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Home / Equine Research Bank / Acta Vet Scand / Metabolism during anaesthesia and recovery in colic and heal...
Acta veterinaria Scandinavica2009; 51(1); 10; doi: 10.1186/1751-0147-51-10

Metabolism during anaesthesia and recovery in colic and healthy horses: a microdialysis study.

Abstract: Muscle metabolism in horses has been studied mainly by analysis of substances in blood or plasma and muscle biopsy specimens. By using microdialysis, real-time monitoring of the metabolic events in local tissue with a minimum of trauma is possible. There is limited information about muscle metabolism in the early recovery period after anaesthesia in horses and especially in the colic horse. The aims were to evaluate the microdialysis technique as a complement to plasma analysis and to study the concentration changes in lactate, pyruvate, glucose, glycerol, and urea during anaesthesia and in the recovery period in colic horses undergoing abdominal surgery and in healthy horses not subjected to surgery. Methods: Ten healthy university-owned horses given anaesthesia alone and ten client-owned colic horses subjected to emergency abdominal surgery were anaesthetised for a mean (range) of 230 min (193-273) and 208 min (145-300) respectively. Venous blood samples were taken before anaesthesia. Venous blood sampling and microdialysis in the gluteal muscle were performed during anaesthesia and until 24 h after anaesthesia. Temporal changes and differences between groups were analysed with an ANOVA for repeated measures followed by Tukey Post Hoc test or Planned Comparisons. Results: Lactate, glucose and urea, in both dialysate and plasma, were higher in the colic horses than in the healthy horses for several hours after recovery to standing. In the colic horses, lactate, glucose, and urea in dialysate, and lactate in plasma increased during the attempts to stand. The lactate-to-pyruvate ratio was initially high in sampled colic horses but decreased over time. In the colic horses, dialysate glycerol concentrations varied considerably whereas in the healthy horses, dialysate glycerol was elevated during anaesthesia but decreased after standing. In both groups, lactate concentration was higher in dialysate than in plasma. The correspondence between dialysate and plasma concentrations of glucose, urea and glycerol varied. Conclusions: Microdialysis proved to be suitable in the clinical setting for monitoring of the metabolic events during anaesthesia and recovery. It was possible with this technique to show greater muscle metabolic alterations in the colic horses compared to the healthy horses in response to regaining the standing position.
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Publication Date: 2009-03-10 PubMed ID: 19284560PubMed Central: PMC2660341DOI: 10.1186/1751-0147-51-10Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the changes in muscle metabolism in horses, specifically colic horses undergoing abdominal surgery and healthy ones not subjected to surgery, during anesthetic and recovery periods. It demonstrates how microdialysis can effectively monitor these metabolic changes with minimal trauma.

Objective of the Study

  • The researchers aim to use the microdialysis technique as a supplement to plasma analysis to study the shifts in lactate, pyruvate, glucose, glycerol, and urea concentrations during anaesthesia and post-anaesthesia recovery periods in colic horses and healthy horses.

Methods

  • The study included ten healthy horses given anaesthesia only and ten colic horses subjected to emergency abdominal surgery. The average duration of anaesthesia for both groups was around 219 minutes.
  • Researchers took venous blood samples before anaesthesia and continued venous blood sampling and microdialysis in the gluteal muscle during anaesthesia and for up to 24 hours post-anaesthesia.
  • They then analyzed the temporal changes and group differences using ANOVA for repeated measures, followed by the Tukey Post Hoc test or Planned Comparisons.

Results

  • For several hours post-recovery, lactate, glucose, and urea, in both dialysate and plasma, were higher in colic horses than in healthy horses.
  • Lactate, glucose, and urea in dialysate and lactate in plasma significantly increased in colic horses during attempts to stand.
  • The lactate-to-pyruvate ratio was initially high in the colic horses but decreased over time, indicating changes in muscle metabolism.
  • Dialysate glycerol concentrations were considerably varied in colic horses, whilst in healthy horses, there was an elevation during anaesthesia which decreased after standing.
  • Lactate concentration was consistently higher in dialysate than in plasma for both groups. The correspondence between dialysate and plasma levels of glucose, urea, and glycerol varied.

