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Veterinary sciences2022; 9(4); 187; doi: 10.3390/vetsci9040187

Molecular Detection of Equine Adenovirus 1 in Nasal Swabs from Horses in the Republic of Korea.

Abstract: Equine adenovirus 1 (EAdV-1) can cause upper respiratory disease in horses and has been reported worldwide. In this study, and for the first time in Korea, the prevalence of EAdV-1 in equine nasal swabs was investigated using a PCR to identify potential risk factors and examine the genetic diversity of its DNA sequences by a comparison with foreign strains. Nasal swabs collected from 359 horses reared at Korea Racing Authority facilities were tested using an EAdV-1 hexon-specific PCR and the associations between EAdV-1 infection and sex, age, region, breed, and activity were analyzed. Five samples (1.4%, 5/359) tested positive for EAdV-1; however, no statistically significant differences were observed with respect to any variable. Among the five EAdV-1-positive horses, a co-infection with equine influenza, equine herpesvirus 1 and 4, or was not detected; however, clinical respiratory signs were observed in one. Phylogenetic analyses based on partial EAdV-1 hexon gene sequences revealed that the Korean EAdV-1 isolates shared approximately 98.8-100% similarity among each other and with foreign strains. Three Korean isolates shared high similarity with strains from Australia and India and the remaining two isolates were separate in phylogenetic analyses. These findings highlight the molecular prevalence and genetic diversity of EAdV-1 in horses in Korea.
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Publication Date: 2022-04-13 PubMed ID: 35448685PubMed Central: PMC9025330DOI: 10.3390/vetsci9040187Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper outlines a study conducted on the prevalence and genetic diversity of the Equine adenovirus 1 (EAdV-1) in horses in Korea. This is the first time such a study was conducted in the country.

Overview and Motive

  • The study was motivated by the need to understand the prevalence and diversity of the Equine adenovirus 1 (EAdV-1), a virus known to cause respiratory disease in horses, in Korea.
  • This pathogen had not been investigated in this region beforehand, thus the paper explores its existence in domestic horse populations through the careful collection and analysis of nasal swab samples.
  • The goal was to identify potential risk factors and understand the genetic diversity of EAdV-1 by comparing its DNA with foreign pathogens.

Methodology

  • Researchers collected nasal swabs from a total of 359 horses, all of which were reared in facilities of the Korea Racing Authority.
  • A PCR (polymerase chain reaction) test targeting EAdV-1’s hexon gene was used for the detection. This gene is crucial as it codes for one of the main components of the virus’s outer layer and is commonly used in such studies because of its high mutation rate.
  • Associations between EAdV-1 infection and various factors including sex, age, breed, region, and activity level were taken into consideration.

Findings

  • Out of the total samples, only 1.4% (5 horses) tested positive for EAdV-1.
  • The data showed no significant statistical difference when the virus prevalence was compared against the variables of sex, age, region, breed, and activity.
  • None of the five affected horses exhibited co-infection with other equine diseases such as equine influenza and equine herpesvirus 1 and 4; however, respiratory signs were observed in one case.

Genetic Diversity based on Phylogenetic Analysis

  • The researchers analyzed the genetic diversity of EAdV-1 in Korea by sequencing the hexon gene of collected samples, comparing it with international strains.
  • It was found that the Korean EAdV-1 sequences showed a high similarity, approximately 98.8-100%, among each other and with foreign strains.
  • Three Korean strains were very similar to the strains found in Australia and India while the remaining two were distinct.

Conclusion

  • This research has successfully provided estimates on the prevalence and genetic diversity of EAdV-1 within Korean horse populations.
  • The insights gained can be utilized for future research and establishment of mitigating strategies to combat EAdV-1.

