FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / J Genet Eng Biotechnol / Mitochondrial DNA genetic variations among four horse popula...
Journal, genetic engineering & biotechnology2017; 15(2); 469-474; doi: 10.1016/j.jgeb.2017.06.004

Mitochondrial DNA genetic variations among four horse populations in Egypt.

Abstract: Horses are one of the early domesticated animals in the world that changed societies and civilizations on a continent-wide scale. Due to the rare information about the genetic characterization of different horse populations in Egypt, this study aimed to identify the genetic biodiversity and relationships between four horse populations reared in Egypt. Genomic DNA was extracted and mtDNA region was amplified using polymerase chain reaction (PCR). The alignment of 384-bp amplified fragments showed the presence of 41 polymorphic sites resulting in 29 haplotypes which their sequences were submitted to GenBank under the accession numbers: -. The phylogeny tree for tested horses declared the presence of mixing maternal lineages between the four tested populations but still there are some separated lineages especially for Arabian and Thoroughbred horses. The sequences of 72 tested sequences were aligned with 13 published sequences as references, 11 of them for different whereas the other two reference sequences for and . The results showed that all tested horses from the four populations are grouped with reference sequences of and separated from the other two reference sequences of and . It is concluded that sequence analysis of mtDNA control region is still the most informative tool for the identification of genetic biodiversity and phylogeny of different horse breeds and populations. The horse populations reared in Egypt possess low genetic diversity and all of them are belonged to breed.
Get Full Text
Publication Date: 2017-07-06 PubMed ID: 30647688PubMed Central: PMC6296616DOI: 10.1016/j.jgeb.2017.06.004Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article studied the genetic biodiversity and relationships among four horse populations in Egypt using the technique of mitochondrial DNA (mtDNA) extraction and Polymerase Chain Reaction (PCR) amplification. The results showed a mix of maternal lineages among the populations but also some distinct lineages, particularly for Arabian and Thoroughbred horses.

Understanding the Research Process

  • The study was conducted with the intent of recognizing the genetic characteristics of various horse populations in Egypt due to a lack of existing data.
  • Scientists extracted genomic DNA from the horses and amplified the mitochondrial DNA region using a process known as Polymerase Chain Reaction (PCR).
  • After the DNA was amplified, the scientists found the presence of 41 polymorphic sites which resulted in 29 haplotypes. These sequences were uploaded to GenBank for future reference.

Assessing Genetic Diversity and Relationships

  • The study findings showed that there’s a mix of maternal lineages among the four horse populations tested. However, some unique lineages were found, particularly in Arabian and Thoroughbred horses, indicating the existence of genetic diversity.
  • The sequences of the horses tested were compared with 13 already published references. This comparison revealed that all of the tested horses from the four populations aligned with certain reference sequences, thus separating them from other reference sequences.
  • The conclusion drawn from this research is that sequence analysis of the mtDNA control region remains a highly informative method for identifying genetic biodiversity and lineage amongst different horse breeds and populations.

Implications on Horse Diversity in Egypt

  • The study found that the horse populations reared in Egypt have a relatively low genetic diversity. This might be due to the fact that they all belong to one breed, despite having a variety of lineages.
  • The findings of this study could serve as valuable data in the future to ensure the diversity of horse populations in Egypt. They can guide breeding plans and contribute to the preservation and enhancement of the genetic basis of Egypt’s horse populations.

Cite This Article

APA
Othman OE, Mahrous KF, Shafey HI. (2017). Mitochondrial DNA genetic variations among four horse populations in Egypt. J Genet Eng Biotechnol, 15(2), 469-474. https://doi.org/10.1016/j.jgeb.2017.06.004

Publication

Journal, genetic engineering & biotechnology
ISSN: 2090-5920
NlmUniqueID: 101317150
Country: Netherlands
Language: English
Volume: 15
Issue: 2
Pages: 469-474

Researcher Affiliations

Othman, Othman E
  • Cell Biology Department, National Research Centre, Dokki, Egypt.
Mahrous, Karima F
  • Cell Biology Department, National Research Centre, Dokki, Egypt.
Shafey, Heba I
  • Cell Biology Department, National Research Centre, Dokki, Egypt.

