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Home / Equine Research Bank / Theriogenology / Mitochondrial oxygen consumption is a unique indicator of st...
Theriogenology2016; 86(5); 1382-1392; doi: 10.1016/j.theriogenology.2016.04.082

Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media.

Abstract: Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin-"LE" and "MFR5", in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 ± 4.8%, P < 0.05). Sperm in EZ Freezin-"LE" had the highest post thaw viability (79 ± 3.1%, P < 0.05) and lowest post thaw ROS production (13 ± 2.4%), but sperm in BotuCrio had the highest maximal oxygen consumption levels, while also demonstrating similar ROS production and viability. This difference would not have been detected using conventional sperm analytical methods. In addition, sperm in BotuCrio had the highest average total motility (49 ± 7.4%), progressive motility (41 ± 6.4%), and velocity (VAP, 90 ± 3.6 μm/s) indicating that this medium preserved mitochondrial function optimally after cryopreservation. Mitochondrial oxygen consumption was positively correlated with traditional measures of sperm function including motility and viability (r = 0.62 and r = 0.49, respectively, P < 0.05), thus making it a sensitive method for determining cryopreservation success and mitochondrial function in stallion sperm.
Copyright © 2016 Elsevier Inc. All rights reserved.
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Publication Date: 2016-05-06 PubMed ID: 27242178DOI: 10.1016/j.theriogenology.2016.04.082Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on how the oxygen consumption rate of horse sperm mitochondria can serve as an accurate measure of their health following cryopreservation, and how this rate varies based on different freezing media used.

Explanation of Terms

  • Mitochondrial oxygen consumption refers to the use of oxygen by mitochondria, the energy-generating organelles within cells, to produce adenosine triphosphate (ATP) – the main source of energy for most cellular processes.
  • Spermatozoa are the mature male sex cells, commonly known as sperm.
  • Cryopreservation is the process of preserving cells or tissues by freezing them to extremely low temperatures.
  • Cryopreservation extenders are solutions used to protect cells during freezing and thawing.
  • Reactive oxygen species (ROS) are chemically reactive chemical species containing oxygen that can cause damage to cell structures.
  • Viability refers to the capability of a cell to survive and function.

Research Methodology

  • The researchers analyzed the oxygen consumption of horse sperm mitochondria when exposed to four different spermatological freezing substances: modified INRA 96, BotuCrio, EZ Freezin-“LE” and “MFR5”.
  • The team evaluated various factors such as motility (ability of the sperm to move efficiently), production of reactive oxygen species (ROS), and viability after freezing and thawing.

Findings

  • All outcomes except for average path rate were significantly influenced by the type of cryopreservation media used.
  • Among all, sperm cryopreserved with INRA 96 showed the lowest average progressive motility once thawed.
  • On the other hand, sperm cryopreserved using EZ Freezin-“LE” demonstrated the highest post-thaw viability and least post-thaw ROS production.
  • However, sperm preserved using BotuCrio had the highest levels of maximum mitochondrial oxygen consumption while having similar ROS production and viability levels.
  • The use of BotuCrio also resulted in the highest average total motility, progressive motility and velocity, hinting at optimal mitochondrial function preservation post-thawing.

Significance of the Study

  • These variations in outcomes would not have been identified using conventional sperm analysis methods.
  • The positive correlation between mitochondrial oxygen consumption, motility, and viability indicates that oxygen consumption serves as a sensitive method to determine the success rate of cryopreservation and mitochondrial function in horse sperm.

Cite This Article

APA
Darr CR, Cortopassi GA, Datta S, Varner DD, Meyers SA. (2016). Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media. Theriogenology, 86(5), 1382-1392. https://doi.org/10.1016/j.theriogenology.2016.04.082

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 86
Issue: 5
Pages: 1382-1392
PII: S0093-691X(16)30125-X

Researcher Affiliations

Darr, Christa R
  • Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, California, USA.
Cortopassi, Gino A
  • Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California, USA.
Datta, Sandipan
  • Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California, USA.
Varner, Dickson D
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA; Veterinary Medical Teaching Hospital, Texas A&M University, College Station, Texas, USA.
Meyers, Stuart A
  • Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, California, USA. Electronic address: smeyers@ucdavis.edu.

