FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Reprod Domest Anim / Motility and plasma membrane integrity of spermatozoa in fra...
Reproduction in domestic animals = Zuchthygiene2006; 41(1); 33-38; doi: 10.1111/j.1439-0531.2006.00647.x

Motility and plasma membrane integrity of spermatozoa in fractionated stallion ejaculates after storage.

Abstract: With the aim of investigating properties of stallion seminal plasma to eventually improve semen-handling techniques, sperm motility and plasma membrane integrity were analysed in different fractions of the ejaculates after storage. Semen was collected using a computer-controlled automated phantom that separates the ejaculates into five successive cups. Samples containing seminal plasma and skim milk extender were compared with samples stored in skim milk extender after the removal of seminal plasma by centrifugation. Fractionated ejaculates were stored cooled for 24 h after dilution with extender (Expt 1) or frozen in liquid nitrogen (Expt 2). In Expt 1, cup 1 was pre-sperm fluid, cups 2 and 3 sperm-rich fractions, and cup 4 sperm-poor fractions. In Expt 2, cups 1 and 2 were sperm-rich fractions, and cups 3 and 4 sperm-poor fractions. One sample (WE) represented the whole ejaculate in both experiments. Motility parameters were determined with a Hamilton-Thorn Motility Analyzer, and plasma membrane integrity was assessed using carboxyfluorescein diacetate and propidium iodide staining and fluorescence microscopy. The removal of seminal plasma lowered motility values, but not plasma membrane integrity, in both experiments. No significant differences between cups were observed after cooled storage. The cups differed significantly in most post-thaw motility parameters, and the sperm-rich fraction showed higher post-thaw motility than the whole ejaculate.
Get Full Text
Publication Date: 2006-01-20 PubMed ID: 16420325DOI: 10.1111/j.1439-0531.2006.00647.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study analyzes the motion and membrane integrity of sperm cells in different parts of horse ejaculates following periods of storage. The study aimed to understand features of horse seminal plasma to potentially enhance semen-handling techniques.

Methodology Used in the Study

  • The researchers used a computer-controlled automated phantom to collect and separate horse semen into five consecutive cups.
  • The fractionated ejaculates were stored under cool temperatures for 24 hours after being diluted with an extender in the first experiment, whereas they were frozen in liquid nitrogen in the second experiment.
  • The first two categories represented pre-sperm fluid and the sperm-rich fractions, respectively. The fourth group was indicative of sperm-poor fractions.
  • The researchers used a Hamilton-Thorn Motility Analyzer to determine the motility parameters of the sperm.
  • Plasma membrane integrity was investigated using carboxyfluorescein diacetate and propidium iodide staining and fluorescence microscopy.

Significant Findings from the Experiments

  • Removing the seminal plasma led to lower motility values in both experiments, although it did not affect the plasma membrane integrity.
  • No considerable differences between the cups were noted after they were stored in a cool environment.
  • After thawing, the cups fared differently in terms of most post-thaw motility parameters.
  • The sperm-rich fraction demonstrated greater post-thaw motility compared to the whole ejaculate.

Implications of the Study

  • The results suggest that the sperm-rich fraction of an ejaculate could be preferable for storage and potential future use due to its higher post-thaw motility.
  • The study shows that removing seminal plasma can impact sperm motility, indicating that techniques that preserve seminal plasma could be beneficial.
  • The findings could potentially help in improving semen-handling and storage techniques in veterinary assisted reproduction.

Cite This Article

APA
Kareskoski AM, Reilas T, Andersson M, Katila T. (2006). Motility and plasma membrane integrity of spermatozoa in fractionated stallion ejaculates after storage. Reprod Domest Anim, 41(1), 33-38. https://doi.org/10.1111/j.1439-0531.2006.00647.x

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 0936-6768
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 41
Issue: 1
Pages: 33-38

Researcher Affiliations

Kareskoski, A M
  • Saari Unit, Faculty of Veterinary Medicine, University of Helsinki, Saarentaus, Finland. maria.kareskosi@helsinki.fi
Reilas, T
    Andersson, M
      Katila, T

        MeSH Terms

        • Animals
        • Cell Membrane / physiology
        • Cell Membrane / ultrastructure
        • Cryopreservation / methods
        • Cryopreservation / veterinary
        • Horses / physiology
        • Male
        • Semen Preservation / methods
        • Semen Preservation / veterinary
        • Sperm Count / veterinary
        • Sperm Motility
        • Spermatozoa / physiology
        • Spermatozoa / ultrastructure
        • Time Factors
        • Tissue and Organ Harvesting / methods
        • Tissue and Organ Harvesting / veterinary

        Citations

        This article has been cited 6 times.
        1. Marzano G, Moscatelli N, Di Giacomo M, Martino NA, Lacalandra GM, Dell'Aquila ME, Maruccio G, Primiceri E, Chiriacò MS, Zara V, Ferramosca A. Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm. Biology (Basel) 2020 Jan 27;9(2).
          doi: 10.3390/biology9020022pubmed: 32012799google scholar: lookup
        2. Papas M, Catalán J, Fernandez-Fuertes B, Arroyo L, Bassols A, Miró J, Yeste M. Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance. Antioxidants (Basel) 2019 Nov 9;8(11).
          doi: 10.3390/antiox8110539pubmed: 31717586google scholar: lookup
        3. Morrell JM, Johannisson A. Comparison of the Effect of Heterologous and Homologous Seminal Plasma on Motility and Chromatin Integrity of Stallion Spermatozoa Selected by Single Layer Centrifugation. J Vet Med 2014;2014:325451.
          doi: 10.1155/2014/325451pubmed: 26464926google scholar: lookup
        4. Khalil WA, Mostafa HE, Derbala MK, Alfattah MA, Alhujaili W, Hassan MAE, El-Harairy MA, Abdelnour SA. Efficacy of butylated hydroxytoluene nanoparticles in enhancing the quality and preservation of stallion chilled semen. Vet Res Commun 2025 Dec 3;50(1):62.
          doi: 10.1007/s11259-025-10977-5pubmed: 41335269google scholar: lookup
        5. Mappanganro R, Sonjaya H, Baco S, Hasbi H, Gustina S. Seminal plasma protein profiles based on molecular weight as biomarkers of sperm fertility in horned and polled Bali bulls. Vet World 2025 Jan;18(1):122-132.
          doi: 10.14202/vetworld.2025.122-132pubmed: 40041500google scholar: lookup
        6. Seyedasgari F, Asadi B, Kim E. Seminal plasma modulates post-thaw longevity and motility of frozen sperm in dromedary camel. Anim Biosci 2023 Dec;36(12):1821-1830.
          doi: 10.5713/ab.23.0136pubmed: 37641835google scholar: lookup
        Subscribe to our newsletter
        Join 99,357 horse owners like you who receive our email updates on horse health and nutrition.