FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / J Vet Intern Med / Measurement of serum IgG in foals by radial immunodiffusion ...
Journal of veterinary internal medicine2005; 19(1); 93-96; doi: 10.1892/0891-6640(2005)19<93:mosiif>2.0.co;2

Measurement of serum IgG in foals by radial immunodiffusion and automated turbidimetric immunoassay.

Abstract: Hypogammaglobulinemia as a result of failure of transfer of passive immunity (FTPI) is an important risk factor for infectious disease in neonatal foals. The current gold standard for determining serum immunoglobulin concentrations is radial immunodiffusion (RID). The purpose of this study was to compare immunoglobulin concentrations measured by RID with those determined by an automated turbidimetric immunoassay (TIA), which has a much shorter turnaround time. Immunoglobulin concentrations were measured by both RID and TIA in serum collected from 84 neonatal foals. Sixty-seven foals had results within the linear range for both assays. Sensitivity and specificity of TIA for diagnosis of FTPI with IgG < or = 800 mg/dL were 0.81 (95% CI 0.70-0.88) and 0.86 (95% CI 0.76-0.93) and with IgG < or = 400 mg/dL were 0.63 (95% CI 0.35-0.86) and 0.92 (95% CI 0.87-0.95), respectively. A significant linear relationship was found between IgG concentrations determined by TIA and RID (TIA = 0.9511RID + 8.4354; R2 = .59, P < .0001). The coefficients of variation for between-run and within-run precision for the TIA were 2.5 and 3%, respectively. Storage of samples from 10 foals at -20 degrees C for 10-12 months resulted in a reduction in TIA-measured serum IgG concentration of -17.6% (SD = 3.7%), indicating that long-term storage of samples at -20 degrees C should be avoided. The results of this study indicate that measurement of serum IgG by TIA can be used to evaluate foals for FTPI.
Get Full Text
Publication Date: 2005-02-18 PubMed ID: 15715054DOI: 10.1892/0891-6640(2005)19<93:mosiif>2.0.co;2Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the use of automated turbidimetric immunoassay (TIA) for measuring immunoglobulin concentrations in the serum of neonatal foals, and compares its effectiveness and efficiency with the current standard method, radial immunodiffusion (RID).

Study Purpose & Methods

  • The goal of this study was to validate and compare the effectiveness of two distinct methods for measuring serum immunoglobulin (IgG) concentrations in neonatal foals.
  • RAD is currently considered the ‘gold standard’ for determining serum immunoglobulin concentrations. TDA, however, boasts a significantly shorter turnaround time, making it a potentially more efficient method if it proves to be as reliable.
  • The test subjects were 84 neonatal foals, whose IgG concentrations were measured using both methods.

Key Findings

  • Of the 84 tested, sixty-seven foals had results within valid range for both methods.
  • The sensitivity and specificity values of TIA for diagnosing Failure of Transfer of Passive Immunity (FTPI) were calculated. For concentrations of 800mg/dL and 400mg/dL IgG, they were found to be 0.81 and 0.63 and 0.86 and 0.92, respectively.
  • A significant linear relationship was observed between IgG concentrations as determined by TIA and RID.
  • The variations for between-run and within-run precision for the TIA were only 2.5% and 3%, respectively. This suggests good reliability with the new method.
  • The study also found that storing samples at -20 degrees C for extended periods (10-12 months) resulted in a drop in TIA-measured serum IgG concentration by 17.6%. This indicates that long-term storage of samples at this temperature is not recommended.

Conclusion

  • The findings from this study suggest TIA can be effectively used to evaluate foals for FTPI, which could lead to quicker diagnosis and treatment for the neonatal foals.

Cite This Article

APA
Davis DG, Schaefer DM, Hinchcliff KW, Wellman ML, Willet VE, Fletcher JM. (2005). Measurement of serum IgG in foals by radial immunodiffusion and automated turbidimetric immunoassay. J Vet Intern Med, 19(1), 93-96. https://doi.org/10.1892/0891-6640(2005)19<93:mosiif>2.0.co;2

Publication

Journal of veterinary internal medicine
ISSN: 0891-6640
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 19
Issue: 1
Pages: 93-96

Researcher Affiliations

Davis, Deborah G
  • Department of Veterinary Biosciences, The Ohio State University, Columbus, OH 43210, USA.
Schaefer, Deanna M W
    Hinchcliff, Kenneth W
      Wellman, Maxey L
        Willet, V Ellen
          Fletcher, Jana M

            MeSH Terms

            • Animals
            • Animals, Newborn
            • Horses / immunology
            • Immunodiffusion / methods
            • Immunodiffusion / veterinary
            • Immunoglobulin G / blood
            • Nephelometry and Turbidimetry / methods
            • Nephelometry and Turbidimetry / veterinary
            • Sensitivity and Specificity

            Citations

            This article has been cited 7 times.
            1. Ruiz-Duque B, Bañuls L, Reinoso-Arija R, Carrasco-Hernandez L, Caballero-Eraso C, Dasí F, Lopez-Campos JL. Methodologies for the Determination of Blood Alpha1 Antitrypsin Levels: A Systematic Review.. J Clin Med 2021 Oct 31;10(21).
              doi: 10.3390/jcm10215132pubmed: 34768650google scholar: lookup
            2. Turini L, Bonelli F, Nocera I, Meucci V, Conte G, Sgorbini M. Evaluation of Different Methods to Estimate the Transfer of Immunity in Donkey Foals Fed with Colostrum of Good IgG Quality: A Preliminary Study.. Animals (Basel) 2021 Feb 15;11(2).
              doi: 10.3390/ani11020507pubmed: 33672086google scholar: lookup
            3. Turini L, Bonelli F, Nocera I, Battaglia F, Meucci V, Panzani D, Mele M, Sgorbini M. Evaluation of jennies' colostrum: IgG concentrations and absorption in the donkey foals. A preliminary study.. Heliyon 2020 Aug;6(8):e04598.
              doi: 10.1016/j.heliyon.2020.e04598pubmed: 32775752google scholar: lookup
            4. Parachalil DR, McIntyre J, Byrne HJ. Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances.. Anal Bioanal Chem 2020 Apr;412(9):1993-2007.
              doi: 10.1007/s00216-019-02349-1pubmed: 31900532google scholar: lookup
            5. Ujvari S, Schwarzwald CC, Fouché N, Howard J, Schoster A. Validation of a Point-of-Care Quantitative Equine IgG Turbidimetric Immunoassay and Comparison of IgG Concentrations Measured with Radial Immunodiffusion and a Point-of-Care IgG ELISA.. J Vet Intern Med 2017 Jul;31(4):1170-1177.
              doi: 10.1111/jvim.14770pubmed: 28561898google scholar: lookup
            6. Bakhmachuk A, Gorbatiuk O, Rachkov A, Dons'koi B, Khristosenko R, Ushenin I, Peshkova V, Soldatkin A. Surface Plasmon Resonance Investigations of Bioselective Element Based on the Recombinant Protein A for Immunoglobulin Detection.. Nanoscale Res Lett 2017 Dec;12(1):112.
              doi: 10.1186/s11671-017-1903-5pubmed: 28209032google scholar: lookup
            7. Gilliam LL, Carmichael RC, Holbrook TC, Taylor JM, Ownby CL, McFarlane D, Payton ME. Antibody responses to natural rattlesnake envenomation and a rattlesnake toxoid vaccine in horses.. Clin Vaccine Immunol 2013 May;20(5):732-7.
              doi: 10.1128/CVI.00004-13pubmed: 23515015google scholar: lookup
            Subscribe to our newsletter
            Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.