Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age.
Abstract: Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanced age of horses is desirable. That is why the identification of aging-related changes in the horse genome is interesting in this respect. In the recent years, the research on aging includes studies of age-related epigenetic effects observed on the DNA methylation level. We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. Genes colocated with age-related differentially methylated regions (age DMRs) are the members of pathways involved in cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression. A positive correlation was found between methylation states and gene expression in particular loci from our data set. Some of described age DMR-linked genes were also reported elsewhere. Obtained results contribute to the knowledge about the molecular basis of aging of equine blood cells.
Publication Date: 2019-06-02 PubMed ID: 31281827PubMed Central: PMC6589255DOI: 10.1155/2019/2839614Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article focuses on examining changes in the horse genome related to aging, particularly by studying the DNA methylation level. Through this study, a number of age-related differential methylation regions (DMRs) in the genomes of blood leukocytes derived from both juvenile and aged horses of the native Hucul breed have been identified.
Objective and Methodology
- This study aimed to identify the aging-related changes in the horse genome with a particular focus on DNA methylation level.
- Reduced representation bisulfite sequencing (RRBS) was used as the primary method for the demonstration of age-related DNA methylation regions (DMRs).
- The species used in the research were juvenile and aged horses from the native Hucul breed.
Findings
- The researchers found a range of age DMRs in the genomes of blood leukocytes derived from the horses.
- The genes associated with the age-related DMRs are involved in a variety of physiological processes such as cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression.
- The researchers identified a positive correlation between methylation states and gene expression in some specific loci from their data set.
Significance
- The findings of this research contribute to the knowledge about the molecular basis of aging of equine blood cells.
- By understanding the age-related changes to the horse genome, there is potential to discover new solutions in the prevention and treatment of pathological conditions associated with advanced age in horses.
- Some of the age DMR-linked genes discovered in this research have also been reported in other studies, further reinforcing their relevance.
Cite This Article
APA
Ząbek T, Semik-Gurgul E, Szmatoła T, Gurgul A, Fornal A, Bugno-Poniewierska M.
(2019).
Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age.
Int J Genomics, 2019, 2839614.
https://doi.org/10.1155/2019/2839614 Publication
Researcher Affiliations
- National Research Institute of Animal Production, Balice 32-083, Poland.
- National Research Institute of Animal Production, Balice 32-083, Poland.
- National Research Institute of Animal Production, Balice 32-083, Poland.
- University Centre of Veterinary Medicine, University of Agriculture in Kraków, Al. Mickiewicza 24/28, 30-059 Kraków, Poland.
- National Research Institute of Animal Production, Balice 32-083, Poland.
- National Research Institute of Animal Production, Balice 32-083, Poland.
- Institute of Veterinary Sciences, University of Agriculture in Krakow, 31-120, Poland.
References
This article includes 29 references
- Horohov DW, Kydd JH, Hannant D. The effect of aging on T cell responses in the horse.. Dev Comp Immunol 2002 Jan;26(1):121-8.
- Paradis MR. Demographics of health and disease in the geriatric horse.. Vet Clin North Am Equine Pract 2002 Dec;18(3):391-401.
- Deaton AM, Bird A. CpG islands and the regulation of transcription.. Genes Dev 2011 May 15;25(10):1010-22.
- Sun L, Gong Z, Oberst EJ, Betancourt A, Adams AA, Horohov DW. The promoter region of interferon-gamma is hypermethylated in neonatal foals and its demethylation is associated with increased gene expression.. Dev Comp Immunol 2013 Mar;39(3):273-8.
- Bell JT, Tsai PC, Yang TP, Pidsley R, Nisbet J, Glass D, Mangino M, Zhai G, Zhang F, Valdes A, Shin SY, Dempster EL, Murray RM, Grundberg E, Hedman AK, Nica A, Small KS, Dermitzakis ET, McCarthy MI, Mill J, Spector TD, Deloukas P. Epigenome-wide scans identify differentially methylated regions for age and age-related phenotypes in a healthy ageing population.. PLoS Genet 2012;8(4):e1002629.
