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European journal of applied physiology2010; 111(3); 549-556; doi: 10.1007/s00421-010-1673-6

Modulation of circulating purines and pyrimidines by physical exercise in the horse.

Abstract: This study was designed to examine the influence of sub-maximal exercise on purine and pyrimidine catabolism in horses. Ten horses were initially trained for 12 weeks at the end of which they underwent a standardized exercise test (SET); venous blood samples were taken at rest, 5 and 30 min after the SET. Six untrained healthy horses, from which a blood withdrawal was taken at rest, were used as the control group. Samples were analyzed by HPLC for the simultaneous determination of uric acid, uridine, β-pseudouridine and creatinine in plasma. Glucose and lactate were measured in blood. Trained horses had basal uridine levels significantly lower than sedentary horses. The SET caused significant increase in plasma uric acid, uridine, β-pseudouridine and creatinine. Following the SET, a significant negative correlation was found between plasma uridine and glucose, whilst a significant positive correlation was observed between plasma uric acid and creatinine. These results indicate that increase in energy demand during exercise in the horse causes not only the degradation of purine but also of pyrimidine compounds, the latter possibly exerting a control on glucose uptake as also demonstrated in human beings.
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Publication Date: 2010-10-08 PubMed ID: 20931219DOI: 10.1007/s00421-010-1673-6Google Scholar: Lookup
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  • Controlled Clinical Trial
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper talks about how sub-maximal physical exercise influences the breakdown of purine and pyrimidine in horses. The study found that increased energy demand during exercise in horses causes not just the degradation of purine but also of pyrimidine compounds, which may have an effect on glucose absorption.

Research Methodology

  • The study involved an experiment on ten horses that were trained for a period of twelve weeks. After their training, they were subjected to a Standardized Exercise Test (SET).
  • At rest, after 5 minutes, and 30 minutes post the SET, samples of venous blood were collected from these trained horses.
  • A control group was established involving six healthy, untrained horses from which blood samples were taken at rest.
  • All the blood samples were analyzed using High-Performance Liquid Chromatography (HPLC) to determine the levels of uric acid, uridine, β-pseudouridine and creatinine in plasma. Additionally, glucose and lactate levels were also measured in the blood.

Key Findings

  • At rest, the trained horses recorded significantly lower basal uridine levels compared to the sedentary or untrained horses.
  • Following the SET, there was significant augmentation in the levels of plasma uric acid, uridine, β-pseudouridine and creatinine.
  • In the aftermath of the SET, a negative correlation was found between plasma uridine and glucose, while a positive correlation was established between plasma uric acid and creatinine.

Implications of the Research

  • The results of the study substantiate that there is an increase in energy demand during exercise in horses, and this leads to not only the degradation of purines but also of pyrimidines.
  • The changes in the levels of these compounds might play a role in the regulation of glucose uptake, similar to findings in human studies.
  • This research could contribute towards understanding the metabolic responses to exercise in horses, and potentially advancing training and performance strategies.

Cite This Article

APA
Alberghina D, Piccione G, Amorini AM, D'Urso S, Longo S, Picardi M, Tavazzi B, Lazzarino G. (2010). Modulation of circulating purines and pyrimidines by physical exercise in the horse. Eur J Appl Physiol, 111(3), 549-556. https://doi.org/10.1007/s00421-010-1673-6

Publication

European journal of applied physiology
ISSN: 1439-6327
NlmUniqueID: 100954790
Country: Germany
Language: English
Volume: 111
Issue: 3
Pages: 549-556

Researcher Affiliations

Alberghina, Daniela
  • Department of Experimental Sciences and Applied Biotechnology, Faculty of Veterinary Medicine, University of Messina, Messina, Italy.
Piccione, Giuseppe
    Amorini, Angela Maria
      D'Urso, Serafina
        Longo, Salvatore
          Picardi, Marika
            Tavazzi, Barbara
              Lazzarino, Giuseppe

                MeSH Terms

                • Animals
                • Chromatography, High Pressure Liquid
                • Creatinine / blood
                • Exercise Test / veterinary
                • Female
                • Horses / blood
                • Horses / physiology
                • Male
                • Motor Activity / physiology
                • Physical Conditioning, Animal / physiology
                • Purines / analysis
                • Purines / blood
                • Pyrimidines / analysis
                • Pyrimidines / blood
                • Uric Acid / analysis
                • Uric Acid / blood

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                Citations

                This article has been cited 2 times.
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                2. Zhang S, Li K, Zhang H, Fu T, Ma Y, Zhang S, Xing G, Tong Y. Progress in animal models for studying hyperuricemia. Front Pharmacol 2025;16:1636205.
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