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Home / Equine Research Bank / J Mol Evol / Mitochondrial DNA sequences of various species of the genus ...
Journal of molecular evolution1995; 41(2); 180-188; doi: 10.1007/BF00170671

Mitochondrial DNA sequences of various species of the genus Equus with special reference to the phylogenetic relationship between Przewalskii’s wild horse and domestic horse.

Abstract: The noncoding region between tRNAPro and the large conserved sequence block is the most variable region in the mammalian mitochondrial DNA D-loop region. This variable region (ca. 270 bp) of four species of Equus, including Mongolian and Japanese native domestic horses as well as Przewalskii's (or Mongolian) wild horse, were sequenced. These data were compared with our recently published Thoroughbred horse mitochondrial DNA sequences. The evolutionary rate of this region among the four species of Equus was estimated to be 2-4 x 10(-8) per site per year. Phylogenetic trees of Equus species demonstrate that Przewalskii's wild horse is within the genetic variation among the domestic horse. This suggests that the chromosome number change (probably increase) of the Przewalskii's wild horse occurred rather recently.
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Publication Date: 1995-08-01 PubMed ID: 7666447DOI: 10.1007/BF00170671Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines the DNA sequences from various species, particularly the wild and domestic horses. It reveals that Przewalskii’s wild horse shares genetic variation with the domestic horse, suggesting a recent change in the wild horse’s chromosome number.

Introduction and DNA Selection

  • The science team focused on a region of DNA known as the noncoding area, which lies between tRNAPro and a large conserved sequence block. This noncoding region doesn’t code for specific proteins, but it plays a vital role in controlling genes and influencing how they behave.
  • This specific noncoding region, approximately 270 base pairs long, is the most variable within the mammalian mitochondrial DNA D-loop region. This means it fluctuates more than other regions, making it perfect for studying genetic variation and evolution.
  • The species selected for this study were various species from the Equus genus, including both Mongolian and Japanese native domestic horses, and Przewalskii’s wild horse.

Sequence Analysis and Comparisons

  • The team sequenced this region from the selected horse species and compared the obtained data with previously analyzed DNA sequences from Thoroughbred horses.
  • They used this information to estimate the rate of evolutionary changes in this specific region among the four horse species sampled. The calculated rate was between 2-4 x 10(-8) changes per genomic site per year.

Phylogenetics and Interpretation

  • Phylogenetic trees were constructed with these data, providing a visual map of the genetic relationships between the species within the Equus genus. It helped track and map the evolution of these species based on their genetic relationship.
  • Interestingly, it was found that Przewalskii’s wild horse was within the genetic variation spectrum of the domestic horse. Essentially, the wild and domestic horses were found to be much more closely related than initially assumed.
  • This finding suggests that the change in the chromosome number in Przewalskii’s wild horse (likely increasing) is a relatively recent evolutionary event. The proposed change in chromosome number is another genetic factor besides genetic sequences that influences evolutionary divergence and adaptation.

Cite This Article

APA
Ishida N, Oyunsuren T, Mashima S, Mukoyama H, Saitou N. (1995). Mitochondrial DNA sequences of various species of the genus Equus with special reference to the phylogenetic relationship between Przewalskii’s wild horse and domestic horse. J Mol Evol, 41(2), 180-188. https://doi.org/10.1007/BF00170671

Publication

Journal of molecular evolution
ISSN: 0022-2844
NlmUniqueID: 0360051
Country: Germany
Language: English
Volume: 41
Issue: 2
Pages: 180-188

Researcher Affiliations

Ishida, N
  • Laboratory of Molecular and Cellular Biology, Japan Racing Association, Tokyo.
Oyunsuren, T
    Mashima, S
      Mukoyama, H
        Saitou, N

          MeSH Terms

          • Animals
          • Base Sequence
          • Biological Evolution
          • Chromosomes
          • Conserved Sequence / genetics
          • DNA, Mitochondrial / genetics
          • Genetic Variation / genetics
          • Horses / genetics
          • Molecular Sequence Data
          • Phylogeny
          • RNA, Transfer, Pro / genetics
          • Sequence Alignment
          • Sequence Analysis, DNA

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