Conclusion

  • Microdialysis proved to be a viable clinical technique to monitor metabolic events during anaesthesia and recovery.
  • With this method, researchers could document more pronounced muscle metabolic alterations in colic horses than in healthy ones upon regaining the standing position post-anaesthesia.

Cite This Article

APA
Edner AH, Essén-Gustavsson B, Nyman GC. (2009). Metabolism during anaesthesia and recovery in colic and healthy horses: a microdialysis study. Acta Vet Scand, 51(1), 10. https://doi.org/10.1186/1751-0147-51-10

Publication

Acta veterinaria Scandinavica
ISSN: 1751-0147
NlmUniqueID: 0370400
Country: England
Language: English
Volume: 51
Issue: 1
Pages: 10

Researcher Affiliations

Edner, Anna H
  • Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Uppsala, Sweden. anna.edner@kv.slu.se
Essén-Gustavsson, Birgitta
    Nyman, Görel C

      MeSH Terms

      • Anesthesia / veterinary
      • Animals
      • Colic / metabolism
      • Colic / surgery
      • Colic / veterinary
      • Female
      • Horse Diseases / blood
      • Horse Diseases / metabolism
      • Horse Diseases / surgery
      • Horses
      • Male
      • Microdialysis / methods
      • Microdialysis / veterinary

