Measurement of total volume and protein concentration of intrauterine secretion after intrauterine inoculation of bacteria in mares that were either resistant or susceptible to chronic uterine infection.
Abstract: Undiluted uterine secretion was used to determine the concentration of total protein and the accumulated volume of uterine secretion after a bacterial inoculation in mares susceptible and resistant to chronic uterine infection (CUI). The uterus of 6 susceptible and 5 resistant mares was inoculated with 5 x 10(6) Streptococcus zooepidemicus on the third day of estrus. Using a tampon inserted in the uterus, secretions were sampled at 5, 12, 24, and 36 hours after inoculation, followed by intrauterine lavage with phosphate buffered saline solution. The concentration of protein was determined in the undiluted secretion as well as in the uterine washing and the total amount of accumulated uterine secretion was calculated. Protein concentrations in plasma were compared before and after absorption by the tampon. Protein concentration of plasma before and after absorption by the tampon did not differ. Mares susceptible to CUI accumulated significantly (P less than 0.001) more fluid in the uterus than mares resistant to CUI, and uterine washings from the resistant mares were significantly (P less than 0.05) more dilute than those from the susceptible mares. Significant differences in protein concentrations between susceptible and resistant mares were not found. It was concluded from this study that the described method to sample undiluted uterine secretion was practical and reliable for the analysis of protein concentration. Various concentrations of uterine secretions in washings from susceptible and resistant mares emphasizes the importance in using undiluted uterine secretions or dilution markers in washings when intrauterine products are analyzed.
Publication Date: 1992-09-01 PubMed ID: 1416370
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores the differences in uterine secretion characteristics between mares that are resistant or susceptible to chronic uterine infection (CUI). Evidence in the study shows that mares susceptible to CUI produce significantly more fluid in their uteruses than their resistant counterparts.
Research Methodology
- The researchers collected undiluted uterine secretions from a group of 11 mares, 6 of which were susceptible to chronic uterine infection and 5 were resistant.
- Each mare’s uterus was inoculated with a specific bacteria, Streptococcus zooepidemicus, on the third day of estrus.
- Uterine secretions were then sampled at timed intervals of 5, 12, 24, and 36 hours after the inoculation. This was done using a tampon inserted into the uterus.
- After the sampling, the uterus was washed with a phosphate buffered saline solution.
- The concentration of protein in the secretions and the washings was measured, and the total amount of accumulated uterine secretion was calculated.
- The experiment also involved a comparison of protein concentrations in plasma, before and after absorption by the tampon.
Findings
- There was no significant difference recognized in the protein concentration of plasma, before and after absorption by the tampon.
- Susceptible mares showed significantly higher fluid accumulation in the uterus compared to the resistant ones.
- The uterine washings from resistant mares were found to be more dilute than those from susceptible mares.
- There were no significant differences noted in protein concentrations between the two groups of mares.
Conclusions
- The methodology developed for sampling undiluted uterine secretion proved to be practical and reliable for analyzing protein concentration.
- The different concentrations of uterine secretions in washings between susceptible and resistant mares highlighted the importance of using undiluted secretions or dilution markers in washings when analyzing intrauterine products.
Cite This Article
APA
Troedsson MH, Liu IK.
(1992).
Measurement of total volume and protein concentration of intrauterine secretion after intrauterine inoculation of bacteria in mares that were either resistant or susceptible to chronic uterine infection.
Am J Vet Res, 53(9), 1641-1644.
Publication
Researcher Affiliations
- Department of Reproduction, School of Veterinary Medicine, University of California, Davis 95616.
MeSH Terms
- Animals
- Chronic Disease
- Disease Susceptibility
- Endometritis / immunology
- Endometritis / metabolism
- Endometritis / veterinary
- Female
- Horse Diseases / immunology
- Horse Diseases / metabolism
- Horses
- Immunity, Innate
- Proteins / analysis
- Streptococcal Infections / immunology
- Streptococcal Infections / metabolism
- Streptococcal Infections / veterinary
- Tampons, Surgical / veterinary
- Uterus / chemistry
- Uterus / metabolism
Citations
This article has been cited 3 times.- Katila T, Ferreira-Dias G. Evolution of the Concepts of Endometrosis, Post Breeding Endometritis, and Susceptibility of Mares. Animals (Basel) 2022 Mar 19;12(6).
- Adnane M, Meade KG, O'Farrelly C. Cervico-vaginal mucus (CVM) - an accessible source of immunologically informative biomolecules. Vet Res Commun 2018 Dec;42(4):255-263.
- Alavi-Shoushtari SM, Abedizadeh R, Khaki A, Mokarizadeh A, Dorostkar K. A study on the effects of the estrous cycle on uterine fluid and blood serum immunoglobulin G (IgG) content in the cow. Vet Res Forum 2014 Spring;5(2):115-9.
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