Monitoring of effects induced by recombinant equine interferon-beta 1 in whole blood and separated fractions of peripheral blood of horses.
Abstract: Interferon is known to induce antiviral mechanisms and to exert immunoregulatory capacities on various cell types. The antiviral capacity of recombinant equine interferon-beta 1 (rEqIFN-beta 1) is most sensitively monitored by indirect quantitation of multiplication of vesicular stomatitis virus (VSV) in blood cells of horses. As few as 0.5 pg rEqIFN-beta 1/ml can be assessed by means of 90% reduction of VSV-replication in whole blood (w.b.) as well as in isolated mononuclear blood cells (MNC) in spite of individual variations. The immunoregulatory influence of 20-50 pg rEqIFN-beta 1/ml is sufficient to cause at least a 50% reduction of mitogen-induced lymphocyte proliferation in MNC, while higher concentrations are needed in w.b. Of the mitogens tested the best stimulation of proliferation on the equine lymphoid cells was obtained with staphylococcal enterotoxin B (SEB). Release of reactive oxygen species (ROS) from phagocytic cells in w.b. or from isolated polymorphonuclear cells (PMN) as monitored by chemiluminescence (CL) does not seem suitable for evaluation of rEqIFN-beta 1-induced immunoregulation as only very high rEqIFN-beta 1-concentrations (10(3)-10(4) pg/ml) result in a minute increase (up to 20%) of CL. Comparative studies on w.b. and isolated leukocyte fractions from identical specimens of individual horses suggest that monitoring of antiviral and distinct immunoregulatory capacities of rEqIFN-beta 1 can be performed on w.b. without loss of information and sensitivity as compared to isolated MNC.
Publication Date: 1992-07-01 PubMed ID: 1325723DOI: 10.1111/j.1439-0450.1992.tb01177.xGoogle Scholar: Lookup
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- Journal Article
Summary
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This research investigates the antiviral and immunoregulatory effects of recombinant equine interferon-beta 1 (rEqIFN-beta 1) on the blood cells of horses. Evaluations conducted on whole blood versus isolated blood cells revealed no significant differences in understanding rEqIFN-beta 1’s impacts, suggesting whole blood testing is an effective monitoring approach.
Role of Interferon and the Monitoring Method
- Interferon is a substance with known antiviral mechanisms and immunoregulatory capacities, affecting multiple types of cells.
- The researchers used the multiplication of vesicular stomatitis virus (VSV) in horse blood cells to indirectly monitor the antiviral abilities of rEqIFN-beta 1.
Effects of rEqIFN-beta 1 and Testing Dosages
- Through testing, they found that as little as 0.5 pg rEqIFN-beta 1/ml resulted in significant VSV-reduction in both whole blood and isolated mononuclear blood cells (MNC). However, results varied between individual subjects.
- Regarding the immunoregulatory influence of rEqIFN-beta, between 20-50 pg rEqIFN-beta 1/ml was enough to cause at least a 50% reduction of mitogen-induced lymphocyte proliferation in MNC. Higher concentrations were required in whole blood testing.
- Of all the mitogens tested, staphylococcal enterotoxin B (SEB) most effectively stimulated proliferation of the equine lymphoid cells.
Reactive Oxygen Species Release and Monitoring Sensitivity
- The release of reactive oxygen species (ROS) from phagocytic cells in whole blood or from isolated polymorphonuclear cells (PMN) was monitored through chemiluminescence (CL). However, this method proved unsuitable for assessing rEqIFN-beta 1-mediated immunoregulation, as only very high rEqIFN-beta 1 concentrations led to minuscule upsurges in CL.
- Comparative investigations on whole blood and isolated leukocyte fractions from the same horse sample suggested that the monitoring of antiviral and some immunoregulatory capacities of rEqIFN-beta 1 could be accomplished using whole blood without significant loss of information or sensitivity compared to isolated MNC.
Cite This Article
APA
Marquardt J, Heymer J, Heinz H, Deegen E, Adolf GR, Leibold W.
(1992).
Monitoring of effects induced by recombinant equine interferon-beta 1 in whole blood and separated fractions of peripheral blood of horses.
Zentralbl Veterinarmed B, 39(5), 327-336.
https://doi.org/10.1111/j.1439-0450.1992.tb01177.x Publication
Researcher Affiliations
- Immunology Unit, Veterinary School, Hannover, Germany.
MeSH Terms
- Animals
- Female
- Horses / blood
- Interferon-beta / pharmacology
- Leukocyte Count / veterinary
- Leukocytes, Mononuclear / drug effects
- Lymphocyte Activation
- Male
- Recombinant Proteins / pharmacology
- Vesicular stomatitis Indiana virus / drug effects
Citations
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