Morphogenesis of Berne virus (proposed family Toroviridae).
Abstract: In equine dermis cells infected with Berne virus particles were first detected 10 h after infection. Virions were encountered in all parts of the Golgi system and, infrequently, in the rough endoplasmic reticulum. A unique form of budding of preassembled rigid tubular nucleocapsids was demonstrated. Masses of tubular nucleocapsids of a lesser diameter and electron density were prominent in the cytoplasm and the nucleus of infected cells. Within the Golgi system and cytoplasmic cisternae virions appeared as straight or slightly curved rods. Extremely long, aberrant virions (250 nm) were occasionally seen. The proper torovirion morphology was observed in extracellular particles and in vacuoles near the cell surface.
Publication Date: 1986-07-01 PubMed ID: 3723110DOI: 10.1099/0022-1317-67-7-1305Google Scholar: Lookup
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- Journal Article
Summary
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The research article discusses the morphogenesis or the development of form and structure of Berne virus in equine dermis cells, witnessing the first indication of virus particles 10 hours post infection and identifying the role of different cellular components in this process.
Morphogenesis of Berne virus
The research essentially explores the morphogenesis of the Berne virus, which is proposed to be part of the Toroviridae family. It takes place in equine dermis cells, specifically examining how the virus forms and develops.
- The study observed the development of Berne virus particles about 10 hours post infection.
- The researchers primarily found the virions, or virus particles, in all sections of the Golgi system – an organelle that processes and packages proteins for secretion out of the cell.
- In fewer instances, virions were also detected in the rough endoplasmic reticulum – the part of the cell responsible for protein synthesis and processing.
The unique budding process
The study also unearthed a unique form of virus replication method or “budding” process involving pre-assembled rigid tubular nucleocapsids.
- These nucleocapsids are protein shells that contain the viral genome.
- Masses of these nucleocapsids, which were observed to have a smaller diameter and electron density, were prominent within the cell’s nucleus and cytoplasm.
Virus particle formation
In terms of the formation of virus particles, diverse observations were made within different parts of the cell.
- Inside the Golgi system and cytoplasmic cisternae (a network of membrane vesicles), virus particles appeared as straight or somewhat curved rods.
- The researchers occasionally observed extremely long, abnormal virus particles, up to 250 nm in length.
- Interestingly, the correct or ideal morphology of the virus, referred to as the “torovirion” form, was noticed in virus particles outside the cell, as well as inside vacuoles near the cell’s outer surface.
Cite This Article
APA
Weiss M, Horzinek MC.
(1986).
Morphogenesis of Berne virus (proposed family Toroviridae).
J Gen Virol, 67 ( Pt 7), 1305-1314.
https://doi.org/10.1099/0022-1317-67-7-1305 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Capsid / ultrastructure
- Cells, Cultured
- Horses / microbiology
- Microscopy, Electron
- Morphogenesis
- RNA Viruses / isolation & purification
- RNA Viruses / physiology
- RNA Viruses / ultrastructure
- Skin / microbiology
- Virion / ultrastructure
- Virus Replication
Citations
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