Multipotency of equine mesenchymal stem cells derived from synovial fluid.
Abstract: Cartilage regeneration with cell therapy following arthroscopic surgery could be used in racehorses with intra-articular fractures (IAF) and osteochondritis dissecans (OCD). The aims of this study were to investigate the origin and multipotency of stromal cells in the synovial fluid (SF) of horses with intra-articular injury and synovitis, and to provide a new strategy for regeneration of lost articular cartilage. Mesenchymal stromal cells were isolated from SF of horses with IAF and OCD. Multipotency was analysed by RT-PCR for specific mRNAs and staining for production of specific extracellular matrices after induction of differentiation. The total number of SF-derived mesenchymal stromal cells reached >1 × 10(7) by the fourth passage. SF-derived cells were strongly positive (>90% cells positive) for CD44, CD90 and major histocompatibility complex (MHC) class I, and moderately positive (60-80% cells positive) for CD11a/CD18, CD105 and MHC class II by flow cytometry. SF-derived cells were negative for CD34 and CD45. Under specific nutrient conditions, SF-derived cells differentiated into osteogenic, chondrogenic, adipogenic and tenogenic lineages, as indicated by the expression of specific marker genes and by the production of specific extracellular matrices. Chondrogenic induction in culture resulted in a change in cell shape to a 'stone-wall' appearance and formation of a gelatinous sheet that was intensely stained with Alcian blue. SF may be a novel source of multipotent mesenchymal stem cells with the ability to regenerate chondrocytes.
Copyright © 2014 Elsevier Ltd. All rights reserved.
Publication Date: 2014-08-04 PubMed ID: 25151209DOI: 10.1016/j.tvjl.2014.07.029Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Arthroscopy
- Articular Cartilage
- Cartilage
- Cell Culture
- Cell Proliferation
- Cells
- Equine Diseases
- Equine Health
- Extracellular matrix
- Flow Cytometry
- Horses
- In Vitro Research
- Major Histocompatibility Complex (MHC)
- Mesenchymal Cells
- Osteoarthritis
- Regenerative Medicine
- Stem Cells
- Synovial Fluid
- Veterinary Medicine
- Veterinary Research
Summary
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This research article reveals that synovial fluid, found in the joints of horses, could provide a new source of mesenchymal stem cells. These cells have demonstrated the potential for multipotency and could therefore provide new avenues in regenerating damaged articular cartilage, particularly in racehorses with joint injuries.
Objective of the Research
- The main goal of this study was to explore the origin and multipotency of stromal cells present in the synovial fluid of horses suffering from intra-articular injuries and inflammation. It also aimed to propose a new approach to facilitate the regeneration of deteriorated articular cartilage.
Methodology
- For the study, Mesenchymal stromal cells were isolated from the synovial fluid of horses that were diagnosed with intra-articular fractures (IAF) and osteochondritis dissecans (OCD).
- For analyzing the multipotency, the researchers employed RT-PCR (reverse transcription-polymerase chain reaction) to detect the presence of specific mRNA’s. They also utilized staining methods to identify the production of specific extracellular matrixes following the induction of differentiation.
Findings
- The study revealed that the number of synovial fluid-derived mesenchymal stromal cells were more than 10 million by the fourth passage.
- Flow cytometry showed a strong positivity in synovial fluid-derived cells for CD44, CD90, and major histocompatibility complex class I, while a moderate positivity for CD11a/CD18, CD105, and major histocompatibility complex class II. However, the cells were negative for CD34 and CD45.
- Under nutrient-specific conditions, the synovial fluid-derived cells differentiated into osteogenic, chondrogenic, adipogenic, and tenogenic lineages, exemplified by the expression of specific marker genes and by the creation of specific extracellular matrices.
- After inducing chondrogenic differentiation in a culture environment, the cells changed their shape into a ‘stone-wall’ appearance and formed a gelatinous sheet that stained intensely with Alcian blue, a dye specifically used for staining acidic polysaccharides such as glycosaminoglycans in cartilages and other body tissues.