References

This article includes 32 references
  1. Anthony D.W., Bogucki P., Coms E., Gimbutas M., Jovanovic B. Curr. Anthropol. 1986;27(4):291–313.
  2. Achilli A, Olivieri A, Soares P, Lancioni H, Hooshiar Kashani B, Perego UA, Nergadze SG, Carossa V, Santagostino M, Capomaccio S, Felicetti M, Al-Achkar W, Penedo MC, Verini-Supplizi A, Houshmand M, Woodward SR, Semino O, Silvestrelli M, Giulotto E, Pereira L, Bandelt HJ, Torroni A. Mitochondrial genomes from modern horses reveal the major haplogroups that underwent domestication.. Proc Natl Acad Sci U S A 2012 Feb 14;109(7):2449-54.
    pmc: PMC3289334pubmed: 22308342doi: 10.1073/pnas.1111637109google scholar: lookup
  3. Bowling A, Ruvinsky A. Genetic Aspects of Domestication. .
  4. Cardinali I, Lancioni H, Giontella A, Capodiferro MR, Capomaccio S, Buttazzoni L, Biggio GP, Cherchi R, Albertini E, Olivieri A, Cappelli K, Achilli A, Silvestrelli M. An Overview of Ten Italian Horse Breeds through Mitochondrial DNA.. PLoS One 2016;11(4):e0153004.
    pmc: PMC4824442pubmed: 27054850doi: 10.1371/journal.pone.0153004google scholar: lookup
  5. Cieslak M, Pruvost M, Benecke N, Hofreiter M, Morales A, Reissmann M, Ludwig A. Origin and history of mitochondrial DNA lineages in domestic horses.. PLoS One 2010 Dec 20;5(12):e15311.
    pmc: PMC3004868pubmed: 21187961doi: 10.1371/journal.pone.0015311google scholar: lookup
  6. Cunningham EP, Dooley JJ, Splan RK, Bradley DG. Microsatellite diversity, pedigree relatedness and the contributions of founder lineages to thoroughbred horses.. Anim Genet 2001 Dec;32(6):360-4.
    pubmed: 11736806doi: 10.1046/j.1365-2052.2001.00785.xgoogle scholar: lookup
  7. Czerneková V., Kott T., Majzlík I. Czech J. Anim. Sci. 2013;58(10):437–442.
  8. Głażewska I. Livest. Sci. 2010;129(1–3):49–55.
  9. Gray MW, Burger G, Lang BF. Mitochondrial evolution.. Science 1999 Mar 5;283(5407):1476-81.
    pubmed: 10066161doi: 10.1126/science.283.5407.1476google scholar: lookup
  10. Guastella AM, Zuccaro A, Criscione A, Marletta D, Bordonaro S. Genetic analysis of Sicilian autochthonous horse breeds using nuclear and mitochondrial DNA markers.. J Hered 2011 Nov-Dec;102(6):753-8.
    pubmed: 21914666doi: 10.1093/jhered/esr091google scholar: lookup
  11. Gus Cothran E., Juras R., Macijauskiene V. Genet. Mol. Biol. 2005;28(4):677–681.
  12. Hall T.A. Nucl. Acids Symp. Ser. 1999;41:95–98.
  13. Hill EW, Bradley DG, Al-Barody M, Ertugrul O, Splan RK, Zakharov I, Cunningham EP. History and integrity of thoroughbred dam lines revealed in equine mtDNA variation.. Anim Genet 2002 Aug;33(4):287-94.
    pubmed: 12139508doi: 10.1046/j.1365-2052.2002.00870.xgoogle scholar: lookup
  14. Hutchison CA 3rd, Newbold JE, Potter SS, Edgell MH. Maternal inheritance of mammalian mitochondrial DNA.. Nature 1974 Oct 11;251(5475):536-8.
    pubmed: 4423884doi: 10.1038/251536a0google scholar: lookup
  15. Ishida N, Hasegawa T, Takeda K, Sakagami M, Onishi A, Inumaru S, Komatsu M, Mukoyama H. Polymorphic sequence in the D-loop region of equine mitochondrial DNA.. Anim Genet 1994 Aug;25(4):215-21.
    pubmed: 7985837doi: 10.1111/j.1365-2052.1994.tb00196.xgoogle scholar: lookup
  16. Jansen T, Forster P, Levine MA, Oelke H, Hurles M, Renfrew C, Weber J, Olek K. Mitochondrial DNA and the origins of the domestic horse.. Proc Natl Acad Sci U S A 2002 Aug 6;99(16):10905-10.
    pmc: PMC125071pubmed: 12130666doi: 10.1073/pnas.152330099google scholar: lookup
  17. Khanshour AM, Cothran EG. Maternal phylogenetic relationships and genetic variation among Arabian horse populations using whole mitochondrial DNA D-loop sequencing.. BMC Genet 2013 Sep 13;14:83.
    pmc: PMC3847362pubmed: 24034565doi: 10.1186/1471-2156-14-83google scholar: lookup
  18. Kim KI, Yang YH, Lee SS, Park C, Ma R, Bouzat JL, Lewin HA. Phylogenetic relationships of Cheju horses to other horse breeds as determined by mtDNA D-loop sequence polymorphism.. Anim Genet 1999 Apr;30(2):102-8.
    pubmed: 10376300doi: 10.1046/j.1365-2052.1999.00419.xgoogle scholar: lookup
  19. Lei CZ, Su R, Bower MA, Edwards CJ, Wang XB, Weining S, Liu L, Xie WM, Li F, Liu RY, Zhang YS, Zhang CM, Chen H. Multiple maternal origins of native modern and ancient horse populations in China.. Anim Genet 2009 Dec;40(6):933-44.
    pubmed: 19744143doi: 10.1111/j.1365-2052.2009.01950.xgoogle scholar: lookup
  20. Levine MA. Late Prehistoric Exploitation of the Eurasian Steppe. Cambridge McDonald Institute, 1999.
  21. Levine MA. The Domestication of horse: the origins, development and management of its behavior. Cambridge University Press, 2005.
  22. Librado P, Rozas J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data.. Bioinformatics 2009 Jun 1;25(11):1451-2.
    pubmed: 19346325doi: 10.1093/bioinformatics/btp187google scholar: lookup
  23. Lira J, Linderholm A, Olaria C, Brandström Durling M, Gilbert MT, Ellegren H, Willerslev E, Lidén K, Arsuaga JL, Götherström A. Ancient DNA reveals traces of Iberian Neolithic and Bronze Age lineages in modern Iberian horses.. Mol Ecol 2010 Jan;19(1):64-78.
    pubmed: 19943892doi: 10.1111/j.1365-294x.2009.04430.xgoogle scholar: lookup
  24. Luis C., Bastos-Silveira C., Cothran E.G., Oom M.M. Genet. Mol. Biol. 2002;25:309–311.
  25. Mahrous K.F., Hassanane M., Abdel Mordy M., Shafey H., Hassan N. Genet. Eng. Biotech. 2011;9(2):103–109.
  26. Meadows JR, Hanotte O, Drögemüller C, Calvo J, Godfrey R, Coltman D, Maddox JF, Marzanov N, Kantanen J, Kijas JW. Globally dispersed Y chromosomal haplotypes in wild and domestic sheep.. Anim Genet 2006 Oct;37(5):444-53.
    pubmed: 16978172doi: 10.1111/j.1365-2052.2006.01496.xgoogle scholar: lookup
  27. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells.. Nucleic Acids Res 1988 Feb 11;16(3):1215.
    pmc: PMC334765pubmed: 3344216doi: 10.1093/nar/16.3.1215google scholar: lookup
  28. Mirol PM, Peral García P, Vega-Pla JL, Dulout FN. Phylogenetic relationships of Argentinean Creole horses and other South American and Spanish breeds inferred from mitochondrial DNA sequences.. Anim Genet 2002 Oct;33(5):356-63.
    pubmed: 12354144doi: 10.1046/j.1365-2052.2002.00884.xgoogle scholar: lookup
  29. Sutovsky P, Schatten G. Paternal contributions to the mammalian zygote: fertilization after sperm-egg fusion.. Int Rev Cytol 2000;195:1-65.
    pubmed: 10603574doi: 10.1016/s0074-7696(08)62703-5google scholar: lookup
  30. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.. Mol Biol Evol 2011 Oct;28(10):2731-9.
    pmc: PMC3203626pubmed: 21546353doi: 10.1093/molbev/msr121google scholar: lookup
  31. Xu X., Arnason U. Gene. 1994;148:657–662.
  32. Zechner P., Solkner J., Bodo I., Druml T., Baumung R., Achmann R., Marti E., Habe F., Brem G. Livest. Prod. Sci. 2002;77(2–3):137–146.