MeSH Terms

  • Animals
  • Cryopreservation / veterinary
  • Cryoprotective Agents / pharmacology
  • Horses / physiology
  • Male
  • Mitochondria / physiology
  • Oxygen Consumption / physiology
  • Semen Analysis / veterinary
  • Spermatozoa / physiology

Citations

This article has been cited 21 times.
  1. Bucci D, Spinaci M, Bustamante-Filho IC, Nesci S. The sperm mitochondria: clues and challenges. Anim Reprod 2022;19(4):e20220131.
    doi: 10.1590/1984-3143-AR2022-0131pubmed: 36819482google scholar: lookup
  2. Yin Y, Shen H. Common methods in mitochondrial research (Review). Int J Mol Med 2022 Oct;50(4).
    doi: 10.3892/ijmm.2022.5182pubmed: 36004457google scholar: lookup
  3. Santos JC, Marques CC, Baptista MC, Pimenta J, Teixeira J, Montezinho L, Cagide F, Borges F, Oliveira PJ, Pereira RMLN. Effect of a Novel Hydroxybenzoic Acid Based Mitochondria Directed Antioxidant Molecule on Bovine Sperm Function and Embryo Production. Animals (Basel) 2022 Mar 22;12(7).
    doi: 10.3390/ani12070804pubmed: 35405794google scholar: lookup
  4. Musson R, Gąsior Ł, Bisogno S, Ptak GE. DNA damage in preimplantation embryos and gametes: specification, clinical relevance and repair strategies. Hum Reprod Update 2022 May 2;28(3):376-399.
    doi: 10.1093/humupd/dmab046pubmed: 35021196google scholar: lookup
  5. Orsolini MF, Meyers SA, Dini P. An Update on Semen Physiology, Technologies, and Selection Techniques for the Advancement of In Vitro Equine Embryo Production: Section I. Animals (Basel) 2021 Nov 13;11(11).
    doi: 10.3390/ani11113248pubmed: 34827983google scholar: lookup
  6. Madeja ZE, Podralska M, Nadel A, Pszczola M, Pawlak P, Rozwadowska N. Mitochondria Content and Activity Are Crucial Parameters for Bull Sperm Quality Evaluation. Antioxidants (Basel) 2021 Jul 27;10(8).
    doi: 10.3390/antiox10081204pubmed: 34439451google scholar: lookup
  7. Pintus E, Ros-Santaella JL. Impact of Oxidative Stress on Male Reproduction in Domestic and Wild Animals. Antioxidants (Basel) 2021 Jul 20;10(7).
    doi: 10.3390/antiox10071154pubmed: 34356386google scholar: lookup
  8. Mendoza N, Casao A, Domingo J, Quintín F, Laviña A, Fantova E, Cebrián-Pérez JÁ, Muiño-Blanco T, Pérez-Pe R. Influence of Non-conventional Sperm Quality Parameters on Field Fertility in Ovine. Front Vet Sci 2021;8:650572.
    doi: 10.3389/fvets.2021.650572pubmed: 34026889google scholar: lookup
  9. Gualtieri R, Kalthur G, Barbato V, Di Nardo M, Adiga SK, Talevi R. Mitochondrial Dysfunction and Oxidative Stress Caused by Cryopreservation in Reproductive Cells. Antioxidants (Basel) 2021 Feb 24;10(3).
    doi: 10.3390/antiox10030337pubmed: 33668300google scholar: lookup
  10. Barbagallo F, La Vignera S, Cannarella R, Aversa A, Calogero AE, Condorelli RA. Evaluation of Sperm Mitochondrial Function: A Key Organelle for Sperm Motility. J Clin Med 2020 Jan 29;9(2).
    doi: 10.3390/jcm9020363pubmed: 32013061google scholar: lookup
  11. Marzano G, Moscatelli N, Di Giacomo M, Martino NA, Lacalandra GM, Dell'Aquila ME, Maruccio G, Primiceri E, Chiriacò MS, Zara V, Ferramosca A. Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm. Biology (Basel) 2020 Jan 27;9(2).
    doi: 10.3390/biology9020022pubmed: 32012799google scholar: lookup