Conclusion and Implications
- The research concludes with the significant finding that synovial fluid can be a new source of multipotent mesenchymal stem cells that have the ability to regenerate damaged chondrocytes – a type of cell found in healthy cartilage.
- This discovery has vast implications for cell therapy and could be particularly beneficial in treating racehorses with osteochondritis dissecans or intra-articular fractures, conditions that often lead to joint injuries in these animals.
- These findings could help develop effective and novel cell-based therapies for cartilage repair and regeneration in the field of veterinary medicine and potentially in human orthopedic treatments as well.
Cite This Article
APA
Murata D, Miyakoshi D, Hatazoe T, Miura N, Tokunaga S, Fujiki M, Nakayama K, Misumi K.
(2014).
Multipotency of equine mesenchymal stem cells derived from synovial fluid.
Vet J, 202(1), 53-61.
https://doi.org/10.1016/j.tvjl.2014.07.029 Publication
Researcher Affiliations
- Faculty of Veterinary Medicine, Kagoshima University, 21-24 Korimoto 1-chome, Kagoshima 890-0065, Japan.
- Hidaka Horse Breeders Association, 175-2 Shizunai-Shinmori, Shinhidaka-cyo, Hidaka-gun, Hokkaido 056-0002, Japan.
- Kyushu Stallion Station, The Japan Bloodhorse Bleeders' Association, 3995 Nagata, Osaki-cho, Soo-gun, Kagoshima 899-8313, Japan.
- Faculty of Veterinary Medicine, Kagoshima University, 21-24 Korimoto 1-chome, Kagoshima 890-0065, Japan.
- Faculty of Veterinary Medicine, Kagoshima University, 21-24 Korimoto 1-chome, Kagoshima 890-0065, Japan.
- Faculty of Veterinary Medicine, Kagoshima University, 21-24 Korimoto 1-chome, Kagoshima 890-0065, Japan.
- Graduate School of Science and Engineering, Saga University, 1 Honjyo-cho, Saga 840-8502, Japan.
- Faculty of Veterinary Medicine, Kagoshima University, 21-24 Korimoto 1-chome, Kagoshima 890-0065, Japan. Electronic address: kaz_msm@vet.kagoshima-u.ac.jp.
MeSH Terms
- Adipose Tissue / cytology
- Animals
- Biomarkers
- Bone Marrow Cells / cytology
- Bone Marrow Cells / physiology
- Cell Culture Techniques
- Female
- Horses
- Male
- Mesenchymal Stem Cells / physiology
- Synovial Fluid / cytology
Citations
This article has been cited 19 times.- Soukup R, Gerner I, Gültekin S, Baik H, Oesterreicher J, Grillari J, Jenner F. Characterisation of Extracellular Vesicles from Equine Mesenchymal Stem Cells.. Int J Mol Sci 2022 May 23;23(10).
- Hart DA. One of the Primary Functions of Tissue-Resident Pluripotent Pericytes Cells May Be to Regulate Normal Organ Growth and Maturation: Implications for Attempts to Repair Tissues Later in Life.. Int J Mol Sci 2022 May 14;23(10).
- Murata D, Ishikawa S, Sunaga T, Saito Y, Sogawa T, Nakayama K, Hobo S, Hatazoe T. Osteochondral regeneration of the femoral medial condyle by using a scaffold-free 3D construct of synovial membrane-derived mesenchymal stem cells in horses.. BMC Vet Res 2022 Jan 22;18(1):53.
- Zayed M, Adair S, Dhar M. Effects of Normal Synovial Fluid and Interferon Gamma on Chondrogenic Capability and Immunomodulatory Potential Respectively on Equine Mesenchymal Stem Cells.. Int J Mol Sci 2021 Jun 15;22(12).