- Bell CG, Xia Y, Yuan W, Gao F, Ward K, Roos L, Mangino M, Hysi PG, Bell J, Wang J, Spector TD. Novel regional age-associated DNA methylation changes within human common disease-associated loci.. Genome Biol 2016 Sep 23;17(1):193.
- Horvath S, Gurven M, Levine ME, Trumble BC, Kaplan H, Allayee H, Ritz BR, Chen B, Lu AT, Rickabaugh TM, Jamieson BD, Sun D, Li S, Chen W, Quintana-Murci L, Fagny M, Kobor MS, Tsao PS, Reiner AP, Edlefsen KL, Absher D, Assimes TL. An epigenetic clock analysis of race/ethnicity, sex, and coronary heart disease.. Genome Biol 2016 Aug 11;17(1):171.
- Purzyc H. A general characteristic of Hucul horses. Acta Scientiarum Polonorum-Medicina Veterinaria 2007;6(4):25–31.
- Akalin A, Kormaksson M, Li S, Garrett-Bakelman FE, Figueroa ME, Melnick A, Mason CE. methylKit: a comprehensive R package for the analysis of genome-wide DNA methylation profiles.. Genome Biol 2012 Oct 3;13(10):R87.
- Rohde C, Zhang Y, Reinhardt R, Jeltsch A. BISMA--fast and accurate bisulfite sequencing data analysis of individual clones from unique and repetitive sequences.. BMC Bioinformatics 2010 May 6;11:230.
- Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden TL. Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction.. BMC Bioinformatics 2012 Jun 18;13:134.
- Cappelli K, Felicetti M, Capomaccio S, Spinsanti G, Silvestrelli M, Supplizi AV. Exercise induced stress in horses: selection of the most stable reference genes for quantitative RT-PCR normalization.. BMC Mol Biol 2008 May 19;9:49.
- Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR.. Nucleic Acids Res 2001 May 1;29(9):e45.
- Chu YP, Chang CH, Shiu JH, Chang YT, Chen CY, Chuang WJ. Solution structure and backbone dynamics of the DNA-binding domain of FOXP1: insight into its domain swapping and DNA binding.. Protein Sci 2011 May;20(5):908-24.
- Semina EV, Altherr MR, Murray JC. Cloning and chromosomal localization of two novel human genes encoding LIM-domain binding factors CLIM1 and CLIM2/LDB1/NLI.. Mamm Genome 1998 Nov;9(11):921-4.
- Huentelman MJ, Papassotiropoulos A, Craig DW, Hoerndli FJ, Pearson JV, Huynh KD, Corneveaux J, Hänggi J, Mondadori CR, Buchmann A, Reiman EM, Henke K, de Quervain DJ, Stephan DA. Calmodulin-binding transcription activator 1 (CAMTA1) alleles predispose human episodic memory performance.. Hum Mol Genet 2007 Jun 15;16(12):1469-77.
- Zampieri M, Ciccarone F, Calabrese R, Franceschi C, Bürkle A, Caiafa P. Reconfiguration of DNA methylation in aging.. Mech Ageing Dev 2015 Nov;151:60-70.
- Zhang Z, Deng C, Lu Q, Richardson B. Age-dependent DNA methylation changes in the ITGAL (CD11a) promoter.. Mech Ageing Dev 2002 May;123(9):1257-68.
- Lam LL, Emberly E, Fraser HB, Neumann SM, Chen E, Miller GE, Kobor MS. Factors underlying variable DNA methylation in a human community cohort.. Proc Natl Acad Sci U S A 2012 Oct 16;109 Suppl 2(Suppl 2):17253-60.
- Horvath S, Zhang Y, Langfelder P, Kahn RS, Boks MP, van Eijk K, van den Berg LH, Ophoff RA. Aging effects on DNA methylation modules in human brain and blood tissue.. Genome Biol 2012 Oct 3;13(10):R97.
- Yang X, Han H, De Carvalho DD, Lay FD, Jones PA, Liang G. Gene body methylation can alter gene expression and is a therapeutic target in cancer.. Cancer Cell 2014 Oct 13;26(4):577-90.
- Smurova K, De Wulf P. Centromere and Pericentromere Transcription: Roles and Regulation … in Sickness and in Health.. Front Genet 2018;9:674.
