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Monoclonal antibodies to the equine CD2 T lymphocyte marker, to a pan-granulocyte/monocyte marker and to a unique pan-B lymphocyte marker.
Abstract: Murine monoclonal antibodies, HB88A, B29A and DH59B separately identify the CD2 T lymphocyte molecule, a unique pan-B lymphocyte surface marker and a pan-granulocyte/monocyte surface molecule, respectively, in the horse. Specificity was shown by two-color immunofluorescent flow cytometry and immunofluorescent microscopy. MAb HB88A reacted with a 52 kDa pan-T lymphocyte molecule present on 75% +/- 7 of peripheral blood lymphocytes (PBL) (n = 15 horses). It also reacted with lymphocytes restricted to T lymphocyte dependent areas of lymph node and spleen. Specificity of mAb HB88A to CD2 was demonstrated by its reactivity to COS7 cells which expressed a transfected 1.5 kb equine lymphocyte c-DNA clone having 77.5% overall sequence homology with human CD2 c-DNA. MAb B29A reacted with a pan-B lymphocyte specific cell surface complex, 143, 72, 50, 40, 27 and 14.5 kDa, present on 19% +/- 7 of PBL (n = 15 horses). This complex has not been described in the horse or other species. MAb DH59B reacted with a 96 kDa pan-granulocyte/monocyte specific surface protein and identified macrophages and Kupffer cells in equine tissue sections. Together these mAbs can be used to identify and quantitate the major constituents of equine leukocytes.
Publication Date: 1994-12-01 PubMed ID: 7750989DOI: 10.1016/S0171-2985(11)80407-9Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research article discusses the identification of specific markers on T lymphocyte, pan-B lymphocyte and pan-granulocyte/monocyte blood cells in horses using Murine monoclonal antibodies HB88A, B29A and DH59B.
Understanding the research
The main components of this research paper are antibodies, cell markers and leukocytes:
- Antibodies: These are proteins that are produced by the immune system in response to foreign substances called antigens. Monoclonal antibodies (mAbs) like HB88A, B29A, and DH59B are antibodies made by identical immune cells, cloned from a single parent cell, and are directed against a specific cellular target.
- Cell Markers: Cellular markers or biological markers are substances that act as indicators of a biological state. It enables researchers to identify a particular category of cells or tissues.
- Leukocytes: Also known as white blood cells, are cells of the immune system involved in defending the body against both infectious disease and foreign invaders. Here, different types of leukocytes are being studied: T lymphocytes, B lymphocytes, and granulocytes/monocytes.
Detailed Explanation
The research involved the use of different mAbs to identify the presence and quantity of particular cell types within equine (horse) blood samples:
- HB88A and the CD2 T Lymphocyte Molecule: HB88A was found to react with a common T lymphocyte molecule present in approximately 75% of the tested peripheral blood lymphocytes (PBL) in 15 horses. Furthermore, the specificity of HB88A was confirmed through its reaction to COS7 cells which expressed a transfected equine lymphocyte c-DNA clone with demonstrated sequence homology to human CD2 c-DNA.
- B29A and Pan-B Lymphocyte Marker: B29A reacted with a specific type of cell surface complex present in about 19% of the PBL in the same set of horses. This complex had not been previously described in horses or any other species.
- DH59B and Granulocyte/ Monocyte Marker: DH59B reacted with a specific protein present on the surface of pan-granulocytes/ monocytes. Adding to this, it was able to identify macrophages and Kupffer cells within equine tissue sections.
The study concludes by showing that these mAbs can be used to not only identify but also quantify the primary constituents of equine leukocytes, providing valuable tools for further research into equine immunology.
Cite This Article
APA
Tumas DB, Brassfield AL, Travenor AS, Hines MT, Davis WC, McGuire TC.
(1994).
Monoclonal antibodies to the equine CD2 T lymphocyte marker, to a pan-granulocyte/monocyte marker and to a unique pan-B lymphocyte marker.
Immunobiology, 192(1-2), 48-64.
https://doi.org/10.1016/S0171-2985(11)80407-9 Publication
Researcher Affiliations
- Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, USA.
