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Microneutralization test in PK(15) cells for assay of antibodies to louping ill virus.
Abstract: A microneutralization test in PK(15) cells was developed to measure the neutralizing antibody response of a group of ponies experimentally challenged with louping ill virus. Viral cytopathic effect was maximal after 6 days of incubation, at which point titration endpoints were clear-cut and readily determinable. The assay compared favorably with the mouse neutralization test for accuracy and ease of performance.
Publication Date: 1984-07-01 PubMed ID: 6086707PubMed Central: PMC271263DOI: 10.1128/jcm.20.1.128-130.1984Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The study outlines the development of a microneutralization test using PK(15) cells to measure the antibody response of ponies when exposed to the louping ill virus. This method was found to be comparable to the mouse neutralization test for accuracy and ease of application.
Development of Microneutralization Test in PK(15) Cells
- The research primarily focuses on the development of a microneutralization test using Porcine Kidney 15 (PK15) cells.
- This novel approach was designed to measure the neutralizing antibody response when ponies were experimentally introduced to the louping ill virus.
Experiment Process and Results
- Throughout the course of the experiment, it was noted that the viral cytopathic effect was at its highest after six days of incubation.
- It was at this point that the titration endpoints became clearly distinguishable and could be readily determined.
- The scientists were then able to measure and determine the level of neutralizing antibodies produced by the ponies in response to the louping ill virus.
Comparison with Mouse Neutralization Test
- The developed PK(15) cell-based microneutralization test was then compared with the traditional mouse neutralization test.
- In terms of accuracy and ease of performance, the newly developed test proved to be comparable.
- This conclusion suggests that this novel testing method can be as effective as the conventional mouse neutralization test, potentially expanding research options in this area.
Cite This Article
APA
Timoney PJ, Geraghty VP, Harrington AM, Dillon PB.
(1984).
Microneutralization test in PK(15) cells for assay of antibodies to louping ill virus.
J Clin Microbiol, 20(1), 128-130.
https://doi.org/10.1128/jcm.20.1.128-130.1984 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antibodies, Viral / analysis
- Cell Line
- Cytopathogenic Effect, Viral
- Encephalitis Viruses, Tick-Borne / immunology
- Horses / immunology
- Neutralization Tests
References
This article includes 12 references
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- De Madrid AT, Porterfield JS. A simple micro-culture method for the study of group B arboviruses.. Bull World Health Organ 1969;40(1):113-21.
- Reid HW, Doherty PC. Louping-ill encephalomyelitis in the sheep. I. The relationship of viraemia and the antibody response to susceptibility.. J Comp Pathol 1971 Oct;81(4):521-9.
- Pantuwatana S, Thompson WH, Watts DM, Hanson RP. Experimental infection of chipmunks and squirrels with La Crosse and Trivittatus viruses and biological transmission of La Crosse virus by Aedes triseriatus.. Am J Trop Med Hyg 1972 Jul;21(4):476-81.
- PORTERFIELD JS. A simple plaque-inhibition test for the study of arthropod-borne viruses.. Bull World Health Organ 1960;22(3-4):373-80.
- Timoney PJ. Susceptibility of the horse to experimental inoculation with louping ill virus.. J Comp Pathol 1980 Jan;90(1):73-86.
- SMITHBURN KC, KERR JA, GATNE PB. Neutralizing antibodies against certain viruses in the sera of residents of India.. J Immunol 1954 Apr;72(4):248-57.
- WILLIAMS HE. Growth and titration of louping-ill virus in monolayer tissue culture of pig kidney.. Nature 1958 Feb 15;181(4607):497-8.
- SMITHBURN KC, KOKERNOT RH, HEYMANN CS, WEINBREN MP, ZENTKOWSKY D. Neutralizing antibodies for certain viruses in the sera of human beings residing in Northern Natal.. S Afr Med J 1959 Jul 4;33(27):555-61.
- KAARIAINEN L, HALONEN P. Metabolic inhibition test for louping-ill, measles and herpes simplex virus in plastic panels.. Arch Gesamte Virusforsch 1961;11:428-39.
- Timoney PJ, Donnelly WC, Clements C, Fenlon M. Letter: louping ill infection in the horse.. Vet Rec 1974 Dec 7;95(23):540.
Citations
This article has been cited 2 times.- Kang X, Yang BA, Hu Y, Zhao H, Xiong W, Yang Y, Si B, Zhu Q. Human neutralizing Fab molecules against severe acute respiratory syndrome coronavirus generated by phage display. Clin Vaccine Immunol 2006 Aug;13(8):953-7.
- Popuche D, Huaman A, Loyola S, Silva M, Jenkins SA, Guevara C. Development and validation of a vero cell-based suspension method for the detection of Zika virus. Rev Peru Med Exp Salud Publica 2023 Jul-Sep;40(3):297-306.
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