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Home / Equine Research Bank / Cells / Methylation Status of Gene Bodies of Selected microRNA Genes...
Cells2022; 11(12); 1917; doi: 10.3390/cells11121917

Methylation Status of Gene Bodies of Selected microRNA Genes Associated with Neoplastic Transformation in Equine Sarcoids.

Abstract: Horses are of great importance in recreation, livestock production, as working animals in poorly developed countries, and for equine-assisted therapy. Equine sarcoids belong to the most commonly diagnosed tumors in this species. They may cause discomfort, pain, and can lead to the permanent impairment of motor function. The molecular bases of their formation are still under investigation. Our previous studies revealed altered microRNA (miRNA) expression and DNA methylation levels in sarcoid tumors. Abnormal patterns of methylation may be responsible for changes in gene expression levels, including microRNAs. Recently, the DNA methylation of gene bodies has also been shown to have an impact on gene expression. Thus, the aim of the study was to investigate the methylation pattern of gene bodies of chosen miRNAs identified in sarcoid tissue (miR-101, miR-10b, miR-200a, and miR-338-3p), which have also been established to play roles in neoplastic transformation. To this end, we applied qRT-PCR, Bisulfite Sequencing PCR (BSP), and Mquant methods. As a result, we identified the statistically significant downregulation of pri-mir-101-1, pri-mir-10b, and pri-mir-200a in the sarcoid samples in comparison to the control. The DNA methylation analysis revealed their hypermethylation. This suggests that DNA methylation may be one mechanism responsible for the downregulation of theses miRNAs. However, the identified differences in the methylation levels are not very high, which implies that other mechanisms may also underlie the downregulation of the expression of these miRNAs in equine sarcoids. For the first time, the results obtained shed light on microRNA expression regulation by gene body methylation in equine sarcoids and provide bases for further deeper studies on other mechanisms influencing the miRNA repertoire.
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Publication Date: 2022-06-14 PubMed ID: 35741046PubMed Central: PMC9221590DOI: 10.3390/cells11121917Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study explores the impact of DNA methylation on the gene expressions of selected microRNAs in equine sarcoids, common tumors in horses. It suggests that DNA methylation might be one mechanism responsible for reducing microRNA expressions, but other factors might be at play given the slight differences in methylation levels.

Introduction to Equine Sarcoids and DNA Methylation

  • The research’s focus is equine sarcoids, a prevalent type of tumours in horses. These tumors can cause discomfort, pain, and impair motor functions permanently.
  • Despite their prevalence, the molecular basis for the development of these tumors is not fully understood, prompting the need for further research.
  • DNA methylation is one of the mechanisms investigated in this study. DNA methylation refers to a process wherein a methyl group is added to DNA molecules, influencing their functions, notably gene expression. The impact of DNA methylation on gene bodies has recently been discovered to affect gene expression as well.

Study Aim

  • The study aims to understand the patterns of methylation in certain microRNAs (miRNAs)— specifically miR-101, miR-10b, miR-200a, and miR-338-3p— identified in sarcoid tissues. miRNAs are small non-coding RNA molecules that play essential roles in gene expression regulation.
  • These chosen miRNAs are believed to have a significant role in neoplastic transformation— a process where normal cells are transformed into cancerous or tumorous cells.

Methodology

  • The researchers used qRT-PCR, Bisulfite Sequencing PCR (BSP), and Mquant methods to investigate the methylation pattern of the selected miRNAs.

Findings

  • The results showed a significant decrease in the level of pri-mir-101-1, pri-mir-10b, and pri-mir-200a in the sarcoid samples when compared to the controls.
  • DNA methylation analysis revealed an increase in methylation in these microRNAs. This suggests that DNA methylation might be linked to a reduction in these microRNAs’ expression.
  • However, the differences in methylation levels were not significantly high, suggesting other mechanisms could also influence the downregulation of these miRNAs.

Conclusion & Future Direction

  • The study indicates that DNA methylation could be one possible mechanism regulating the expression of microRNA in equine sarcoids. However, the relatively small differences in methylation call for further investigation into other potential underlying mechanisms.
  • This research is a step towards understanding the molecular basis of equine sarcoid formation and may guide future studies on the different mechanisms influencing the microRNA repertoire.

Cite This Article

APA
Pawlina-Tyszko K, Semik-Gurgul E, Ząbek T, Witkowski M. (2022). Methylation Status of Gene Bodies of Selected microRNA Genes Associated with Neoplastic Transformation in Equine Sarcoids. Cells, 11(12), 1917. https://doi.org/10.3390/cells11121917

Publication

Cells
ISSN: 2073-4409
NlmUniqueID: 101600052
Country: Switzerland
Language: English
Volume: 11
Issue: 12
PII: 1917

Researcher Affiliations

Pawlina-Tyszko, Klaudia
  • Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 St., 32-083 Balice, Poland.
Semik-Gurgul, Ewelina
  • Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 St., 32-083 Balice, Poland.
Ząbek, Tomasz
  • Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 St., 32-083 Balice, Poland.
Witkowski, Maciej
  • Institute of Veterinary Medicine, University Centre of Veterinary Medicine JU-AU, Al. Mickiewicza 24/28, 30-059 Cracow, Poland.
  • Horse Clinic Służewiec, Puławska 266 St., 02-684 Warsaw, Poland.

MeSH Terms

  • Animals
  • Cell Transformation, Neoplastic / genetics
  • DNA Methylation / genetics
  • Down-Regulation
  • Gene Expression Regulation, Neoplastic
  • Horses / genetics
  • MicroRNAs / genetics
  • MicroRNAs / metabolism
  • Skin Neoplasms / genetics
  • Skin Neoplasms / metabolism
  • Skin Neoplasms / veterinary

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 1 times.
  1. Semik-Gurgul E, Gurgul A, Szmatoła T. Transcriptome and methylome sequencing reveals altered long non-coding RNA genes expression and their aberrant DNA methylation in equine sarcoids. Funct Integr Genomics 2023 Aug 8;23(3):268.
    doi: 10.1007/s10142-023-01200-2pubmed: 37552338google scholar: lookup
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