      References

      This article includes 56 references
      1. Ungerstedt U, Hallström A. In vivo microdialysis--a new approach to the analysis of neurotransmitters in the brain.. Life Sci 1987 Aug 17;41(7):861-4.
        doi: 10.1016/0024-3205(87)90181-0pubmed: 2886879google scholar: lookup
      2. Ingvast-Larsson C, Appelgren LE, Nyman G. Distribution studies of theophylline: microdialysis in rat and horse and whole body autoradiography in rat.. J Vet Pharmacol Ther 1992 Dec;15(4):386-94.
        doi: 10.1111/j.1365-2885.1992.tb01030.xpubmed: 1487837google scholar: lookup
      3. Hagström-Toft E, Enoksson S, Moberg E, Bolinder J, Arner P. Absolute concentrations of glycerol and lactate in human skeletal muscle, adipose tissue, and blood.. Am J Physiol 1997 Sep;273(3 Pt 1):E584-92.
        pubmed: 9316449doi: 10.1152/ajpendo.1997.273.3.e584google scholar: lookup
      4. Groth L, Serup J. Cutaneous microdialysis in man: effects of needle insertion trauma and anaesthesia on skin perfusion, erythema and skin thickness.. Acta Derm Venereol 1998 Jan;78(1):5-9.
        doi: 10.1080/00015559850135733pubmed: 9498017google scholar: lookup
      5. Ettinger SN, Poellmann CC, Wisniewski NA, Gaskin AA, Shoemaker JS, Poulson JM, Dewhirst MW, Klitzman B. Urea as a recovery marker for quantitative assessment of tumor interstitial solutes with microdialysis.. Cancer Res 2001 Nov 1;61(21):7964-70.
        pubmed: 11691820
      6. Ungerstedt J, Nowak G, Ericzon BG, Ungerstedt U. Intraperitoneal microdialysis (IPM): a new technique for monitoring intestinal ischemia studied in a porcine model.. Shock 2003 Jul;20(1):91-6.
        doi: 10.1097/01.shk.0000070904.21762.36pubmed: 12813375google scholar: lookup
      7. Rooyackers O, Thorell A, Nygren J, Ljungqvist O. Microdialysis methods for measuring human metabolism.. Curr Opin Clin Nutr Metab Care 2004 Sep;7(5):515-21.
        doi: 10.1097/00075197-200409000-00003pubmed: 15295271google scholar: lookup
      8. Rosdahl H, Hamrin K, Ungerstedt U, Henriksson J. Metabolite levels in human skeletal muscle and adipose tissue studied with microdialysis at low perfusion flow.. Am J Physiol 1998 May;274(5):E936-45.
        pubmed: 9612253doi: 10.1152/ajpendo.1998.274.5.e936google scholar: lookup
      9. Ungerstedt U. Microdialysis--principles and applications for studies in animals and man.. J Intern Med 1991 Oct;230(4):365-73.
        pubmed: 1919432doi: 10.1111/j.1365-2796.1991.tb00459.xgoogle scholar: lookup
      10. Jansson K, Ungerstedt J, Jonsson T, Redler B, Andersson M, Ungerstedt U, Norgren L. Human intraperitoneal microdialysis: increased lactate/pyruvate ratio suggests early visceral ischaemia. A pilot study.. Scand J Gastroenterol 2003 Sep;38(9):1007-11.
        doi: 10.1080/00365520310004065pubmed: 14531543google scholar: lookup
      11. Luchette FA, Jenkins WA, Friend LA, Su C, Fischer JE, James JH. Hypoxia is not the sole cause of lactate production during shock.. J Trauma 2002 Mar;52(3):415-9.
        doi: 10.1097/00005373-200203000-00001pubmed: 11901313google scholar: lookup
      12. Edner A, Nyman G, Essén-Gustavsson B. The relationship of muscle perfusion and metabolism with cardiovascular variables before and after detomidine injection during propofol-ketamine anaesthesia in horses.. Vet Anaesth Analg 2002 Oct;29(4):182-199.
        doi: 10.1046/j.1467-2995.2002.00101.xpubmed: 28404362google scholar: lookup
      13. Edner A, Essén-Gustavsson B, Nyman G. Muscle metabolic changes associated with long-term inhalation anaesthesia in the horse analysed by muscle biopsy and microdialysis techniques.. J Vet Med A Physiol Pathol Clin Med 2005 Mar;52(2):99-107.
