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Acta veterinaria Scandinavica2009; 51(1); 10; doi: 10.1186/1751-0147-51-10

Metabolism during anaesthesia and recovery in colic and healthy horses: a microdialysis study.

Abstract: Muscle metabolism in horses has been studied mainly by analysis of substances in blood or plasma and muscle biopsy specimens. By using microdialysis, real-time monitoring of the metabolic events in local tissue with a minimum of trauma is possible. There is limited information about muscle metabolism in the early recovery period after anaesthesia in horses and especially in the colic horse. The aims were to evaluate the microdialysis technique as a complement to plasma analysis and to study the concentration changes in lactate, pyruvate, glucose, glycerol, and urea during anaesthesia and in the recovery period in colic horses undergoing abdominal surgery and in healthy horses not subjected to surgery. Methods: Ten healthy university-owned horses given anaesthesia alone and ten client-owned colic horses subjected to emergency abdominal surgery were anaesthetised for a mean (range) of 230 min (193-273) and 208 min (145-300) respectively. Venous blood samples were taken before anaesthesia. Venous blood sampling and microdialysis in the gluteal muscle were performed during anaesthesia and until 24 h after anaesthesia. Temporal changes and differences between groups were analysed with an ANOVA for repeated measures followed by Tukey Post Hoc test or Planned Comparisons. Results: Lactate, glucose and urea, in both dialysate and plasma, were higher in the colic horses than in the healthy horses for several hours after recovery to standing. In the colic horses, lactate, glucose, and urea in dialysate, and lactate in plasma increased during the attempts to stand. The lactate-to-pyruvate ratio was initially high in sampled colic horses but decreased over time. In the colic horses, dialysate glycerol concentrations varied considerably whereas in the healthy horses, dialysate glycerol was elevated during anaesthesia but decreased after standing. In both groups, lactate concentration was higher in dialysate than in plasma. The correspondence between dialysate and plasma concentrations of glucose, urea and glycerol varied. Conclusions: Microdialysis proved to be suitable in the clinical setting for monitoring of the metabolic events during anaesthesia and recovery. It was possible with this technique to show greater muscle metabolic alterations in the colic horses compared to the healthy horses in response to regaining the standing position.
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Publication Date: 2009-03-10 PubMed ID: 19284560PubMed Central: PMC2660341DOI: 10.1186/1751-0147-51-10Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the changes in muscle metabolism in horses, specifically colic horses undergoing abdominal surgery and healthy ones not subjected to surgery, during anesthetic and recovery periods. It demonstrates how microdialysis can effectively monitor these metabolic changes with minimal trauma.

Objective of the Study

  • The researchers aim to use the microdialysis technique as a supplement to plasma analysis to study the shifts in lactate, pyruvate, glucose, glycerol, and urea concentrations during anaesthesia and post-anaesthesia recovery periods in colic horses and healthy horses.

Methods

  • The study included ten healthy horses given anaesthesia only and ten colic horses subjected to emergency abdominal surgery. The average duration of anaesthesia for both groups was around 219 minutes.
  • Researchers took venous blood samples before anaesthesia and continued venous blood sampling and microdialysis in the gluteal muscle during anaesthesia and for up to 24 hours post-anaesthesia.
  • They then analyzed the temporal changes and group differences using ANOVA for repeated measures, followed by the Tukey Post Hoc test or Planned Comparisons.

Results

  • For several hours post-recovery, lactate, glucose, and urea, in both dialysate and plasma, were higher in colic horses than in healthy horses.
  • Lactate, glucose, and urea in dialysate and lactate in plasma significantly increased in colic horses during attempts to stand.
  • The lactate-to-pyruvate ratio was initially high in the colic horses but decreased over time, indicating changes in muscle metabolism.
  • Dialysate glycerol concentrations were considerably varied in colic horses, whilst in healthy horses, there was an elevation during anaesthesia which decreased after standing.
  • Lactate concentration was consistently higher in dialysate than in plasma for both groups. The correspondence between dialysate and plasma levels of glucose, urea, and glycerol varied.

Conclusion

  • Microdialysis proved to be a viable clinical technique to monitor metabolic events during anaesthesia and recovery.
  • With this method, researchers could document more pronounced muscle metabolic alterations in colic horses than in healthy ones upon regaining the standing position post-anaesthesia.

Cite This Article

APA
Edner AH, Essén-Gustavsson B, Nyman GC. (2009). Metabolism during anaesthesia and recovery in colic and healthy horses: a microdialysis study. Acta Vet Scand, 51(1), 10. https://doi.org/10.1186/1751-0147-51-10

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 51
Issue: 1
Pages: 10

Researcher Affiliations

Edner, Anna H
  • Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden. anna.edner@kv.slu.se
Essén-Gustavsson, Birgitta
    Nyman, Görel C

      MeSH Terms

      • Anesthesia / veterinary
      • Animals
      • Colic / metabolism
      • Colic / surgery
      • Colic / veterinary
      • Female
      • Horse Diseases / blood
      • Horse Diseases / metabolism
      • Horse Diseases / surgery
      • Horses
      • Male
      • Microdialysis / methods
      • Microdialysis / veterinary

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      Citations

      This article has been cited 1 times.
      1. Gozalo-Marcilla M, Ringer SK. Recovery after General Anaesthesia in Adult Horses: A Structured Summary of the Literature. Animals (Basel) 2021 Jun 14;11(6).
        doi: 10.3390/ani11061777pubmed: 34198637google scholar: lookup
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