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Open veterinary journal2025; 15(8); 3670-3676; doi: 10.5455/OVJ.2025.v15.i8.30

Microbial profile of post-breeding endometritis in Arabian mares from the Al-Hira District, Iraq.

Abstract: in Arabian mares poses a significant reproductive challenge, necessitating proper pathogen identification and antimicrobial susceptibility determination. Unassigned: To investigate the prevalence of intrauterine pathogens and their antimicrobial susceptibility patterns in Arabian mares with postbreeding endometritis. Unassigned: Seventeen Arabian mares were examined clinically and ultrasonographically. Uterine swabs were collected for bacterial isolation and antimicrobial susceptibility testing. Unassigned: Clinical endometritis was detected in 29.4% of the mares. Ultrasonographic examination revealed varying degrees of uterine wall thickness: slight (58.82%), moderate (11.76%), and severe (29.41%). was the predominant pathogen (76.47%), followed by Streptococcus spp. (11.76%), with mixed infections occurring in 11.76% of cases. Both pathogens exhibited high resistance to several antimicrobials but significant susceptibility to β-lactam antibiotics and selected fluoroquinolones. Unassigned: was the primary pathogen associated with postbreeding endometritis in Arabian mares. The high antimicrobial resistance patterns observed emphasize the importance of culture-based treatment selection. β-lactam antibiotics showed promising efficacy against both isolated pathogens.
Publication Date: 2025-08-31 PubMed ID: 41035988PubMed Central: PMC12483455DOI: 10.5455/OVJ.2025.v15.i8.30Google Scholar: Lookup
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  • Journal Article

Cite This Article

APA
Alfatlawy HJ. (2025). Microbial profile of post-breeding endometritis in Arabian mares from the Al-Hira District, Iraq. Open Vet J, 15(8), 3670-3676. https://doi.org/10.5455/OVJ.2025.v15.i8.30

Publication

ISSN: 2218-6050
NlmUniqueID: 101653182
Country: Libya
Language: English
Volume: 15
Issue: 8
Pages: 3670-3676

Researcher Affiliations

Alfatlawy, Hella J
  • Faculty of Veterinary Medicine, University of Kufa, Kufa, Iraq.

MeSH Terms

  • Animals
  • Female
  • Endometritis / veterinary
  • Endometritis / microbiology
  • Endometritis / epidemiology
  • Endometritis / drug therapy
  • Horses
  • Horse Diseases / microbiology
  • Horse Diseases / epidemiology
  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / therapeutic use
  • Escherichia coli / isolation & purification
  • Escherichia coli / drug effects
  • Iraq / epidemiology
  • Microbial Sensitivity Tests / veterinary
  • Escherichia coli Infections / veterinary
  • Escherichia coli Infections / epidemiology
  • Escherichia coli Infections / microbiology
  • Drug Resistance, Bacterial
  • Prevalence
  • Streptococcus / isolation & purification
  • Streptococcus / drug effects

Conflict of Interest Statement

The authors declare no conflicts of interest regarding the publication of this article.

