Molecular Characterization and Serology of Leptospira kirschneri (Serogroup Grippotyphosa) Isolated from Urine of a Mare Post-Abortion in Brazil.
Abstract: A strain of Leptospira kirschneri (serogroup Grippotyphosa) was cultured from urine of a mare post-abortion in Brazil and characterized by serogrouping, multiple-locus variable-number tandem repeat analysis, PGFE, and sequencing of genes rrs and secY. Strains of L. kirschneri have apparently never been recovered from horses in tropical area, only in Europe and USA. Knowledge of local epidemiology is important to interpret genetic profiles of leptospires circulating in an area.
© 2015 Blackwell Verlag GmbH.
Publication Date: 2015-09-10 PubMed ID: 26355500DOI: 10.1111/zph.12224Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article primarily deals with the identification and analysis of a strain of the bacterium Leptospira kirschneri that was found in a mare’s urine after an abortion in Brazil. The specific strain was unusual because such types of bacteria have never been reported in horses in tropical climates before.
Leptospira Kirschneri Strain Identification
- The study paves a new path in the investigation of Leptospira strains as it successfully cultured a new strain of Leptospira kirschneri from the urine of a mare in Brazil that had recently undergone an abortion.
- The strain belongs to the serogroup Grippotyphosa; a classification of the bacterium based on its antigenic properties.
Research Methods Utilised
- Strategic scientific methods and techniques were employed to conduct the study. The study uses serogrouping, a method of classification based on antigenic determinants present on the bacterium for characterizing the isolated strain.
- Multiple-locus variable-number tandem repeat analysis (MLVA) was used. This molecular biology technique allows the identification and typing of bacterial strains based on the number of repetitions of specific DNA sequences.
- Pulsed-Field Gel Electrophoresis (PFGE) was used, a technique allowing the separation and comparison of large DNA molecules by applying electrical pulses.
- The SecY gene, a gene encoding a protein involved in protein secretion, and the rrs gene, a gene coding for a component of the bacterial ribosome were sequenced to examine their genetic structures.
Significance of the Findings
- The importance of this research lies in the fact that this strain of Leptospira kirschneri had never been recovered from horses from tropical regions (like Brazil). Previous findings were limited to instances in Europe and the USA. This discovery can enable better understanding of the geographical spread and diversity of the bacterium.
- Understanding the bacterial strains circulating in a particular geographical area contributes significantly to local epidemiology. This knowledge can help in designing effective prevention and control strategies and treat infections more accurately.
Cite This Article
APA
Hamond C, Martins G, Bremont S, Medeiros MA, Bourhy P, Lilenbaum W.
(2015).
Molecular Characterization and Serology of Leptospira kirschneri (Serogroup Grippotyphosa) Isolated from Urine of a Mare Post-Abortion in Brazil.
Zoonoses Public Health, 63(3), 191-195.
https://doi.org/10.1111/zph.12224 Publication
Researcher Affiliations
- Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
- Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
- Unité de Biologie des Spirochètes, Institut Pasteur, National Reference Center, Paris, France.
- Bio-Manguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Rio de Janeiro, RJ, Brazil.
- Unité de Biologie des Spirochètes, Institut Pasteur, National Reference Center, Paris, France.
- Laboratory of Veterinary Bacteriology, Department of Microbiology and Parasitology, Universidade Federal Fluminense, Niterói, RJ, Brazil.
MeSH Terms
- Abortion, Veterinary
- Animals
- Bacterial Shedding
- Bacterial Typing Techniques
- Brazil / epidemiology
- Female
- Horse Diseases / epidemiology
- Horse Diseases / microbiology
- Horses
- Leptospira / classification
- Leptospira / genetics
- Leptospira / isolation & purification
- Leptospirosis / epidemiology
- Leptospirosis / microbiology
- Leptospirosis / veterinary
- Multilocus Sequence Typing
- Phylogeny
- Pregnancy
- Public Health
- Sequence Analysis, DNA / veterinary
- Serogroup
- Urine / microbiology
- Zoonoses
Citations
This article has been cited 7 times.- de Andrade Morais D, Nunes BC, Soares RR, de Oliveira MD, da Costa DF, de Araújo HG, Júnior JPA, Malossi CD, Silva MLCR, de Azevedo SS, Alves CJ. Strong Evidence of the Role of Donkeys in the Epidemiology of Leptospira spp. in Semiarid Conditions.. Microorganisms 2023 Jul 22;11(7).
- Harran E, Pinot A, Kodjo A, Djelouadji Z, Le Gudayer M, Sionfoungo Daouda S, Groud K, Lattard V, Ayral F. Identification of Pathogenic Leptospira kirschneri Serogroup Grippotyphosa in Water Voles (Arvicola terrestris) from Ruminant Pastures in Puy-de-Dôme, Central France.. Pathogens 2023 Feb 6;12(2).
- Schmidt E, Obiegala A, Imholt C, Drewes S, Saathoff M, Freise J, Runge M, Jacob J, Mayer-Scholl A, Ulrich RG, Pfeffer M. Influence of Season, Population and Individual Characteristics on the Prevalence of Leptospira spp. in Bank Voles in North-West Germany.. Biology (Basel) 2021 Sep 18;10(9).
- Benitez ADN, Monica TC, Miura AC, Romanelli MS, Giordano LGP, Freire RL, Mitsuka-Breganó R, Martins CM, Biondo AW, Serrano IM, Lopes THCR, Reis RB, Gomes JF, Costa F, Wunder E, Ko AI, Navarro IT. Spatial and Simultaneous Seroprevalence of Anti-Leptospira Antibodies in Owners and Their Domiciled Dogs in a Major City of Southern Brazil.. Front Vet Sci 2020;7:580400.
- Murcia CA, Astudillo M, Romero MH. Prevalence of leptospirosis in vaccinated working dogs and humans with occupational risk.. Biomedica 2020 May 1;40(Supl. 1):62-75.
- Alvarado-Esquivel C, Cruz-Romero A, Romero-Salas D, Alvarado-Félix ÁO, Aguilar-Domínguez M, Ochoa-Valencia JL, Alvarado-Esquivel D, Hernández-Tinoco J, Zamarripa-Barboza JA, Sánchez-Anguiano LF. Apparently high Leptospira antibody seropositivity in donkeys for slaughter in three municipalities in Durango, Mexico.. J Vet Diagn Invest 2018 Nov;30(6):929-932.
- Libonati HA, Santos GB, Souza GN, Brandão FZ, Lilenbaum W. Leptospirosis is strongly associated to estrus repetition on cattle.. Trop Anim Health Prod 2018 Oct;50(7):1625-1629.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
