Microsatellite markers for evaluating the diversity of the natural killer complex and major histocompatibility complex genomic regions in domestic horses.
Abstract: Genotyping microsatellite markers represents a standard, relatively easy, and inexpensive method of assessing genetic diversity of complex genomic regions in various animal species, such as the major histocompatibility complex (MHC) and/or natural killer cell receptor (NKR) genes. MHC-linked microsatellite markers have been identified and some of them were used for characterizing MHC polymorphism in various species, including horses. However, most of those were MHC class II markers, while MHC class I and III sub-regions were less well covered. No tools for studying genetic diversity of NKR complex genomic regions are available in horses. Therefore, the aims of this work were to establish a panel of markers suitable for analyzing genetic diversity of the natural killer complex (NKC), and to develop additional microsatellite markers of the MHC class I and class III genomic sub-regions in horses. Nine polymorphic microsatellite loci were newly identified in the equine NKC. Along with two previously reported microsatellites flanking this region, they constituted a panel of 11 loci allowing to characterize genetic variation in this functionally important part of the horse genome. Four newly described MHC class I/III-linked markers were added to 11 known microsatellites to establish a panel of 15 MHC markers with a better coverage of the class I and class III sub-regions. Major characteristics of the two panels produced on a group of 65 horses of 13 breeds and on five Przewalski's horses showed that they do reflect genetic variation within the horse species.
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
Publication Date: 2018-02-21 PubMed ID: 29341455DOI: 10.1111/tan.13211Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article focuses on genotyping microsatellite markers to assess the genetic diversity of complex genomic regions in domestic horses, particularly within the major histocompatibility complex (MHC) and natural killer cell receptor (NKR) genes. The study establishes a panel of markers for the natural killer complex and further develops microsatellite markers for the MHC class I and III genomic sub-regions in horses.
Objective
The purpose of the study was twofold: to develop a set of markers that could accurately analyze the genetic diversity within the natural killer complex (NKC) in horses and to create additional microsatellite markers for the sub-regions MHC class I and class III which had previously been underrepresented in the genotyping pool.
Methods
- Nine new polymorphic microsatellite loci were identified in the equine NKC. These new markers, along with two previously reported markers related to this region, formed a panel of 11 loci that allowed for a more in-depth characterization of the genetic variation within this part of the horse genome.
- Four novel MHC class I/III-linked markers were added to the 11 known microsatellites to construct a comprehensive panel of 15 MHC markers that covered more extensively the class I and III sub-regions.
- The researchers tested the two marker panels on a group of 65 horses from 13 different breeds as well as on five Przewalski’s horses, an endangered wild horse breed.
Results
- The study confirmed that the newly developed panels of markers were capable of characterizing genetic variation within the horse species, specifically in the NKC genomic region and MHC class I and III sub-regions.
- This improved understanding of genetic diversity in horses may assist in the ongoing management of horse breeds and in further breeding strategies.
Significance
The research is significant as it demonstrates the ability of genotyping microsatellite markers to provide a high-resolution assessment of the genetic diversity in the complex genomic regions of domestic horses. The newly identified markers offer an expanded understanding of the genetic foundation of these regions and enable an increased capability for ongoing research and breed management in equine species.
Cite This Article
APA
Horecky C, Horecka E, Futas J, Janova E, Horin P, Knoll A.
(2018).
Microsatellite markers for evaluating the diversity of the natural killer complex and major histocompatibility complex genomic regions in domestic horses.
HLA, 91(4), 271-279.
https://doi.org/10.1111/tan.13211 Publication
Researcher Affiliations
- Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
- CEITEC-MENDELU, Mendel University in Brno, Brno, Czech Republic.
- Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
- CEITEC-MENDELU, Mendel University in Brno, Brno, Czech Republic.
- Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
- CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
- Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
- CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
- Department of Animal Genetics, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
- CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences Brno, Brno, Czech Republic.
- Department of Animal Morphology, Physiology and Genetics, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic.
- CEITEC-MENDELU, Mendel University in Brno, Brno, Czech Republic.
MeSH Terms
- Animals
- Animals, Domestic / genetics
- Breeding
- Genetic Loci
- Genetic Variation
- Genome
- Horses / genetics
- Killer Cells, Natural / metabolism
- Major Histocompatibility Complex / genetics
- Microsatellite Repeats / genetics
Citations
This article has been cited 3 times.- Knoll A, Wijacki J, Plasil M, Burger PA, Horin P. Microsatellite markers of the major histocompatibility complex genomic region of domestic camels.. Front Genet 2022;13:1015288.
- Jaworska J, Ropka-Molik K, Wocławek-Potocka I, Siemieniuch M. Inter- and intrabreed diversity of the major histocompatibility complex (MHC) in primitive and draft horse breeds.. PLoS One 2020;15(2):e0228658.
- Stejskalova K, Janova E, Horecky C, Horecka E, Vaclavek P, Hubalek Z, Relling K, Cvanova M, D'Amico G, Mihalca AD, Modry D, Knoll A, Horin P. Associations between the presence of specific antibodies to the West Nile Virus infection and candidate genes in Romanian horses from the Danube delta.. Mol Biol Rep 2019 Aug;46(4):4453-4461.
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