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Metabolic and mitogenic activities of insulin-like growth factor-1 in interleukin-1-conditioned equine cartilage.
Abstract: To determine response of interleukin-1alpha (IL-1alpha)-conditioned equine articular cartilage explants to insulin-like growth factor-1 (IGF-1). Sample Population-Cartilage from the trochlea and condyles of the femur of a clinically normal 4-year-old horse. Methods: Effects of IGF-1 (0 to 500 ng/ml) after addition of IL-1alpha were evaluated by assessing matrix responses, using a sulfated glycosaminoglycan (GAG) assay, matrix 35SO4 GAG incorporation, and release of GAG. Mitogenic response was assessed by 3H-thymidine incorporation into DNA and fluorometric assay of total DNA concentration. Results: Human recombinant IL-1alpha (40 ng/ml) increased the amount of labeled GAG released and decreased labeled and total GAG remaining in explants, and IL-1alpha decreased mitogenic response. Addition of IGF-1 counteracted effects seen with IL-1alpha alone. In general, IGF-1 decreased total and labeled GAG released into the medium, compared with IL-1alpha-treated explants (positive-control sample). Values for these variables did not differ significantly from those for negative-control explants. A significant increase in total and newly synthesized GAG in the explants at termination of the experiment was observed with 500 ng of IGF-1/ml. Labeled GAG remaining in explants was greater with treatment at 50 ng of IGF-1/ml, compared with treatment with IL-1alpha alone. Concentrations of 200 ng of IGF-1/ml abolished actions of IL-1alpha and restored DNA synthesis to values similar to those of negative-control explants. Conclusions: IGF-1 at 500 ng/ml was best at overcoming detrimental effects associated with IL-1alpha in in vitro explants. These beneficial effects may be useful in horses with osteoarthritis.
Publication Date: 2000-04-20 PubMed ID: 10772110DOI: 10.2460/ajvr.2000.61.436Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article investigates the effects of insulin-like growth factor-1 (IGF-1) on interleukin-1alpha (IL-1alpha)-conditioned equine articular cartilage. The researchers found that IGF-1 can neutralize the adverse effects of IL-1alpha, and may be useful in treating horses with osteoarthritis.
Methods and Sample Population
- The study was conducted using cartilage from the trochlea and condyles of the femur of a clinically normal 4-year-old horse.
- The cartilage explants were conditioned with interleukin-1alpha (IL-1alpha) and then treated with varying concentrations of insulin-like growth factor-1 (IGF-1).
- The researchers then evaluated the matrix responses, looking specifically at the effects on sulfated glycosaminoglycan (GAG).
- The mitogenic response was assessed by measuring the incorporation of 3H-thymidine into DNA and using a fluorometric assay of total DNA concentration.
Results
- The study found that IL-1alpha increased the amount of labeled GAG released and decreased labeled and total GAG remaining in explants. IL-1alpha also decreased mitogenic response.
- The addition of IGF-1 effectively counteracted the effects observed with IL-1alpha alone.
- Compared with IL-1alpha-treated explants, IGF-1 generally decreased total and labeled GAG release into the medium.
- Applying 500 ng/ml of IGF-1 led to a significant increase in total and newly synthesized GAG in the explants at the end of the experiment. This concentration of IGF-1 was the most effective at overcoming the adverse effects associated with IL-1alpha.
- At 200 ng/ml, IGF-1 negated the actions of IL-1alpha and restored DNA synthesis to levels similar to the negative-control explants.
Conclusions
- The results of the study suggest that IGF-1 has the potential to neutralize the detrimental effects of IL-1alpha on equine articular cartilage.
- This implies that IGF-1 treatment may be beneficial for horses suffering from osteoarthritis.
Cite This Article
APA
Frisbie DD, Sandler EA, Trotter GW, McIlwraith CW.
(2000).
Metabolic and mitogenic activities of insulin-like growth factor-1 in interleukin-1-conditioned equine cartilage.
Am J Vet Res, 61(4), 436-441.
https://doi.org/10.2460/ajvr.2000.61.436 Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
MeSH Terms
- Animals
- Cartilage / drug effects
- Cartilage / metabolism
- Culture Media
- Culture Techniques
- DNA Replication / drug effects
- Glycosaminoglycans / metabolism
- Horses / metabolism
- Humans
- Insulin-Like Growth Factor I / metabolism
- Interleukin-1 / pharmacology
- Mitosis / drug effects
- Recombinant Proteins / pharmacology
Citations
This article has been cited 1 times.- Tung JT, Fenton JI, Arnold C, Alexander L, Yuzbasiyan-Gurkan V, Venta PJ, Peters TL, Orth MW, Richardson DW, Caron JP. Recombinant equine interleukin-1beta induces putative mediators of articular cartilage degradation in equine chondrocytes. Can J Vet Res 2002 Jan;66(1):19-25.
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