection, monitoring the progression of cardiac disease in horses, or assessing the effectiveness of therapy—similar to its use in human and small animal medicine.

Study Population and Procedure

  • The study used fifty-four horses that were admitted to the Equine Clinic of the Free University of Berlin. The horses were categorized into three groups: Group 1, which had 15 clinically healthy horses without any cardiac diseases; Group 2, which had 22 horses with cardiac diseases but normal heart dimensions; and Group 3, which had 17 horses with cardiac diseases and enlarged heart diameters.
  • Clinical examination, electrocardiography and echocardiography were carried out on the horses. The ET-1 concentration in the plasma of horses was measured using an ET-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit.

Research Findings

  • The researchers found that the plasma ET-1 concentration was significantly higher in horses with cardiac disease, with normal cardiac dimensions (Group 2) and in horses with an enlarged left atrium (Group 3) compared to the ET-1 concentration in healthy horses (Group 1). This indicates that ET-1 may be a useful biomarker for detecting cardiac disease in horses.
  • Additionally, it was observed that the plasma ET-1 concentration after exercise was significantly higher in horses suffering from cardiac disease than at rest. This suggests that exercise affects the ET-1 levels in horses, especially those with cardiac diseases, making it a pertinent factor in horse cardiac health assessments.

Implications of the Research

  • This study highlights the potential use of ET-1 as a biomarker in detecting horses with changes in left atrial cardiac dimensions, especially when the horses are at rest.
  • Future work can focus on how the ET-1 level changes in relation to the severity of the cardiac diseases, its role in predicting the progression of the disease, or in assessing the effectiveness of the therapeutic interventions.

Cite This Article

APA
Gehlen H, Shety T, El-Zahar H, Hofheinz I. (2018). Measurement of plasma endothelin-1 concentration in healthy horses and horses with cardiac disease during rest and after exercise. J Vet Med Sci, 81(2), 263-268. https://doi.org/10.1292/jvms.18-0325

Publication

The Journal of veterinary medical science
ISSN: 1347-7439
NlmUniqueID: 9105360
Country: Japan
Language: English
Volume: 81
Issue: 2
Pages: 263-268

Researcher Affiliations

Gehlen, Heidrun
  • Equine Clinic, Free University of Berlin, 14163 Berlin, Germany.
Shety, Tarek
  • Animal Medicine Dept., Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
El-Zahar, Heba
  • Animal Medicine Dept., Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Hofheinz, Ina
  • Equine Clinic, Free University of Berlin, 14163 Berlin, Germany.

MeSH Terms

  • Animals
  • Biomarkers / blood
  • Echocardiography / veterinary
  • Electrocardiography / veterinary
  • Endothelin-1 / blood
  • Endothelin-1 / physiology
  • Female
  • Heart Diseases / blood
  • Heart Diseases / physiopathology
  • Heart Diseases / veterinary
  • Horse Diseases / blood
  • Horse Diseases / physiopathology
  • Horses / blood
  • Horses / physiology
  • Male
  • Physical Conditioning, Animal / physiology
  • Rest / physiology

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