- Mazzotti E, Teti G, Falconi M, Chiarini F, Barboni B, Mazzotti A, Muttini A. Age-Related Alterations Affecting the Chondrogenic Differentiation of Synovial Fluid Mesenchymal Stromal Cells in an Equine Model.. Cells 2019 Sep 20;8(10).
- Yamasaki A, Omura T, Murata D, Kobayashi M, Sunaga T, Kusano K, Ueno Y, Kuramoto T, Hobo S, Misumi K. A pilot study of regenerative therapy by implanting synovium-derived mesenchymal stromal cells in equine osteochondral defect models.. J Equine Sci 2018 Dec;29(4):117-122.
- Neybecker P, Henrionnet C, Pape E, Mainard D, Galois L, Loeuille D, Gillet P, Pinzano A. In vitro and in vivo potentialities for cartilage repair from human advanced knee osteoarthritis synovial fluid-derived mesenchymal stem cells.. Stem Cell Res Ther 2018 Nov 28;9(1):329.
- Boere J, Malda J, van de Lest CHA, van Weeren PR, Wauben MHM. Extracellular Vesicles in Joint Disease and Therapy.. Front Immunol 2018;9:2575.
- Jiao Y, Li XY, Liu J. A New Approach to Cerebral Palsy Treatment: Discussion of the Effective Components of Umbilical Cord Blood and its Mechanisms of Action.. Cell Transplant 2019 May;28(5):497-509.
- Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance.. Cell Transplant 2018 Jan;27(1):93-116.
- Desancé M, Contentin R, Bertoni L, Gomez-Leduc T, Branly T, Jacquet S, Betsch JM, Batho A, Legendre F, Audigié F, Galéra P, Demoor M. Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy.. Int J Mol Sci 2018 Feb 10;19(2).
- Ishikawa S, Horinouchi C, Mizoguchi R, Senokuchi A, Kamikakimoto R, Murata D, Hatazoe T, Tozaki T, Misumi K, Hobo S. Isolation of equine peripheral blood stem cells from a Japanese native horse.. J Equine Sci 2017;28(4):153-158.
- Zayed M, Caniglia C, Misk N, Dhar MS. Donor-Matched Comparison of Chondrogenic Potential of Equine Bone Marrow- and Synovial Fluid-Derived Mesenchymal Stem Cells: Implications for Cartilage Tissue Regeneration.. Front Vet Sci 2016;3:121.
- Kim R, Park SI, Lee CY, Lee J, Kim P, Oh S, Lee H, Lee MY, Kim J, Chung YA, Hwang KC, Maeng LS, Chang W. Alternative new mesenchymal stem cell source exerts tumor tropism through ALCAM and N-cadherin via regulation of microRNA-192 and -218.. Mol Cell Biochem 2017 Mar;427(1-2):177-185.
- Ishikawa S, Horinouchi C, Murata D, Matsuzaki S, Misumi K, Iwamoto Y, Korosue K, Hobo S. Isolation and characterization of equine dental pulp stem cells derived from Thoroughbred wolf teeth.. J Vet Med Sci 2017 Jan 20;79(1):47-51.
- Liu S, Zhou J, Zhang X, Liu Y, Chen J, Hu B, Song J, Zhang Y. Strategies to Optimize Adult Stem Cell Therapy for Tissue Regeneration.. Int J Mol Sci 2016 Jun 21;17(6).
- Murata D, Yamasaki A, Matsuzaki S, Sunaga T, Fujiki M, Tokunaga S, Misumi K. Characteristics and multipotency of equine dedifferentiated fat cells.. J Equine Sci 2016;27(2):57-65.
- Fülber J, Maria DA, da Silva LC, Massoco CO, Agreste F, Baccarin RY. Comparative study of equine mesenchymal stem cells from healthy and injured synovial tissues: an in vitro assessment.. Stem Cell Res Ther 2016 Mar 5;7:35.
- Prado AA, Favaron PO, da Silva LC, Baccarin RY, Miglino MA, Maria DA. Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane.. BMC Vet Res 2015 Nov 10;11:281.
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