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Parasitology research2022; 122(1); 207-215; doi: 10.1007/s00436-022-07719-w

Molecular identification of Trypanosoma cruzi in domestic animals in municipalities of the State of Rio Grande do Norte, Brazil.

Abstract: Trypanosoma cruzi, the etiologic agent of American trypanosomiasis, is a vector-borne zoonotic parasite which has been little studied regarding its infection in domestic animals. In this study, we evaluated the occurrence of natural infection by T. cruzi in farm animals using molecular markers and phylogenetic analysis in blood clot samples of 60 sheep (Ovis aires), 22 goats (Capra hircus), and 14 horses (Equus caballus) in eight municipalities located in an infection risk area in the state of Rio Grande do Norte (RN), Northeast Region of Brazil. Trypanosoma spp. infection was identified by amplifying the rRNA 18S SSU gene in 48.9% of the samples. The SH022 sample showed 99.8% similarity with the Y strain of T. cruzi in phylogeny, grouped in the DTU II clade. Blood clots of sheep, goats, and horses detected T. cruzi kDNA in 28.3% (17/60), 22.7% (5/22), and 15.4% (2/14) of the samples, respectively. These animals were distributed in the three studied mesoregions throughout the state of RN. The identification of natural infection in domestic animals contributes to expand the epidemiological transmission scenario in an area where T. brasiliensis is the main vector.
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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Publication Date: 2022-11-21 PubMed ID: 36404367DOI: 10.1007/s00436-022-07719-wGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied the occurrence of a parasite, Trypanosoma cruzi, in domestic animals in the state of Rio Grande do Norte in Brazil. The parasite was found in sheep, goats, and horses often enough to indicate it may have a broader impact on animal health in the region than previously assumed.

Objective and Methodology of the Study

  • The researchers aimed to understand the occurrence of the Trypanosoma cruzi parasite, which causes American trypanosomiasis disease, in domestic animals in Brazil.
  • They collected blood samples from 60 sheep, 22 goats, and 14 horses in eight municipalities in the risk area of the state of Rio Grande do Norte.
  • The team used molecular markers and phylogenetic analysis to identify the parasite. They amplified the rRNA 18S SSU gene to detect Trypanosoma spp. infection.

Key Findings of the Study

  • Close to half, 48.9% of the evaluated samples were found to be infected with Trypanosoma spp.
  • One of the samples (SH022) showed a huge similarity (99.8%) with the Y strain of T. cruzi, indicating a possibility of same strain of the parasite occurring in different species.
  • Analysis of blood clots from the various animals showed T. cruzi kDNA in 28.3% of sheep, 22.7% of goats and 15.4% of horses.
  • The infected animals were distributed across all three studied mesoregions of the state, suggesting a wide spread of the infection.

Implications of the Research and Final Thoughts

  • This study shows that the T. cruzi infection is common in domestic animals in the state of Rio Grande do Norte, Brazil, with potential implications for public health and the local economy.
  • The presence of the parasite in three different species of animals shows that it has a wide host range. This suggests the potential for transmission to other animals, and possibly humans, which poses a public health concern.
  • This research contributes to the understanding of the transmission dynamics of T. cruzi, supporting future parasite control and prevention strategies in domestic animals.
  • Further research is needed to assess the risk of transmission to humans and other animals and to develop strategies for limiting the spread and impact of this parasite.

Cite This Article

APA
de Araújo-Neto VT, Barbosa-Silva AN, Medeiros Honorato NR, Sales LML, de Cassia Pires R, do Nascimento Brito CR, da Matta Guedes PM, da Cunha Galvão LM, da Câmara ACJ. (2022). Molecular identification of Trypanosoma cruzi in domestic animals in municipalities of the State of Rio Grande do Norte, Brazil. Parasitol Res, 122(1), 207-215. https://doi.org/10.1007/s00436-022-07719-w

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 122
Issue: 1
Pages: 207-215

Researcher Affiliations

de Araújo-Neto, Vicente Toscano
  • Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Norte, Natal, 59012-570, Brazil.
Barbosa-Silva, Andressa Noronha
  • Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Norte, Natal, 59012-570, Brazil.
Medeiros Honorato, Nathan Ravi
  • Graduate Program in Parasitology, Federal University of Minas Gerais, Belo Horizonte, Belo Horizonte, 31270-901, Brazil.
Sales, Letícia Mikardya Lima
  • Undergraduate Course in Pharmacy, Federal University of Rio Grande Do Norte, Natal, 59012-570, Brazil.
de Cassia Pires, Renata
  • Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Norte, Natal, 59012-570, Brazil.
do Nascimento Brito, Carlos Ramon
  • Graduate Program in Parasite Biology, Federal University of Rio Grande Do Norte, Natal, 59064-741, Brazil.
da Matta Guedes, Paulo Marcos
  • Graduate Program in Parasite Biology, Federal University of Rio Grande Do Norte, Natal, 59064-741, Brazil.
da Cunha Galvão, Lúcia Maria
  • Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Norte, Natal, 59012-570, Brazil.
  • Graduate Program in Parasitology, Federal University of Minas Gerais, Belo Horizonte, Belo Horizonte, 31270-901, Brazil.
da Câmara, Antonia Claudia Jácome
  • Graduate Program in Pharmaceutical Sciences, Federal University of Rio Grande Do Norte, Natal, 59012-570, Brazil. acjcamara@ufrnet.br.
  • Graduate Program in Parasite Biology, Federal University of Rio Grande Do Norte, Natal, 59064-741, Brazil. acjcamara@ufrnet.br.

MeSH Terms

  • Animals
  • Sheep
  • Trypanosoma cruzi / genetics
  • Animals, Domestic / parasitology
  • Brazil / epidemiology
  • Phylogeny
  • Cities
  • Insect Vectors / parasitology
  • Chagas Disease / epidemiology
  • Chagas Disease / veterinary
  • Chagas Disease / parasitology
  • Goats
  • Triatoma / genetics

Grant Funding

  • granting scholarships / Coordenau00e7u00e3o de Aperfeiu00e7oamento de Pessoal de Nu00edvel Superior (CAPES)
  • MCTI/CNPq/Edital Universal 2016 grant no.423966/2016-2 / Conselho Nacional de Desenvolvimento Cientu00edfico e Tecnolu00f3gico

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