MeSH Terms
- Animals
- Antibodies, Monoclonal / immunology
- Antibody Specificity / immunology
- Antigens, Surface / immunology
- B-Lymphocytes / immunology
- CD2 Antigens / genetics
- CD2 Antigens / immunology
- Cell Line
- Fluorescent Antibody Technique
- Granulocytes / immunology
- Horses
- Leukocytes / immunology
- Lymphoid Tissue / immunology
- Mice
- Mice, Inbred BALB C
- Monocytes / immunology
- T-Lymphocytes / immunology
Grant Funding
- AI07025 / NIAID NIH HHS
- AI24291 / NIAID NIH HHS
Citations
This article has been cited 11 times.- Carossino M, Loynachan AT, Canisso IF, Cook RF, Campos JR, Nam B, Go YY, Squires EL, Troedsson MHT, Swerczek T, Del Piero F, Bailey E, Timoney PJ, Balasuriya UBR. Equine Arteritis Virus Has Specific Tropism for Stromal Cells and CD8(+) T and CD21(+) B Lymphocytes but Not for Glandular Epithelium at the Primary Site of Persistent Infection in the Stallion Reproductive Tract. J Virol 2017 Jul 1;91(13).
- Rees J, Haig D, Mack V, Davis WC. Characterisation of monoclonal antibodies specific for hamster leukocyte differentiation molecules. Vet Immunol Immunopathol 2017 Jan;183:40-44.
- Ramsay JD, Ueti MW, Johnson WC, Scoles GA, Knowles DP, Mealey RH. Lymphocytes and macrophages are infected by Theileria equi, but T cells and B cells are not required to establish infection in vivo. PLoS One 2013;8(10):e76996.
- Davis WC, Hamilton MJ. Use of flow cytometry to develop and characterize a set of monoclonal antibodies specific for rabbit leukocyte differentiation molecules. J Vet Sci 2008 Mar;9(1):51-66.
- Koo H, Ryu SH, Ahn HJ, Jung WK, Park YK, Kwon NH, Kim SH, Kim JM, Yoo BW, Choi SI, Davis WC, Park YH. Immunostimulatory effects of the anionic alkali mineral complex Barodon on equine lymphocytes. Clin Vaccine Immunol 2006 Nov;13(11):1255-66.
- Patton KM, McGuire TC, Fraser DG, Hines SA. Rhodococcus equi-infected macrophages are recognized and killed by CD8+ T lymphocytes in a major histocompatibility complex class I-unrestricted fashion. Infect Immun 2004 Dec;72(12):7073-83.
- Koo HC, Park YH, Hamilton MJ, Barrington GM, Davies CJ, Kim JB, Dahl JL, Waters WR, Davis WC. Analysis of the immune response to Mycobacterium avium subsp. paratuberculosis in experimentally infected calves. Infect Immun 2004 Dec;72(12):6870-83.
- Hines SA, Stone DM, Hines MT, Alperin DC, Knowles DP, Norton LK, Hamilton MJ, Davis WC, McGuire TC. Clearance of virulent but not avirulent Rhodococcus equi from the lungs of adult horses is associated with intracytoplasmic gamma interferon production by CD4+ and CD8+ T lymphocytes. Clin Diagn Lab Immunol 2003 Mar;10(2):208-15.
- Mealey RH, Fraser DG, Oaks JL, Cantor GH, McGuire TC. Immune reconstitution prevents continuous equine infectious anemia virus replication in an Arabian foal with severe combined immunodeficiency: lessons for control of lentiviruses. Clin Immunol 2001 Nov;101(2):237-47.
- Lonning SM, Zhang W, McGuire TC. Gag protein epitopes recognized by CD4(+) T-helper lymphocytes from equine infectious anemia virus-infected carrier horses. J Virol 1999 May;73(5):4257-65.
- Wong DM, Alcott CJ, Clark SK, Jones DE, Fisher PG, Sponseller BA. Alloimmune neonatal neutropenia and neonatal isoerythrolysis in a Thoroughbred colt. J Vet Diagn Invest 2012 Jan;24(1):219-26.
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