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International journal of genomics2019; 2019; 2839614; doi: 10.1155/2019/2839614

Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age.

Abstract: Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanced age of horses is desirable. That is why the identification of aging-related changes in the horse genome is interesting in this respect. In the recent years, the research on aging includes studies of age-related epigenetic effects observed on the DNA methylation level. We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. Genes colocated with age-related differentially methylated regions (age DMRs) are the members of pathways involved in cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression. A positive correlation was found between methylation states and gene expression in particular loci from our data set. Some of described age DMR-linked genes were also reported elsewhere. Obtained results contribute to the knowledge about the molecular basis of aging of equine blood cells.
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Publication Date: 2019-06-02 PubMed ID: 31281827PubMed Central: PMC6589255DOI: 10.1155/2019/2839614Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on examining changes in the horse genome related to aging, particularly by studying the DNA methylation level. Through this study, a number of age-related differential methylation regions (DMRs) in the genomes of blood leukocytes derived from both juvenile and aged horses of the native Hucul breed have been identified.

Objective and Methodology

  • This study aimed to identify the aging-related changes in the horse genome with a particular focus on DNA methylation level.
  • Reduced representation bisulfite sequencing (RRBS) was used as the primary method for the demonstration of age-related DNA methylation regions (DMRs).
  • The species used in the research were juvenile and aged horses from the native Hucul breed.

Findings

  • The researchers found a range of age DMRs in the genomes of blood leukocytes derived from the horses.
  • The genes associated with the age-related DMRs are involved in a variety of physiological processes such as cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression.
  • The researchers identified a positive correlation between methylation states and gene expression in some specific loci from their data set.

Significance

  • The findings of this research contribute to the knowledge about the molecular basis of aging of equine blood cells.
  • By understanding the age-related changes to the horse genome, there is potential to discover new solutions in the prevention and treatment of pathological conditions associated with advanced age in horses.
  • Some of the age DMR-linked genes discovered in this research have also been reported in other studies, further reinforcing their relevance.

Cite This Article

APA
Ząbek T, Semik-Gurgul E, Szmatoła T, Gurgul A, Fornal A, Bugno-Poniewierska M. (2019). Methylation Marks of Blood Leukocytes of Native Hucul Mares Differentiated in Age. Int J Genomics, 2019, 2839614. https://doi.org/10.1155/2019/2839614

Publication

International journal of genomics
ISSN: 2314-4378
NlmUniqueID: 101605206
Country: United States
Language: English
Volume: 2019
Pages: 2839614
PII: 2839614

Researcher Affiliations

Ząbek, T
  • National Research Institute of Animal Production, Balice 32-083, Poland.
Semik-Gurgul, E
  • National Research Institute of Animal Production, Balice 32-083, Poland.
Szmatoła, T
  • National Research Institute of Animal Production, Balice 32-083, Poland.
  • University Centre of Veterinary Medicine, University of Agriculture in Kraków, Al. Mickiewicza 24/28, 30-059 Kraków, Poland.
Gurgul, A
  • National Research Institute of Animal Production, Balice 32-083, Poland.
Fornal, A
  • National Research Institute of Animal Production, Balice 32-083, Poland.
Bugno-Poniewierska, M
  • Institute of Veterinary Sciences, University of Agriculture in Krakow, 31-120, Poland.

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Citations

This article has been cited 3 times.
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