Cite This Article

APA
Lee SK, Choi J, Yoon J, Jung J, Park JY, Park J, Kim Y, Park JY, Park D. (2022). Molecular Detection of Equine Adenovirus 1 in Nasal Swabs from Horses in the Republic of Korea. Vet Sci, 9(4), 187. https://doi.org/10.3390/vetsci9040187

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 9
Issue: 4
PII: 187

Researcher Affiliations

Lee, Sang-Kyu
  • Veterinary Center, Korea Racing Authority, Gwacheon 13822, Korea.
Choi, Jeechan
  • Busan Equine Hospital, Korea Racing Authority, Busan 46745, Korea.
Yoon, Jungho
  • Equine Clinic, Jeju Regional Headquarter, Korea Racing Authority, Jeju 63346, Korea.
Jung, Jaemin
  • Equine Clinic, Jeju Regional Headquarter, Korea Racing Authority, Jeju 63346, Korea.
Park, Joon-Young
  • Jangsu Equine Hospital, Korea Racing Authority, Jangsu 55620, Korea.
Park, Jongyoung
  • Equine Clinic, Jeju Regional Headquarter, Korea Racing Authority, Jeju 63346, Korea.
Kim, Yeonjong
  • Veterinary Center, Korea Racing Authority, Gwacheon 13822, Korea.
Park, Ji-Young
  • Animal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, Korea.
Park, Dongsun
  • Department of Biology Education, Korea National University of Education, Cheongju 28173, Korea.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 36 references
  1. Gilkerson JR, Bailey KE, Diaz-Méndez A, Hartley CA. Update on viral diseases of the equine respiratory tract.. Vet Clin North Am Equine Pract 2015 Apr;31(1):91-104.
    doi: 10.1016/j.cveq.2014.11.007pubmed: 25648568google scholar: lookup
  2. Powell DG, Burrows R, Goodridge D. Respiratory viral infections among thoroughbred horses in training during 1972.. Equine Vet J 1974 Jan;6(1):19-24.
    doi: 10.1111/j.2042-3306.1974.tb03922.xpubmed: 4362234google scholar: lookup
  3. Morley P.S., Pusterla N.. Viral respiratory disease in athletic horses. Equine Sports Medicine and Surgery 2nd ed. W.B. Saunders; St. Louis, MO, USA: 2014. pp. 649–664.
  4. Traub-Dargatz JL, Salman MD, Voss JL. Medical problems of adult horses, as ranked by equine practitioners.. J Am Vet Med Assoc 1991 May 15;198(10):1745-7.
    pubmed: 2071472
  5. MacLachlan N.J., Dubovi E.J.. Adenoviridae. Fenner’s Veterinary Virology 5th ed. Academic Press; Boston, MA, USA: 2017. pp. 217–227.
  6. Giles C, Cavanagh HM, Noble G, Vanniasinkam T. Prevalence of equine adenovirus antibodies in horses in New South Wales, Australia.. Vet Microbiol 2010 Jul 14;143(2-4):401-4.
    doi: 10.1016/j.vetmic.2009.11.042pubmed: 20060662google scholar: lookup
  7. McKenzie H.C.. Disorders of Foals. Equine Internal Medicine 4th ed. W.B. Saunders; St. Louis, MO, USA: 2018. p. 1393.
  8. Savage C.J., Middleton D., Studdert M.J.. Adeno, Hendra, and Equine Rhinitis Viral Respiratory Diseases. Equine Infectious Diseases 2nd ed. W.B. Saunders; St. Louis, MO, USA: 2014. pp. 189–197.
  9. Studdert MJ, Wilks CR, Coggins L. Antigenic comparisons and serologic survey of equine adenoviruses.. Am J Vet Res 1974 May;35(5):693-9.
    pubmed: 4364459
  10. Reubel GH, Studdert MJ. Identification, cloning and sequence analysis of the equine adenovirus 1 hexon gene.. Arch Virol 1997;142(6):1193-212.
    doi: 10.1007/s007050050152pubmed: 9229008google scholar: lookup
  11. Love S., Mair T.S.. Infectious diseases and parasitology. Equine Medicine, Surgery and Reproduction 2nd ed. W.B. Saunders; Oxford, UK: 2012. p. 404.
