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Journal, genetic engineering & biotechnology2017; 15(2); 469-474; doi: 10.1016/j.jgeb.2017.06.004

Mitochondrial DNA genetic variations among four horse populations in Egypt.

Abstract: Horses are one of the early domesticated animals in the world that changed societies and civilizations on a continent-wide scale. Due to the rare information about the genetic characterization of different horse populations in Egypt, this study aimed to identify the genetic biodiversity and relationships between four horse populations reared in Egypt. Genomic DNA was extracted and mtDNA region was amplified using polymerase chain reaction (PCR). The alignment of 384-bp amplified fragments showed the presence of 41 polymorphic sites resulting in 29 haplotypes which their sequences were submitted to GenBank under the accession numbers: -. The phylogeny tree for tested horses declared the presence of mixing maternal lineages between the four tested populations but still there are some separated lineages especially for Arabian and Thoroughbred horses. The sequences of 72 tested sequences were aligned with 13 published sequences as references, 11 of them for different whereas the other two reference sequences for and . The results showed that all tested horses from the four populations are grouped with reference sequences of and separated from the other two reference sequences of and . It is concluded that sequence analysis of mtDNA control region is still the most informative tool for the identification of genetic biodiversity and phylogeny of different horse breeds and populations. The horse populations reared in Egypt possess low genetic diversity and all of them are belonged to breed.
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Publication Date: 2017-07-06 PubMed ID: 30647688PubMed Central: PMC6296616DOI: 10.1016/j.jgeb.2017.06.004Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studied the genetic biodiversity and relationships among four horse populations in Egypt using the technique of mitochondrial DNA (mtDNA) extraction and Polymerase Chain Reaction (PCR) amplification. The results showed a mix of maternal lineages among the populations but also some distinct lineages, particularly for Arabian and Thoroughbred horses.

Understanding the Research Process

  • The study was conducted with the intent of recognizing the genetic characteristics of various horse populations in Egypt due to a lack of existing data.
  • Scientists extracted genomic DNA from the horses and amplified the mitochondrial DNA region using a process known as Polymerase Chain Reaction (PCR).
  • After the DNA was amplified, the scientists found the presence of 41 polymorphic sites which resulted in 29 haplotypes. These sequences were uploaded to GenBank for future reference.

Assessing Genetic Diversity and Relationships

  • The study findings showed that there’s a mix of maternal lineages among the four horse populations tested. However, some unique lineages were found, particularly in Arabian and Thoroughbred horses, indicating the existence of genetic diversity.
  • The sequences of the horses tested were compared with 13 already published references. This comparison revealed that all of the tested horses from the four populations aligned with certain reference sequences, thus separating them from other reference sequences.
  • The conclusion drawn from this research is that sequence analysis of the mtDNA control region remains a highly informative method for identifying genetic biodiversity and lineage amongst different horse breeds and populations.

Implications on Horse Diversity in Egypt

  • The study found that the horse populations reared in Egypt have a relatively low genetic diversity. This might be due to the fact that they all belong to one breed, despite having a variety of lineages.
  • The findings of this study could serve as valuable data in the future to ensure the diversity of horse populations in Egypt. They can guide breeding plans and contribute to the preservation and enhancement of the genetic basis of Egypt’s horse populations.

Cite This Article

APA
Othman OE, Mahrous KF, Shafey HI. (2017). Mitochondrial DNA genetic variations among four horse populations in Egypt. J Genet Eng Biotechnol, 15(2), 469-474. https://doi.org/10.1016/j.jgeb.2017.06.004

Publication

Journal, genetic engineering & biotechnology
ISSN: 2090-5920
NlmUniqueID: 101317150
Country: Netherlands
Language: English
Volume: 15
Issue: 2
Pages: 469-474

Researcher Affiliations

Othman, Othman E
  • Cell Biology Department, National Research Centre, Dokki, Egypt.
Mahrous, Karima F
  • Cell Biology Department, National Research Centre, Dokki, Egypt.
Shafey, Heba I
  • Cell Biology Department, National Research Centre, Dokki, Egypt.

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Citations

This article has been cited 6 times.
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