- Foltz DR, Jansen LE, Black BE, Bailey AO, Yates JR 3rd, Cleveland DW. The human CENP-A centromeric nucleosome-associated complex.. Nat Cell Biol 2006 May;8(5):458-69.
- Wang X, Chen D, Gao J, Long H, Zha H, Zhang A, Shu C, Zhou L, Yang F, Zhu B, Wu W. Centromere protein U expression promotes non-small-cell lung cancer cell proliferation through FOXM1 and predicts poor survival.. Cancer Manag Res 2018;10:6971-6984.
- Carter CS, Ramsey MM, Sonntag WE. A critical analysis of the role of growth hormone and IGF-1 in aging and lifespan.. Trends Genet 2002 Jun;18(6):295-301.
- Lee KW, Bogenhagen DF. Assignment of 2'-O-methyltransferases to modification sites on the mammalian mitochondrial large subunit 16 S ribosomal RNA (rRNA).. J Biol Chem 2014 Sep 5;289(36):24936-42.
- Nakamura M, Nishida N, Kawashima M, Aiba Y, Tanaka A, Yasunami M, Nakamura H, Komori A, Nakamuta M, Zeniya M, Hashimoto E, Ohira H, Yamamoto K, Onji M, Kaneko S, Honda M, Yamagiwa S, Nakao K, Ichida T, Takikawa H, Seike M, Umemura T, Ueno Y, Sakisaka S, Kikuchi K, Ebinuma H, Yamashiki N, Tamura S, Sugawara Y, Mori A, Yagi S, Shirabe K, Taketomi A, Arai K, Monoe K, Ichikawa T, Taniai M, Miyake Y, Kumagi T, Abe M, Yoshizawa K, Joshita S, Shimoda S, Honda K, Takahashi H, Hirano K, Takeyama Y, Harada K, Migita K, Ito M, Yatsuhashi H, Fukushima N, Ota H, Komatsu T, Saoshiro T, Ishida J, Kouno H, Kouno H, Yagura M, Kobayashi M, Muro T, Masaki N, Hirata K, Watanabe Y, Nakamura Y, Shimada M, Hirashima N, Komeda T, Sugi K, Koga M, Ario K, Takesaki E, Maehara Y, Uemoto S, Kokudo N, Tsubouchi H, Mizokami M, Nakanuma Y, Tokunaga K, Ishibashi H. Genome-wide association study identifies TNFSF15 and POU2AF1 as susceptibility loci for primary biliary cirrhosis in the Japanese population.. Am J Hum Genet 2012 Oct 5;91(4):721-8.
- Hautefort A, Chesné J, Preussner J, Pullamsetti SS, Tost J, Looso M, Antigny F, Girerd B, Riou M, Eddahibi S, Deleuze JF, Seeger W, Fadel E, Simonneau G, Montani D, Humbert M, Perros F. Pulmonary endothelial cell DNA methylation signature in pulmonary arterial hypertension.. Oncotarget 2017 Aug 8;8(32):52995-53016.
- Marttila S, Kananen L, Häyrynen S, Jylhävä J, Nevalainen T, Hervonen A, Jylhä M, Nykter M, Hurme M. Ageing-associated changes in the human DNA methylome: genomic locations and effects on gene expression.. BMC Genomics 2015 Mar 14;16(1):179.
Citations
This article has been cited 2 times.- Arneson A, Haghani A, Thompson MJ, Pellegrini M, Kwon SB, Vu H, Maciejewski E, Yao M, Li CZ, Lu AT, Morselli M, Rubbi L, Barnes B, Hansen KD, Zhou W, Breeze CE, Ernst J, Horvath S. A mammalian methylation array for profiling methylation levels at conserved sequences.. Nat Commun 2022 Feb 10;13(1):783.
- Horvath S, Haghani A, Peng S, Hales EN, Zoller JA, Raj K, Larison B, Robeck TR, Petersen JL, Bellone RR, Finno CJ. DNA methylation aging and transcriptomic studies in horses.. Nat Commun 2022 Jan 10;13(1):40.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