  12. Ortiz-Rodriguez JM, Balao da Silva C, Masot J, Redondo E, Gazquez A, Tapia JA, Gil C, Ortega-Ferrusola C, Peña FJ. Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through regulating Akt phosphorylation and reduction of caspase 3. PLoS One 2019;14(7):e0211994.
    doi: 10.1371/journal.pone.0211994pubmed: 31276504google scholar: lookup
  13. Strassner FM, Demattio L, Siuda M, Malama E, Muffels G, Bollwein H. Relationships Between Metabolism of Cryopreserved Equine Sperm Determined by the Seahorse Analyzer and Sperm Characteristics Measured by Flow Cytometry and Computer-Assisted Analysis of Motility. Vet Sci 2025 Nov 21;12(12).
    doi: 10.3390/vetsci12121109pubmed: 41472089google scholar: lookup
  14. Peña FJ, Martín-Cano FE, Becerro-Rey L, da Silva-Álvarez E, Gaitskell-Phillips G, Aparicio IM, Gil MC, Ortega-Ferrusola C. Redox Regulation and Glucose Metabolism in the Stallion Spermatozoa. Antioxidants (Basel) 2025 Feb 17;14(2).
    doi: 10.3390/antiox14020225pubmed: 40002411google scholar: lookup
  15. Anwar K, Thaller G, Saeed-Zidane M. Sperm-Borne Mitochondrial Activity Influenced by Season and Age of Holstein Bulls. Int J Mol Sci 2024 Dec 5;25(23).
    doi: 10.3390/ijms252313064pubmed: 39684774google scholar: lookup
  16. Musatova I, Dzyuba B, Boryshpolets S, Iqbal A, Sotnikov A, Kholodnyy V, Dzyuba V. Evaluation of carp sperm respiration: fluorometry with optochemical oxygen sensor versus polarography. Fish Physiol Biochem 2025 Feb;51(1):16.
    doi: 10.1007/s10695-024-01418-2pubmed: 39630387google scholar: lookup
  17. Bravo A, Sánchez R, Zambrano F, Uribe P. Exogenous Oxidative Stress in Human Spermatozoa Induces Opening of the Mitochondrial Permeability Transition Pore: Effect on Mitochondrial Function, Sperm Motility and Induction of Cell Death. Antioxidants (Basel) 2024 Jun 18;13(6).
    doi: 10.3390/antiox13060739pubmed: 38929178google scholar: lookup
  18. Catalán J, Yánez-Ortiz I, Torres-Garrido M, Ribas-Maynou J, Llavanera M, Barranco I, Yeste M, Miró J. Impact of Seminal Plasma Antioxidants on DNA Fragmentation and Lipid Peroxidation of Frozen-Thawed Horse Sperm. Antioxidants (Basel) 2024 Mar 6;13(3).
    doi: 10.3390/antiox13030322pubmed: 38539855google scholar: lookup
  19. Sushadi PS, Kuwabara M, Maung EEW, Mohamad Mohtar MS, Sakamoto K, Selvaraj V, Asano A. Arresting calcium-regulated sperm metabolic dynamics enables prolonged fertility in poultry liquid semen storage. Sci Rep 2023 Dec 8;13(1):21775.
    doi: 10.1038/s41598-023-48550-2pubmed: 38066036google scholar: lookup
  20. Saratsi A, Samartzi F, Panagiotidis I, Basioura A, Tsiokos D, Ligda C, Rekkas CA. Post-Thaw Parameters of Buck Semen Quality after Soy Lecithin Extender Supplementation with Fumaric Acid. Vet Sci 2023 Sep 12;10(9).
    doi: 10.3390/vetsci10090569pubmed: 37756091google scholar: lookup
  21. Ferreira JJ, Cassina A, Irigoyen P, Ford M, Pietroroia S, Peramsetty N, Radi R, Santi CM, Sapiro R. Increased mitochondrial activity upon CatSper channel activation is required for mouse sperm capacitation. Redox Biol 2021 Dec;48:102176.
    doi: 10.1016/j.redox.2021.102176pubmed: 34753004google scholar: lookup
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