English
MicroRNA-140 expression during chondrogenic differentiation of equine cord blood-derived mesenchymal stromal cells.
Abstract: MicroRNAs are a class of short noncoding RNAs that are involved in various biological processes, including differentiation. MicroRNA-140 (miR-140) has been identified as a cartilage-specific microRNA with several targets involved in cartilage development and homeostasis. The aim of this study was to investigate the expression of miR-140 during chondrogenic differentiation of equine cord blood-derived mesenchymal stromal cells (eCB-MSCs). We demonstrate both that miR-140 is highly expressed in normal equine articular cartilage and that eCB-MSCs express significantly higher levels of this microRNA after 14 days of chondrogenic differentiation. Furthermore, miR-140 expression closely paralleled that of the cartilage-specific transcription factor Sox9, suggesting that miR-140 may be under the transcriptional regulation of Sox9 in these cells. The expression patterns of miR-140 targets the chemokine (CXC motif) ligand 12 (CXCL12), A disintegrin and metalloproteinase with thrombosponin motifs (ADAMTS)-5 and insulin growth factor binding protein 5 (IGFBP5) were also determined; however, only CXCL12 and ADAMTS-5 were repressed while miR-140 expression was upregulated. Together, these studies suggest that miR-140 is an important regulator of cartilage development and homeostasis in eCB-MSCs that may act, in part, through the regulation of CXCL12 and ADAMTS-5.
Publication Date: 2013-01-15
PubMed ID: 23157248DOI: 10.1089/scd.2012.0411Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article describes an investigation into the role of MicroRNA-140 (miR-140) during the chondrogenic (cartilage forming) differentiation of equine cord blood-derived mesenchymal stromal cells (eCB-MSCs). The study found that miR-140, a cartilage-specific microRNA, is highly expressed in normal equine articular cartilage and its expression rises significantly in eCB-MSCs after two weeks of chondrogenic differentiation.
About MicroRNAs
- MicroRNAs are a class of short noncoding RNAs — i.e., sequences of RNA that do not code for proteins. They’re involved in a range of biological processes, including cellular differentiation — the process in which unspecialized cells mature into their specialized forms.
- MicroRNA-140, in particular, is noted as a cartilage-specific microRNA. This means it plays a major role in the development and maintenance (homeostasis) of cartilage.
The Study and Its Findings
- The study’s goal was to explore how miR-140 behaves during the chondrogenic differentiation of eCB-MSCs — essentially, when these cells were made to develop into cartilage cells.
- The researchers found that miR-140 is present in high quantities in healthy equine articular cartilage (the cartilage found in joints).
- More importantly, when they induced chondrogenic differentiation in eCB-MSCs, the cells showed significantly increased levels of miR-140 expression after 14 days. This finding suggests a crucial role for miR-140 in cartilage formation.
- The researchers also found that miR-140’s expression pattern closely matched that of Sox9, a transcription factor known to be specific to cartilage. This finding suggests that Sox9 may control miR-140’s expression in these cells.
Regulation of miR-140 Targets
- Additionally, the researchers examined how the miR-140 expression pattern affected the expression of its various targets, such as chemokine CXCL12, ADAMTS-5, and IGFBP5. These targets have roles in cartilage development and homeostasis.
- While all three targets were expected to be downregulated (repressed) with the upregulation of miR-140, only CXCL12 and ADAMTS-5 behaved as expected, suggesting miR-140 regulates these targets during chondrogenic differentiation.
Implications of the Study
- Together, these results propose that miR-140 plays a significant role in the development and maintenance of cartilage in eCB-MSCs. The discovery furthers our understanding of cartilage growth and could potentially assist in devising effective therapies for diseases that involve cartilage degradation, like osteoarthritis.
Cite This Article
APA
Buechli ME, Lamarre J, Koch TG.
(2013).
MicroRNA-140 expression during chondrogenic differentiation of equine cord blood-derived mesenchymal stromal cells.
Stem Cells Dev, 22(8), 1288-1296.
https://doi.org/10.1089/scd.2012.0411
Publication
Researcher Affiliations
- Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
MeSH Terms
- ADAM Proteins / genetics
- ADAM Proteins / metabolism
- Aggrecans / genetics
- Aggrecans / metabolism
- Animals
- Cartilage, Articular / cytology
- Cell Differentiation / genetics
- Cells, Cultured
- Chemokine CXCL12 / genetics
- Chemokine CXCL12 / metabolism
- Chondrocytes / cytology
- Chondrocytes / metabolism
- Chondrogenesis / genetics
- Collagen / genetics
- Collagen / metabolism
- Fetal Blood / cytology
- Gene Expression
- Horses
- Immunohistochemistry
- Insulin-Like Growth Factor Binding Protein 5 / genetics
- Insulin-Like Growth Factor Binding Protein 5 / metabolism
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / metabolism
- MicroRNAs / genetics
- Reverse Transcriptase Polymerase Chain Reaction
- SOX9 Transcription Factor / genetics
- SOX9 Transcription Factor / metabolism
- Time Factors
Citations
This article has been cited 18 times.- Hu WS, Zhang Q, Li SH, Ai SC, Wu QF. Ten Hotspot MicroRNAs and Their Potential Targets of Chondrocytes Were Revealed in Osteoarthritis Based on Bibliometric Analysis.. J Healthc Eng 2022;2022:8229148.
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