Citations

This article has been cited 6 times.
  1. Mund SJK, MacPhee DJ, Campbell J, Honaramooz A, Wobeser B, Barber SM. Macroscopic, Histologic, and Immunomodulatory Response of Limb Wounds Following Intravenous Allogeneic Cord Blood-Derived Multipotent Mesenchymal Stromal Cell Therapy in Horses. Cells 2021 Nov 1;10(11).
    doi: 10.3390/cells10112972pubmed: 34831196google scholar: lookup
  2. Mund SJK, Kawamura E, Awang-Junaidi AH, Campbell J, Wobeser B, MacPhee DJ, Honaramooz A, Barber S. Homing and Engraftment of Intravenously Administered Equine Cord Blood-Derived Multipotent Mesenchymal Stromal Cells to Surgically Created Cutaneous Wound in Horses: A Pilot Project. Cells 2020 May 8;9(5).
    doi: 10.3390/cells9051162pubmed: 32397125google scholar: lookup
  3. Kvist L, Niskanen M, Mannermaa K, Wutke S, Aspi J. Genetic variability and history of a native Finnish horse breed. Genet Sel Evol 2019 Jul 1;51(1):35.
    doi: 10.1186/s12711-019-0480-8pubmed: 31262246google scholar: lookup
  4. Agbani A, Aminou O, Machmoum M, Germot A, Badaoui B, Petit D, Piro M. A Systematic Literature Review of Mitochondrial DNA Analysis for Horse Genetic Diversity. Animals (Basel) 2025 Mar 20;15(6).
    doi: 10.3390/ani15060885pubmed: 40150414google scholar: lookup
  5. Du W, Sun Q, Hu S, Yu P, Kan S, Zhang W. Equus mitochondrial pangenome reveals independent domestication imprints in donkeys and horses. Sci Rep 2025 Feb 25;15(1):6803.
    doi: 10.1038/s41598-025-91564-1pubmed: 40000832google scholar: lookup
  6. Khaudov AD, Duduev AS, Kokov ZA, Amshokov KK, Zhekamukhov MK, Zaitsev AM, Reissmann M. Genetic analysis of maternal and paternal lineages in Kabardian horses by uniparental molecular markers. Open Vet J 2018;8(1):40-46.
    doi: 10.4314/ovj.v8i1.7pubmed: 29445620google scholar: lookup
Subscribe to our newsletter
Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.