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Theriogenology2016; 86(5); 1382-1392; doi: 10.1016/j.theriogenology.2016.04.082

Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media.

Abstract: Mitochondrial oxygen consumption is a sensitive indicator of spermatozoal health in the context of cryopreservation. We investigated oxygen consumption of equine sperm mitochondria during incubation in four commercially available sperm cryopreservation extenders: modified INRA 96, BotuCrio, EZ Freezin-"LE" and "MFR5", in addition to several other parameters including motility, reactive oxygen species (ROS) production and viability. All experimental endpoints, with the exception of average path velocity, were affected significantly by freezing extender type after freezing and thawing. Sperm in INRA 96 had the lowest average progressive motility after thawing (24 ± 4.8%, P < 0.05). Sperm in EZ Freezin-"LE" had the highest post thaw viability (79 ± 3.1%, P < 0.05) and lowest post thaw ROS production (13 ± 2.4%), but sperm in BotuCrio had the highest maximal oxygen consumption levels, while also demonstrating similar ROS production and viability. This difference would not have been detected using conventional sperm analytical methods. In addition, sperm in BotuCrio had the highest average total motility (49 ± 7.4%), progressive motility (41 ± 6.4%), and velocity (VAP, 90 ± 3.6 μm/s) indicating that this medium preserved mitochondrial function optimally after cryopreservation. Mitochondrial oxygen consumption was positively correlated with traditional measures of sperm function including motility and viability (r = 0.62 and r = 0.49, respectively, P < 0.05), thus making it a sensitive method for determining cryopreservation success and mitochondrial function in stallion sperm.
Copyright © 2016 Elsevier Inc. All rights reserved.
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Publication Date: 2016-05-06 PubMed ID: 27242178DOI: 10.1016/j.theriogenology.2016.04.082Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on how the oxygen consumption rate of horse sperm mitochondria can serve as an accurate measure of their health following cryopreservation, and how this rate varies based on different freezing media used.

Explanation of Terms

  • Mitochondrial oxygen consumption refers to the use of oxygen by mitochondria, the energy-generating organelles within cells, to produce adenosine triphosphate (ATP) – the main source of energy for most cellular processes.
  • Spermatozoa are the mature male sex cells, commonly known as sperm.
  • Cryopreservation is the process of preserving cells or tissues by freezing them to extremely low temperatures.
  • Cryopreservation extenders are solutions used to protect cells during freezing and thawing.
  • Reactive oxygen species (ROS) are chemically reactive chemical species containing oxygen that can cause damage to cell structures.
  • Viability refers to the capability of a cell to survive and function.

Research Methodology

  • The researchers analyzed the oxygen consumption of horse sperm mitochondria when exposed to four different spermatological freezing substances: modified INRA 96, BotuCrio, EZ Freezin-“LE” and “MFR5”.
  • The team evaluated various factors such as motility (ability of the sperm to move efficiently), production of reactive oxygen species (ROS), and viability after freezing and thawing.

Findings

  • All outcomes except for average path rate were significantly influenced by the type of cryopreservation media used.
  • Among all, sperm cryopreserved with INRA 96 showed the lowest average progressive motility once thawed.
  • On the other hand, sperm cryopreserved using EZ Freezin-“LE” demonstrated the highest post-thaw viability and least post-thaw ROS production.
  • However, sperm preserved using BotuCrio had the highest levels of maximum mitochondrial oxygen consumption while having similar ROS production and viability levels.
  • The use of BotuCrio also resulted in the highest average total motility, progressive motility and velocity, hinting at optimal mitochondrial function preservation post-thawing.

Significance of the Study

  • These variations in outcomes would not have been identified using conventional sperm analysis methods.
  • The positive correlation between mitochondrial oxygen consumption, motility, and viability indicates that oxygen consumption serves as a sensitive method to determine the success rate of cryopreservation and mitochondrial function in horse sperm.

Cite This Article

APA
Darr CR, Cortopassi GA, Datta S, Varner DD, Meyers SA. (2016). Mitochondrial oxygen consumption is a unique indicator of stallion spermatozoal health and varies with cryopreservation media. Theriogenology, 86(5), 1382-1392. https://doi.org/10.1016/j.theriogenology.2016.04.082

Publication

Theriogenology
ISSN: 1879-3231
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 86
Issue: 5
Pages: 1382-1392
PII: S0093-691X(16)30125-X

Researcher Affiliations

Darr, Christa R
  • Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, California, USA.
Cortopassi, Gino A
  • Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California, USA.
Datta, Sandipan
  • Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, California, USA.
Varner, Dickson D
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas, USA; Veterinary Medical Teaching Hospital, Texas A&M University, College Station, Texas, USA.
Meyers, Stuart A
  • Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, California, USA. Electronic address: smeyers@ucdavis.edu.

MeSH Terms

  • Animals
  • Cryopreservation / veterinary
  • Cryoprotective Agents / pharmacology
  • Horses / physiology
  • Male
  • Mitochondria / physiology
  • Oxygen Consumption / physiology
  • Semen Analysis / veterinary
  • Spermatozoa / physiology

Citations

This article has been cited 21 times.
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