        pubmed: 15737180doi: 10.1111/j.1439-0442.2004.00685.xgoogle scholar: lookup
      14. Manohar M, Gustafson R, Goetz TE, Nganwa D. Systemic distribution of blood flow in ponies during 1.45%, 1.96%, and 2.39% end-tidal isoflurane-O2 anesthesia.. Am J Vet Res 1987 Oct;48(10):1504-10.
        pubmed: 3674561
      15. Goetz TE, Manohar M, Nganwa D, Gustafson R. A study of the effect of isoflurane anaesthesia on equine skeletal muscle perfusion.. Equine Vet J Suppl 1989 Jun;(7):133-7.
        pubmed: 9118096doi: 10.1111/j.2042-3306.1989.tb05673.xgoogle scholar: lookup
      16. Serteyn D, Mottart E, Michaux C, Micheels J, Philippart C, Lavergne L, Guillon C, Lamy M. Laser Doppler flowmetry: muscular microcirculation in anaesthetized horses.. Equine Vet J 1986 Sep;18(5):391-5.
        pubmed: 2945716doi: 10.1111/j.2042-3306.1986.tb03663.xgoogle scholar: lookup
      17. Moore JN, Owen RR, Lumsden JH. Clinical evaluation of blood lactate levels in equine colic.. Equine Vet J 1976 Apr;8(2):49-54.
        pubmed: 4300doi: 10.1111/j.2042-3306.1976.tb03289.xgoogle scholar: lookup
      18. Parry BW, Anderson GA, Gay CC. Prognosis in equine colic: a study of individual variables used in case assessment.. Equine Vet J 1983 Oct;15(4):337-44.
        pubmed: 6641680doi: 10.1111/j.2042-3306.1983.tb01818.xgoogle scholar: lookup
      19. Ihler CF, Venger JL, Skjerve E. Evaluation of clinical and laboratory variables as prognostic indicators in hospitalised gastrointestinal colic horses.. Acta Vet Scand 2004;45(1-2):109-18.
        doi: 10.1186/1751-0147-45-109pmc: PMC1820986pubmed: 15535091google scholar: lookup
      20. Edner AH, Nyman GC, Essén-Gustavsson B. Metabolism before, during and after anaesthesia in colic and healthy horses.. Acta Vet Scand 2007 Nov 15;49(1):34.
        pmc: PMC2206032pubmed: 18001483doi: 10.1186/1751-0147-49-34google scholar: lookup
      21. Rosdahl H, Ungerstedt U, Henriksson J. Microdialysis in human skeletal muscle and adipose tissue at low flow rates is possible if dextran-70 is added to prevent loss of perfusion fluid.. Acta Physiol Scand 1997 Mar;159(3):261-2.
        doi: 10.1046/j.1365-201X.1997.123358000.xpubmed: 9079158google scholar: lookup
      22. Lowry OH, Passonneau JV. A flexible system of enzymatic analysis. New York: Academic Press; 1973. pp. 1–291.
      23. Delgado JM, DeFeudis FV, Roth RH, Ryugo DK, Mitruka BM. Dialytrode for long term intracerebral perfusion in awake monkeys.. Arch Int Pharmacodyn Ther 1972;198(1):9-21.
        pubmed: 4626478
      24. Abrahamsson P, Johansson G, Aberg AM, Haney M, Winsö O. Optimised sample handling in association with use of the CMA 600 analyser.. J Pharm Biomed Anal 2008 Nov 4;48(3):940-5.
        doi: 10.1016/j.jpba.2008.08.010pubmed: 18809273google scholar: lookup
      25. Parry BW, Anderson GA, Gay CC. Prognosis in equine colic: a comparative study of variables used to assess individual cases.. Equine Vet J 1983 Jul;15(3):211-5.
        pubmed: 6884310doi: 10.1111/j.2042-3306.1983.tb01768.xgoogle scholar: lookup
      26. Gladden LB. Lactate metabolism: a new paradigm for the third millennium.. J Physiol 2004 Jul 1;558(Pt 1):5-30.
        doi: 10.1113/jphysiol.2003.058701pmc: PMC1664920pubmed: 15131240google scholar: lookup
      27. James JH, Luchette FA, McCarter FD, Fischer JE. Lactate is an unreliable indicator of tissue hypoxia in injury or sepsis.. Lancet 1999 Aug 7;354(9177):505-8.
        doi: 10.1016/S0140-6736(98)91132-1pubmed: 10465191google scholar: lookup
      28. Gore DC, Jahoor F, Hibbert JM, DeMaria EJ. Lactic acidosis during sepsis is related to increased pyruvate production, not deficits in tissue oxygen availability.. Ann Surg 1996 Jul;224(1):97-102.