  12. Cavanagh HM, Mahony TJ, Vanniasinkam T. Genetic characterization of equine adenovirus type 1.. Vet Microbiol 2012 Feb 24;155(1):33-7.
    doi: 10.1016/j.vetmic.2011.08.014pubmed: 21924564google scholar: lookup
  13. DARBYSHIRE JH, PEREIRA HG. AN ADENOVIRUS PRECIPITATING ANTIBODY PRESENT IN SOME SERA OF DIFFERENT ANIMAL SPECIES AND ITS ASSOCIATION WITH BOVINE RESPIRATORY DISEASE.. Nature 1964 Feb 29;201:895-7.
    doi: 10.1038/201895a0pubmed: 14132783google scholar: lookup
  14. Kamada M.. Comparison of the Four Serological Tests for Detecting Antibodies against Equine Adenovirus. Bull. Equine Res. Inst. 1978;1978:91–96.
  15. Kamada M., Akiyama Y.. A Survey on Precipitating Antibody against Adenovirus in Light Horses of Japan. Bull. Equine Res. Inst. 1977;1977:29–37.
  16. Harden TJ, Pascoe RR, Spradbrow PB, Johnston KG. The prevalence of antibodies to adenoviruses in horses from queensland and New South Wales.. Aust Vet J 1974 Nov;50(11):477-82.
    doi: 10.1111/j.1751-0813.1974.tb14051.xpubmed: 4374922google scholar: lookup
  17. Horner GW, Hunter R. Isolation of two serotypes of equine adenovirus from horses in New Zealand.. N Z Vet J 1982 May;30(5):62-4.
    doi: 10.1080/00480169.1982.34882pubmed: 16030868google scholar: lookup
  18. Dunowska M, Wilks CR, Studdert MJ, Meers J. Equine respiratory viruses in foals in New Zealand.. N Z Vet J 2002 Aug;50(4):140-7.
    doi: 10.1080/00480169.2002.36300pubmed: 16032260google scholar: lookup
  19. Timoney PJ. Adenovirus precipitating antibodies in the sera of some domestic animal species in Ireland.. Br Vet J 1971 Dec;127(12):567-71.
    doi: 10.1016/S0007-1935(17)37233-0pubmed: 4335898google scholar: lookup
  20. Obi TU, Taylor WP. Serological survey of adenovirus antibodies in domestic animals in Nigeria.. Comp Immunol Microbiol Infect Dis 1984;7(1):63-8.
    doi: 10.1016/0147-9571(84)90017-1pubmed: 6086225google scholar: lookup
  21. de Boer GF, Osterhaus AD, van Oirschot JT, Wemmenhove R. Prevalence of antibodies to equine viruses in the Netherlands.. Vet Q 1979 Apr;1(2):65-74.
    doi: 10.1080/01652176.1979.9693724pubmed: 22039753google scholar: lookup
  22. Ataseven VS, Oğuzoğlu TÇ, Başaran-Karapınar Z, Bilge-Dağalp S. First genetic characterization of equine adenovirus type 1 (EAdV-1) in Turkey.. Res Vet Sci 2012 Apr;92(2):324-6.
    doi: 10.1016/j.rvsc.2011.03.001pubmed: 21440276google scholar: lookup
  23. Dynon K, Varrasso A, Ficorilli N, Holloway S, Reubel G, Li F, Hartley C, Studdert M, Drummer H. Identification of equine herpesvirus 3 (equine coital exanthema virus), equine gammaherpesviruses 2 and 5, equine adenoviruses 1 and 2, equine arteritis virus and equine rhinitis A virus by polymerase chain reaction.. Aust Vet J 2001 Oct;79(10):695-702.
    doi: 10.1111/j.1751-0813.2001.tb10674.xpubmed: 11712710google scholar: lookup
  24. Bell SA, Leclere M, Gardner IA, Maclachlan NJ. Equine adenovirus 1 infection of hospitalised and healthy foals and horses.. Equine Vet J 2006 Jul;38(4):379-81.
    doi: 10.2746/042516406777749173pubmed: 16866210google scholar: lookup
  25. Doubli-Bounoua N, Richard EA, Léon A, Pitel PH, Pronost S, Fortier G. Multiple molecular detection of respiratory viruses and associated signs of airway inflammation in racehorses.. Virol J 2016 Nov 29;13(1):197.
