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Equine veterinary journal. Supplement2011; (38); 303-310; doi: 10.1111/j.2042-3306.2010.00267.x

Muscular microRNA expressions in healthy and myopathic horses suffering from polysaccharide storage myopathy or recurrent exertional rhabdomyolysis.

Abstract: MicroRNAs (miRNA) are small endogenous noncoding interfering RNA molecules (18-25 nucleotides) regarded as major regulators in eukaryotic gene expression. They play a role in developmental timing, cellular differentiation, signalling and apoptosis pathways. Because of the central function of miRNAs in the proliferation and differentiation of the myoblasts demonstrated in mouse and man, it is assumed that they could be present in equine muscles and their expression profile may be related to the muscle status. Objective: To identify miRNA candidates in the muscles of control and affected horses suffering from polysaccharide storage myopathy (PSSM) and recurrent exertional rhabdomyolysis (RER). Methods: Muscle biopsies were collected in the gluteus medius of horses allocated into 4 groups: French Trotters (3 control-TF vs. 3 RER-TF) and Norman Cob (5 control-Cob vs. 9 PSSM-Cob). Blood samples were collected for miRNA analysis. Total RNA were extracted and real time quantitative RT-QPCR analysis were conducted using 10 miRNA assays (mir-1-23-30-133-181-188-195-206-339-375). Results: All the miRNA candidates were significantly detected in the muscles and some in blood samples. Variance analysis revealed highly significant (P < 0.0001) effects of the miRNA type, breed and pathology on the miRNA expression. A specific miRNA profile was related to each myopathy: a higher expression of mir-1, 133, 23a, 30b, 195 and 339 in RER-TF vs. control-TF (P < 0.05); a higher expression of mir-195 in PSSM-Cob vs. control-Cob (P < 0.05). The miRNA profile was different between breeds for mir-181, 188 and 206 (P < 0.05). The mir-1, 133, 181, 195 and 206 were detected in blood of control-Cob and PSSM-Cob horses. Conclusions: This first study about muscular miRNA profile in equine myopathies indicated that it is possible to discriminate pathological from control horses according to their miRNA profile. The RER miRNA profile was more specific and contrasted than the PSSM profile.
© 2010 EVJ Ltd.
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Publication Date: 2011-05-27 PubMed ID: 21059022DOI: 10.1111/j.2042-3306.2010.00267.xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explores the role of microRNAs (miRNA) in the muscles of healthy and myopathic horses. It investigates the relationship between miRNA expressions and two specific types of horsey muscle diseases, polysaccharide storage myopathy (PSSM) and recurrent exertional rhabdomyolysis (RER). The study found that different muscle diseases exhibit different miRNA expressions and these varied expressions are possibly significant enough to distinguish between healthy and diseased horses.

Objective and Methodology

The study aimed to identify potential miRNAs in the muscles of both healthy horses and those affected with PSSM or RER. To accomplish this objective, muscle samples were gathered from the gluteus medius of horses sorted into four groups, comprising of French Trotters and Norman Cob, both healthy and affected by either of the two diseases. Five types of miRNA assays were used to conduct a quantitative real-time PCR analysis on the extracted total RNA from these samples.

Key Findings

  • All the miRNA candidates were detected significantly within the muscle samples, and occasionally in blood samples.
  • It was found that the type of miRNA, the breed of the horse, and the pathology significantly influenced the miRNA expression. In other words, specific miRNA expressions were associated with specific forms of myopathy.
  • For example, higher levels of mir-1, 133, 23a, 30b, 195, and 339 were detected in RER-affected French Trotters compared to control groups. Similarly, higher levels of mir-195 were found in PSSM-affected Norman Cob horses compared to the control group.
  • Moreover, miRNA expressions also varied depending on the breed of the horse, which was evident from the different expressions of mir-181, 188, and 206.

Conclusions and Implications

This study is among the first to explore the muscular miRNA profile in equine myopathies. The results suggest a possible correlation between muscular miRNAs and particular muscle pathologies. These findings propose that these miRNAs could potentially serve as markers to differentiate between healthy and disease-affected horses, thus offering valuable diagnostic insights. The more unique and contrasting miRNA profile of RER compared to PSSM further strengthens this observation. However, further exploration and validation of these findings would be necessary for diagnostic application.

Cite This Article

APA
Barrey E, Bonnamy B, Barrey EJ, Mata X, Chaffaux S, Guerin G. (2011). Muscular microRNA expressions in healthy and myopathic horses suffering from polysaccharide storage myopathy or recurrent exertional rhabdomyolysis. Equine Vet J Suppl(38), 303-310. https://doi.org/10.1111/j.2042-3306.2010.00267.x

Publication

Equine veterinary journal. Supplement
NlmUniqueID: 9614088
Country: United States
Language: English
Issue: 38
Pages: 303-310

Researcher Affiliations

Barrey, E
  • Unité de Biologie Intégrative des Adaptations à l'Exercice, INSERM 902, Genopole Evry, France. eric.barrey@inserm.fr
Bonnamy, B
    Barrey, E J
      Mata, X
        Chaffaux, S
          Guerin, G

            MeSH Terms

            • Animals
            • Gene Expression Regulation
            • Genotype
            • Horse Diseases / metabolism
            • Horses
            • Male
            • MicroRNAs / genetics
            • MicroRNAs / metabolism
            • Muscle, Skeletal / metabolism
            • Muscle, Skeletal / pathology
            • Muscular Diseases / genetics
            • Muscular Diseases / metabolism
            • Polymerase Chain Reaction
            • Rhabdomyolysis / metabolism
            • Rhabdomyolysis / veterinary

            Citations

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