        doi: 10.1097/00000658-199607000-00015pmc: PMC1235253pubmed: 8678625google scholar: lookup
      29. Wolfe RR, Martini WZ. Changes in intermediary metabolism in severe surgical illness.. World J Surg 2000 Jun;24(6):639-47.
        doi: 10.1007/s002689910105pubmed: 10773115google scholar: lookup
      30. HUCKABEE WE. Relationships of pyruvate and lactate during anaerobic metabolism. III. Effect of breathing low-oxygen gases.. J Clin Invest 1958 Feb;37(2):264-71.
        doi: 10.1172/JCI103605pmc: PMC293083pubmed: 13513757google scholar: lookup
      31. Larsson J, Hultman E. The effect of long-term arterial occlusion on energy metabolism of the human quadriceps muscle.. Scand J Clin Lab Invest 1979 May;39(3):257-64.
        doi: 10.3109/00365517909106103pubmed: 523975google scholar: lookup
      32. Harris K, Walker PM, Mickle DA, Harding R, Gatley R, Wilson GJ, Kuzon B, McKee N, Romaschin AD. Metabolic response of skeletal muscle to ischemia.. Am J Physiol 1986 Feb;250(2 Pt 2):H213-20.
        pubmed: 3946620doi: 10.1152/ajpheart.1986.250.2.h213google scholar: lookup
      33. Müller M, Schmid R, Nieszpaur-Los M, Fassolt A, Lönnroth P, Fasching P, Eichler HG. Key metabolite kinetics in human skeletal muscle during ischaemia and reperfusion: measurement by microdialysis.. Eur J Clin Invest 1995 Aug;25(8):601-7.
        doi: 10.1111/j.1365-2362.1995.tb01752.xpubmed: 7589017google scholar: lookup
      34. Gladden LB. Muscle as a consumer of lactate.. Med Sci Sports Exerc 2000 Apr;32(4):764-71.
        doi: 10.1097/00005768-200004000-00008pubmed: 10776895google scholar: lookup
      35. Ungerstedt J, Goiny M, Ungerstedt U. Acute compartment syndrome in a pig model monitored with microdialysis. CMA/Microdialysis 3rd Scandinavian Microdialysis User Symposium; Bålsta, Sweden; 2002.
      36. Setälä LP, Korvenoja EM, Härmä MA, Alhava EM, Uusaro AV, Tenhunen JJ. Glucose, lactate, and pyruvate response in an experimental model of microvascular flap ischemia and reperfusion: a microdialysis study.. Microsurgery 2004;24(3):223-31.
        doi: 10.1002/micr.20045pubmed: 15160382google scholar: lookup
      37. Fuchi T, Rosdahl H, Hickner RC, Ungerstedt U, Henricksson J. Microdialysis of rat skeletal muscle and adipose tissue: dynamics of the interstitial glucose pool.. Acta Physiol Scand 1994 Jun;151(2):249-60.
        doi: 10.1111/j.1748-1716.1994.tb09744.xpubmed: 7942061google scholar: lookup
      38. Rosdahl H, Ungerstedt U, Jorfeldt L, Henriksson J. Interstitial glucose and lactate balance in human skeletal muscle and adipose tissue studied by microdialysis.. J Physiol 1993 Nov;471:637-57.
        pmc: PMC1143981pubmed: 8120827doi: 10.1113/jphysiol.1993.sp019920google scholar: lookup
      39. Rosdahl H. Microdialysis sampling from skeletal muscle and adipose tissue with special reference to the effects of insulin on tissue blood flow and glucose metabolism. Doctoral thesis. Karolinska Institutet, the Department of Physiology and Pharmacology; 1998.
      40. Hickner RC, Rosdahl H, Borg I, Ungerstedt U, Jorfeldt L, Henriksson J. The ethanol technique of monitoring local blood flow changes in rat skeletal muscle: implications for microdialysis.. Acta Physiol Scand 1992 Sep;146(1):87-97.
        doi: 10.1111/j.1748-1716.1992.tb09396.xpubmed: 1442130google scholar: lookup
      41. Newsholme EA, Leech AR. Biochemistry for the medical sciences. London, UK: John Wiley & Sons Ltd.; 1983.
      42. Essén B, Kaijser L. Regulation of glycolysis in intermittent exercise in man.. J Physiol 1978 Aug;281:499-511.
        pmc: PMC1282711pubmed: 702403doi: 10.1113/jphysiol.1978.sp012436google scholar: lookup
