Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.
Abstract: A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could effectively differentiate B. caballi-infected horse sera from Babesia equi-infected or noninfected control horse sera. These results suggest that the recombinant BC134 protein is a potential diagnostic antigen in the detection of B. caballi infection.
Publication Date: 2004-01-13 PubMed ID: 14715570PubMed Central: PMC321337DOI: 10.1128/cdli.11.1.211-215.2004Google Scholar: Lookup
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Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The paper relates to the cloning of a specific gene in Babesia caballi, a parasite that infects horses. The gene produces a specific protein that can be used in a diagnostic test to identify cases of B. caballi infection.
Method
- The researchers began by identifying and cloning a gene in Babesia caballi, a unicellular parasite that infects horses and other equids.
- This specific gene codes for a protein weighing 134 kilodaltons, referred to as BC134.
- The presence of this protein in the blood of horses was identified using an immunoassay, a laboratory method that measures the concentration of a substance in a solution.
- They used a type of immunoassay called an enzyme-linked immunosorbent assay (ELISA), which is a common diagnostic tool in medicine and pathology.
Results
- During the screening process, the researchers tested the BC134 protein against horse serum infected with B. caballi.
- They found that an ELISA using the BC134 protein could accurately distinguish between horse sera infected with B. caballi and those infected with Babesia equi, another horse parasite, or non-infected control sera.
Potential Applications
- This indicates that the BC134 protein could be used as a diagnostic marker in the detection of B. caballi infection, which currently lacks reliable testing methods.
- This has potential implications for the management of B. caballi in horse populations, improving the ability of veterinarians and researchers to identify and treat this infection early and effectively.
- The findings of this study may also contribute to wider knowledge of the biology of B. caballi and other similar parasites, informing future research and treatment approaches.
Cite This Article
APA
Tamaki Y, Hirata H, Takabatake N, Bork S, Yokoyama N, Xuan X, Fujisaki K, Igarashi I.
(2004).
Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.
Clin Diagn Lab Immunol, 11(1), 211-215.
https://doi.org/10.1128/cdli.11.1.211-215.2004 Publication
Researcher Affiliations
- National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
MeSH Terms
- Amino Acid Sequence
- Animals
- Antigens, Protozoan / chemistry
- Antigens, Protozoan / genetics
- Babesia / genetics
- Babesia / immunology
- Babesiosis / diagnosis
- Babesiosis / immunology
- Babesiosis / veterinary
- Base Sequence
- Cloning, Molecular
- DNA, Protozoan / genetics
- Enzyme-Linked Immunosorbent Assay / methods
- Genes, Protozoan
- Horse Diseases / diagnosis
- Horse Diseases / immunology
- Horses
- Molecular Sequence Data
- Molecular Weight
- Protozoan Proteins / chemistry
- Protozoan Proteins / genetics
- Protozoan Proteins / immunology
- Recombinant Proteins / chemistry
- Recombinant Proteins / genetics
- Recombinant Proteins / immunology
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Citations
This article has been cited 2 times.- El-Sayed SAE, Rizk MA, Baghdadi HB, Ringo AE, Sambuu G, Nugraha AB, Igarashi I. Development of a promising antigenic cocktail for the global detection of Babesia caballi in horse by ELISA.. PLoS One 2023;18(4):e0284535.
- Hirata H, Yokoyama N, Xuan X, Fujisaki K, Suzuki N, Igarashi I. Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis.. Clin Diagn Lab Immunol 2005 Feb;12(2):334-8.
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