References

This article includes 34 references
  1. Abd-El-Razek E., Genedy T., Elbaz H. Ultrasonographic monitoring and treatment of endometritis in mare. J. Curr. Vet. Res. 2019;1:139–146.
  2. Al-Abidy H.F. Isolation and identification of pathogenic bacteria from genital tract of Arabian mares affected with genital tract infection and antimicrobial sensitivity. Iraqi J. Vet. Sci. 2010;24:143–148.
  3. Albihn A., Båverud V., Magnusson U. Uterine microbiology and antimicrobial susceptibility in isolated bacteria from mares with fertility problems. Acta Vet. Scand. 2003;44:121–129.
    pmc: PMC1831563pubmed: 15074625
  4. Barbary H.A., Abo-ghonema I.I., El-Bawab I.E., Fadel M.S. Diagnosis and treatment of bacterial endometritis in Arabian mares. Alex. J. Vet. Sci. 2016;49:116–125.
  5. Bohn A.A., Ferris R.A., McCue P.M. Comparison of equine endometrial cytology samples collected with uterine swab, uterine brush, and low-volume lavage from healthy mares. Vet. Clin. Pathol. 2014;43:594–600.
    pubmed: 25208767
  6. Canisso I.F., Segabinazzi L.G.T.M., Fedorka C. E. Persistent breeding-induced endometritis in mares - a multifaceted challenge: from clinical aspects to immunopathogenesis and pathobiology. Int. J. Mol. Sci. 2020;21(4):1432. nhttps://doi:10.3390/ijms21041432. n
    pmc: PMC7073041pubmed: 32093296
  7. Canisso I.F., StewBoart J., Coutinho da Silva M.A. Endometritis: managing persistent postbreeding endometritis. Vet. Clin. N. Am. Equine Pract. 2016;32:465–480.
    pubmed: 27810036
  8. Christoffersen M., Woodward E., Bojesen A.M., Jacobsen S., Petersen M.R., Troedsson M.H.T., Lehn-Jensen Inflammatory responses to induced infectious endometritis in mares resistant or susceptible to persistent endometritis. BMC Vet. Res. 2012;8:41–41.
    pmc: PMC3368729pubmed: 22458733
  9. CLSI. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2022. Performance standards for antimicrobial susceptibility testing.
  10. Davis H.A., Stanton M.B., Thungrat K., Boothe D.M. Uterine bacterial isolates from mares and their resistance to antimicrobials: 8296 cases (2003-2008) J. Am. Vet. Med. Assoc. 2013;242:977–983.
    pubmed: 23517211
  11. Ferris R.A., Frisbie D.D., McCue P.M. Use of mesenchymal stem cells or autologous conditioned serum to modulate the inflammatory response to spermatozoa in mares. Theriogenology. 2014;82:36–42.
    pubmed: 24681213
  12. Ginther O.J., Pierson R.A. Ultrasonic anatomy and pathology of the equine uterus. Theriogenology. 1984;21:505–516.
    pubmed: 16725900
  13. Gutjahr S., Paccamonti D.L., Pycock J.F., Taverne M.A., Dieleman S.J., van der Weijden G.C. Effects of treatment dose and day on uterine response to oxytocin in mares. Theriogenology. 2000;54:447–456.
    pubmed: 11051327
  14. Hamouda M.A., Al-Hizab F.A., Ghoneim I.M., Al-Dughaym A.M., Al-Hashim H.J. Assessment of endometritis in Arabian mare. Anim. Prod. 2012;14:99–103.
  15. Kabir A., Lamichhane B., Habib T., Adams A., El-Sheikh Ali H., Slovis N.M., Troedsson M.H.T., Helmy Y.A. Antimicrobial resistance in equines: a growing threat to horse health and beyond-a comprehensive review. Antibiotics. 2024;13:713.
    pmc: PMC11350719pubmed: 39200013
  16. Katila T. Postmating inflammatory responses of the uterus. Reprod. Domest. Anim. 2012;5:31–41.
    pubmed: 22913558
  17. Khan I.U., Khairullah A.R., Khan A.Y., Rehman A.U., Mustofa I. Strategic approaches to improve equine breeding and stud farm outcomes. Vet. World. 2025;18:311–328.
    pmc: PMC11963589pubmed: 40182817
  18. Klein C., Ennen S., Huchzermeyer S., Weiss R., Wehrend A. Analysis of the barrier functions of vulvovaginal fold and cervix to ascending bacterial contamination of the mare’s reproductive tract. Tierarzt. Prax. 2009;80:113–117.
  19. Lambraki I.A., Cousins M., Graells T., Léger A., Henriksson P., Harbarth S. Factors influencing antimicrobial resistance in the European food system and potential leverage points for intervention: a participatory, One Health study. PLoS ONE. 2022;17:e0263914.
    pmc: PMC8863257pubmed: 35192666
  20. LeBlanc M.M. Advances in the diagnosis and treatment of chronic infectious and post-mating-induced endometritis in the mare. Reprod. Domest. Anim. 2010;45:21–27.
    pubmed: 20591061
  21. LeBlanc M.M., Causey R. Clinical and subclinical endometritis in the mare: both threats to fertility. Reprod. Domest. Anim. 2009;44:10–22.
    pubmed: 19660076
  22. Malaluang P., Wilén E., Lindahl J., Hansson I., Morrell J.M. Antimicrobial resistance in equine reproduction. Animals. 2021;11:3035.
    pmc: PMC8614435pubmed: 34827768
  23. Morris H.A., McCue L.M.P., Aurich C. Equine endometritis: a review of challenges and new approaches. Reproduction. 2020;160:R95–R110.
    pubmed: 32805710
  24. Omar H., Hambidge M., Firmanes B., Shabandri A.M., Wilsher S. Bacteria isolated from equine uteri in the United Arab Emirates: a retrospective study. J. Equine Vet. Sci. 2022;115:104029.
    pubmed: 35659620
  25. Portus B.J., Reilas T., Katila T. Effect of seminal plasma on uterine inflammation, contractility and pregnancy rates in mares. Equine Vet. J. 2005;37:515–519.
    pubmed: 16295928
  26. Raidal S.L. Antimicrobial stewardship in equine practice. Aust. Vet. J. 2019;97:238–242.
    pubmed: 31236925
  27. Schöniger S., Schoon H.A. Healthy and diseased equine endometrium: a review of morphological features and molecular analyses. Animals. 2020;10:625.
    pmc: PMC7222714pubmed: 32260515
  28. Traub-Dargatz J.L., Salman M.D., Voss J.L. Medical problems in adult horses as ranked by equine practitioners. J. Am. Vet. Med. Assoc. 1991;198:1745–1747.
    pubmed: 2071472
  29. Troedsson M.H. Breeding-induced endometritis in mares. Vet. Clin. North Am. Equine Pract. 2006;22:705–712.
    pubmed: 17129798
  30. Troedsson M.H.T., Woodward E.M. Our current understanding of the pathophysiology of equine endometritis with an emphasis on breeding-induced endometritis. Reprod. Biol. 2016;16:8–12.
    pubmed: 26952748
  31. Watson E.D. Postbreeding endometritis in the mare. Anim. Reprod. Sci. 2000;60–61:221–232.
    pubmed: 10844197
  32. Wiegand I., Hilpert K., Hancock R.E.W. Agar and broth dilution methods were used to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 2008;3:163–175.
    pubmed: 18274517
  33. Woodward E.M., Troedsson M.H.T. Reproductive tract defense mechanisms in mares. Anim. Reprod. Sci. 2015;47(4):384–389. nhttps://doi:10.1111/evj.12403. n
  34. Zent W.W., Troedsson M.H.T., Xue J.L. Post-breeding uterine fluid accumulation in a normal population of thoroughbred mares: a field study. Am. Ass. Equine Pract. 1998;44:64–65.

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