Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland.
Abstract: Clinical isolates (n = 63) of Pseudomonas aeruginosa obtained from various sites in 63 horses were compared using ERIC2 RAPD PCR to determine their genetic relatedness. Resulting banding patterns (n = 24 genotypes) showed a high degree of genetic heterogeneity amongst all isolates examined, indicating a relative non-clonal relationship between isolates from these patients, employing this genotyping technique. This study characterised 63 clinical isolates into 24 distinct genotypes, with the largest cluster (genotype E) accounting for 10/63 (15.9%) of the isolates. ERIC2 RAPD PCR proved to be a highly discriminatory molecular typing tool of P. aeruginosa in isolates recovered from horses. With the adoption of several controls to aid reproducibility, this technique may be useful as an alternative to PFGE, particularly in epidemiological investigations of outbreaks where speed may be a significant parameter. This is the first report of clonal heterogeneity amongst P. aeruginosa from horses and demonstrated that ERIC RAPD PCR is a rapid method for the examination of this species in horses, which may be useful in outbreak analysis.
Publication Date: 2009-07-01 PubMed ID: 21851737PubMed Central: PMC3113754DOI: 10.1186/2046-0481-62-7-456Google Scholar: Lookup
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- Journal Article
Summary
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The research investigates the genetic relationships among clinical isolates of Pseudomonas aeruginosa bacteria from horses in Ireland, finding a high degree of genetic diversity using ERIC2 RAPD PCR as a typing tool.
Objectives and Methodology
- The study looked at 63 clinical isolates of Pseudomonas aeruginosa, a bacteria, from various sites in 63 different horses.
- The researchers used a method called ERIC2 RAPD PCR to compare these isolates and assess their genetic relatedness.
- This technique is a molecular typing tool that scientists use to classify different strains of bacteria based on their genetic DNA profiles.
Findings
- The results from the ERIC2 RAPD PCR method showed a high degree of genetic diversity (heterogeneity) between all of the isolates studied.
- Among the 63 clinical isolates, the researchers identified 24 distinct genotypes, demonstrating that the isolates were not clonally related, that is, they were not identical or very closely related genetically.
- The most prominent cluster, referred to as genotype E in the study, included 10 out of the 63 isolates, or about 15.9% of the total.
Implications and Conclusions
- The study proved the high discriminative power of the ERIC2 RAPD PCR method for typing P. aeruginosa in isolates deriving from horses.
- The technique could therefore be a useful tool in epidemiological investigations into outbreaks of infections caused by this bacterium, thanks to its speed. This may be a valuable alternative to Pulsed Field Gel Electrophoresis (PFGE), another common yet more time-intensive method of bacterial typing.
- This research is the first to report on the clonal diversity of P. aeruginosa from horses, highlighting the complexity of this bacteria’s population in equines.
- The rapidity and efficiency of ERIC RAPD PCR may also prove useful in managing outbreaks in horse populations, by providing timely and accurate typing information.
Cite This Article
APA
Tazumi A, Maeda Y, Buckley T, Millar B, Goldsmith C, Dooley J, Elborn J, Matsuda M, Moore J.
(2009).
Molecular epidemiology of clinical isolates of Pseudomonas aeruginosa isolated from horses in Ireland.
Ir Vet J, 62(7), 456-459.
https://doi.org/10.1186/2046-0481-62-7-456 Publication
Researcher Affiliations
- Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland. jemoore@niphl.dnet.co.uk.
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This article includes 11 references
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Citations
This article has been cited 4 times.- Hassuna NA, Mandour SA, Mohamed ES. Virulence Constitution of Multi-Drug-Resistant Pseudomonas aeruginosa in Upper Egypt. Infect Drug Resist 2020;13:587-595.
- Han MM, Mu LZ, Liu XP, Zhao J, Liu XF, Liu H. ERIC-PCR genotyping of Pseudomonas aeruginosa isolates from haemorrhagic pneumonia cases in mink. Vet Rec Open 2014;1(1):e000043.
- Scholtz M, Guthrie AJ, Newton R, Schulman ML. Review of Pseudomonas aeruginosa and Klebsiella pneumoniae as venereal pathogens in horses. Equine Vet J 2025 May;57(3):587-597.
- Lupo A, Haenni M, Madec JY. Antimicrobial Resistance in Acinetobacter spp. and Pseudomonas spp. Microbiol Spectr 2018 Jun;6(3).
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