    doi: 10.1186/s12985-016-0657-5pmc: PMC5129218pubmed: 27899161google scholar: lookup
  26. Broux B., Gryspeerdt A., Amory H., Frippiat T., Gasthuys F., Legrand L., Deprez P.. Prevalence of respiratory pathogens in nasal swabs from horses with acute respiratory disease in Belgium. Vlaams Diergeneeskd. Tijdschr. 2016;85:221–224.
    doi: 10.21825/vdt.v85i4.16332google scholar: lookup
  27. Wilks CR, Studdert MJ. Isolation of an equine adenovirus.. Aust Vet J 1972 Oct;48(10):580-1.
    doi: 10.1111/j.1751-0813.1972.tb08031.xpubmed: 4649722google scholar: lookup
  28. Ryu SH, Koo HC, Park YK, Kim JM, Jung WK, Davis WC, Park YH, Lee CW. Etiologic and immunologic characteristics of thoroughbred horses with bacterial infectious upper respiratory disease at the Seoul Race Park.. J Microbiol Biotechnol 2009 Sep;19(9):1041-50.
    doi: 10.4014/jmb.0812.670pubmed: 19809264google scholar: lookup
  29. Ko S., Kang J.-G., Yeh J.-Y., Moon J.-S., Choi G.-C., Won S., Chae J.-S.. First Report on Molecular Detection of Equine Upper Respiratory Infectious Viruses in Republic of Korea. J. Equine Vet. Sci. 2013;33:628–636.
    doi: 10.1016/j.jevs.2012.11.001google scholar: lookup
  30. Tamura K, Stecher G, Kumar S. MEGA11: Molecular Evolutionary Genetics Analysis Version 11.. Mol Biol Evol 2021 Jun 25;38(7):3022-3027.
    doi: 10.1093/molbev/msab120pmc: PMC8233496pubmed: 33892491google scholar: lookup
  31. Burrows R., Goodridge D.. Observations of Picornavirus, Adenovirus, and Equine Herpesvirus Infections in the Pirbright Pony Herd. Proceedings of the Fourth International Conference on Equine Infectious Diseases Lyon, France. 24–27 September 1976; Princeton, NJ, USA: Veterinary Publications Inc.; 1978. pp. 155–164.
  32. McChesney A.E., England J.J.. Equine adenoviral infection: Pathogenesis of experimentally and naturally transmitted infection. Proceedings of the Fourth International Conference on Equine Infectious Diseases Lyon, France. 24–27 September 1976; Princeton, NJ, USA: Veterinary Publications Inc.; 1976. pp. 141–145.
  33. Korean Statistical Information Service Horse Industry Survey. [(accessed on 1 March 2022)]; Available online: https://kosis.kr/statHtml/statHtml.do?orgId=114&tblId=DT_114051N_003&conn_path=I2.
  34. Korean Statistical Information Service Cadastral Statistics. [(accessed on 1 March 2022)]; Available online: https://kosis.kr/statHtml/statHtml.do?orgId=116&tblId=DT_MLTM_1246&conn_path=I2.
  35. Powell DG. Viral respiratory disease of the horse.. Vet Clin North Am Equine Pract 1991 Apr;7(1):27-52.
    doi: 10.1016/S0749-0739(17)30514-Xpubmed: 1647261google scholar: lookup
  36. Pusterla N, Kass PH, Mapes S, Johnson C, Barnett DC, Vaala W, Gutierrez C, McDaniel R, Whitehead B, Manning J. Surveillance programme for important equine infectious respiratory pathogens in the USA.. Vet Rec 2011 Jul 2;169(1):12.
    doi: 10.1136/vr.d2157pubmed: 21676986google scholar: lookup

Citations

This article has been cited 1 times.
  1. Enkhbat M, Batzorig U, Dashdondog N, Trujillo-Vargas CM, Dambadarjaa D, Gray GC. The Highly Interrelated Morbidity Respiratory Viruses Cause Among Humans and Animals in Mongolia. Viruses 2025 Nov 28;17(12).
    doi: 10.3390/v17121557pubmed: 41472228google scholar: lookup
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