      43. Gottlieb M. Muscle glycogen depletion patterns during draught work in Standardbred horses.. Equine Vet J 1989 Mar;21(2):110-5.
        pubmed: 2707226doi: 10.1111/j.2042-3306.1989.tb02109.xgoogle scholar: lookup
      44. Kerckhoffs DA, Arner P, Bolinder J. Lipolysis and lactate production in human skeletal muscle and adipose tissue following glucose ingestion.. Clin Sci (Lond) 1998 Jan;94(1):71-7.
        pubmed: 9505869doi: 10.1042/cs0940071google scholar: lookup
      45. Del Canale S, Fiaccadori E, Ronda N, Söderlund K, Antonucci C, Guariglia A. Muscle energy metabolism in uremia.. Metabolism 1986 Nov;35(11):981-3.
        doi: 10.1016/0026-0495(86)90032-6pubmed: 3773727google scholar: lookup
      46. Lager I. The insulin-antagonistic effect of the counterregulatory hormones.. J Intern Med Suppl 1991;735:41-7.
        pubmed: 2043222
      47. Parry BW. Use of clinical pathology in evaluation of horses with colic.. Vet Clin North Am Equine Pract 1987 Dec;3(3):529-42.
        pubmed: 3322525doi: 10.1016/s0749-0739(17)30663-6google scholar: lookup
      48. Svendsen CK, Hjortkjaer RK, Hesselholt M. Colic in the horse. A clinical and clinical chemical study of 42 cases.. Nord Vet Med 1979 Oct;31(10):Suppl 1-32.
        pubmed: 523309
      49. Lundberg G, Olofsson P, Ungerstedt U, Jansson E, Sundberg CJ. Lactate concentrations in human skeletal muscle biopsy, microdialysate and venous blood during dynamic exercise under blood flow restriction.. Pflugers Arch 2002 Jan;443(3):458-65.
        doi: 10.1007/s004240100706pubmed: 11810217google scholar: lookup
      50. Henriksson J. Microdialysis of skeletal muscle at rest.. Proc Nutr Soc 1999 Nov;58(4):919-23.
        doi: 10.1017/S0029665199001226pubmed: 10817159google scholar: lookup
      51. Hagström-Toft E, Enoksson S, Moberg E, Bolinder J, Arner P. beta-Adrenergic regulation of lipolysis and blood flow in human skeletal muscle in vivo.. Am J Physiol 1998 Dec;275(6):E909-16.
        pubmed: 9843731doi: 10.1152/ajpendo.1998.275.6.e909google scholar: lookup
      52. Ostman B, Michaelsson K, Rahme H, Hillered L. Tourniquet-induced ischemia and reperfusion in human skeletal muscle.. Clin Orthop Relat Res 2004 Jan;(418):260-5.
        pubmed: 15043128doi: 10.1097/00003086-200401000-00045google scholar: lookup
      53. Lindsay WA, McDonell W, Bignell W. Equine postanesthetic forelimb lameness: intracompartmental muscle pressure changes and biochemical patterns.. Am J Vet Res 1980 Dec;41(12):1919-24.
        pubmed: 7212425
      54. Lindsay WA, McDonell W, Bignell W. Equine postanesthetic forelimb lameness: intracompartmental muscle pressure changes and biochemical patterns.. Am J Vet Res 1980 Dec;41(12):1919-24.
        pubmed: 7212425
      55. Serteyn D, Mottart E, Deby C, Deby-Dupont G, Pincemail J, Philipart C, Lamy M. Equine postanaesthetic myositis: a possible role for free radical generation and membrane lipoperoxidation.. Res Vet Sci 1990 Jan;48(1):42-6.
        pubmed: 2300714
      56. Serteyn D, Pincemail J, Mottart E, Caudron I, Deby C, Deby-Dupont G, Philippart C, Lamy M. [Direct approach for demonstrating free radical phenomena during equine postanesthetic myopathy: preliminary study].. Can J Vet Res 1994 Oct;58(4):309-12.
        pmc: PMC1263720pubmed: 7889465

      Citations

      This article has been cited 1 times.
      1. Gozalo-Marcilla M, Ringer SK. Recovery after General Anaesthesia in Adult Horses: A Structured Summary of the Literature. Animals (Basel) 2021 Jun 14;11(6).
        doi: 10.3390/ani11061777pubmed: 34198637google scholar: lookup
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